FastQCFastQC Report
Thu 26 May 2016
SRR1512272_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512272_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1905164
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT73330.38490124734668507No Hit
CTGTAGGACGTGGAATATGGCAAGA54970.2885315909811439No Hit
GTCCTACAGTGGACATTTCTAAATT53910.282967765504702No Hit
GTCCTAAAGTGTGTATTTCTCATTT51010.26774597882387025No Hit
GGTATCAACGCAGAGTACTTTTTTT47580.24974227940481766No Hit
TATCAACGCAGAGTACTTTTTTTTT45220.237354894381796No Hit
CTTTAGGACGTGAAATATGGCGAGG44450.23331324757343724No Hit
GTCCTACAGTGTGCATTTCTCATTT29500.1548423127877705No Hit
CTGAAGGACCTGGAATATGGCGAGA24990.1311698100530978No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22710.11920233638678873No Hit
CTGTAGGACCTGGAATATGGCGAGA22370.1174177131207602No Hit
GTCCTTCAGTGTGCATTTCTCATTT19950.10471539458020412No Hit
ATTTAGAAATGTCCACTGTAGGACG19180.10067374777184537No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG350.002167417216.2891145
GGTATCA13400.014.6190471
TAGGACC9550.013.630764
GCCGGTT905.4089105E-712.66631711
GGCGAGG11000.011.99885719
TGTACCG752.0702655E-411.402385
CCAACGA1001.9411764E-611.39459819
TCCAACG1604.5474735E-1111.27501618
AGGACCT18400.011.2061435
TACGAGT600.00586850611.0853564
CAAGACG600.00586850611.0853564
TAGGACT3100.011.0342714
TATGACT1900.011.00200754
CGGTTTC951.3621351E-510.99882913
AATCCCG2600.010.95634419
ACCTTTT7600.010.870131515
GAGAGCG700.001486791610.8614067
TGTAGGA31600.010.8545442
TAGAAAT6500.010.8173584
ATTACAC1951.8189894E-1210.7196243
Produced by FastQC (version 0.11.5)