FastQCFastQC Report
Thu 26 May 2016
SRR1512271_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512271_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1633723
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60480.3701973957641534No Hit
GTCCTACAGTGGACATTTCTAAATT48220.2951540744667242No Hit
GTCCTAAAGTGTGTATTTCTCATTT45390.2778316764837124No Hit
CTGTAGGACGTGGAATATGGCAAGA43560.26663026718727717No Hit
CTTTAGGACGTGAAATATGGCGAGG38360.23480112601707878No Hit
GGTATCAACGCAGAGTACTTTTTTT37440.2291698164254283No Hit
TATCAACGCAGAGTACTTTTTTTTT35860.21949865430063725No Hit
GTCCTACAGTGTGCATTTCTCATTT22510.1377834553348395No Hit
CTGAAGGACCTGGAATATGGCGAGA21280.13025463925035027No Hit
GTCCTTCAGTGTGCATTTCTCATTT18690.11440127855211685No Hit
ATTTAGAAATGTCCACTGTAGGACG17560.10748456133628527No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16680.10209809129209788No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16680.10209809129209788No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTCG400.005304587514.23971611
GGTATCA10350.013.8622611
AAGGCGT853.9361366E-612.2962086
TAGGACC6500.012.2793894
CGTGCGC802.8941742E-511.86387610
TATCGCG1104.9552364E-711.2295287
GCGTGCG951.3866167E-510.980929
CGCGCCT1158.883835E-710.73269812
GCATAAG1252.234392E-710.6418091
TCGCGCG1252.2938366E-710.6215449
GTCCTAC23300.010.5211031
GTATCAA23650.010.4859271
GGCGAGG10800.010.38427219
TAGACTG1659.258656E-1010.3657185
CTACACT2950.010.3071554
ATCGCGC1201.5626156E-610.2712918
TGGTATC3000.010.1356812
GTCCTAT2350.010.1081021
TCTACAC2450.010.0839683
GTCCTAA14250.010.0683781
Produced by FastQC (version 0.11.5)