##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512271_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1633723 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.214144013397622 31.0 30.0 34.0 16.0 34.0 2 30.082119184219113 31.0 30.0 34.0 25.0 34.0 3 30.7386294983911 33.0 31.0 34.0 27.0 34.0 4 34.405248013280094 37.0 35.0 37.0 32.0 37.0 5 34.574946303626746 37.0 35.0 37.0 32.0 37.0 6 34.72491236274448 37.0 35.0 37.0 32.0 37.0 7 34.661235717438025 37.0 35.0 37.0 32.0 37.0 8 34.664452909091686 37.0 35.0 37.0 32.0 37.0 9 36.263953558834636 39.0 37.0 39.0 32.0 39.0 10 36.20144908286166 39.0 37.0 39.0 32.0 39.0 11 36.22611850356517 39.0 37.0 39.0 32.0 39.0 12 36.17252985971306 39.0 37.0 39.0 32.0 39.0 13 36.1489199821512 39.0 37.0 39.0 32.0 39.0 14 37.40043140728263 40.0 38.0 41.0 32.0 41.0 15 37.330309972988076 40.0 38.0 41.0 32.0 41.0 16 37.26312844955969 40.0 38.0 41.0 32.0 41.0 17 37.22641047472552 40.0 38.0 41.0 32.0 41.0 18 37.0075110652173 40.0 38.0 41.0 31.0 41.0 19 37.121298408604154 40.0 38.0 41.0 31.0 41.0 20 37.058236310561824 40.0 38.0 41.0 31.0 41.0 21 36.99931628556371 40.0 38.0 41.0 31.0 41.0 22 36.93512180461437 40.0 38.0 41.0 31.0 41.0 23 36.814426925494715 40.0 38.0 41.0 31.0 41.0 24 36.69326685123488 40.0 38.0 41.0 30.0 41.0 25 36.57846770841813 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9210671355383369 1101 2 0.2477329265091015 1101 3 -0.2766997096252446 1101 4 -0.24145403898688755 1101 5 -0.5581434979578219 1101 6 -0.5886204917888094 1101 7 -0.4597107470792423 1101 8 -0.6287540258711246 1101 9 -0.5610818548441969 1101 10 -0.7592504952365573 1101 11 -0.7205265917732504 1101 12 -0.862281186662976 1101 13 -0.9240246671132653 1101 14 -1.209151737923932 1101 15 -1.1537960258840343 1101 16 -1.2525948239543894 1101 17 -1.2435453313530616 1101 18 -1.1500031776334154 1101 19 -1.3819378257368697 1101 20 -1.3170844061352227 1101 21 -1.5500942763289416 1101 22 -1.3854342068158445 1101 23 -1.5609267243763938 1101 24 -1.7300324271953897 1101 25 -1.7559193039972456 1102 1 0.9645916896613933 1102 2 0.09355915886900235 1102 3 -0.28992597472183235 1102 4 -0.36422946849548765 1102 5 -0.5836147297881595 1102 6 -0.5618550373425535 1102 7 -0.5602629380304336 1102 8 -0.7920659840708666 1102 9 -0.9201399688898491 1102 10 -0.9155706023047898 1102 11 -0.9448170560886524 1102 12 -1.2260965517252203 1102 13 -0.9346977912527237 1102 14 -1.434953867092645 1102 15 -1.3676925907781055 1102 16 -1.3690868949352435 1102 17 -1.466125945454877 1102 18 -1.364084287599816 1102 19 -1.3974949753339203 1102 20 -1.5593118491107916 1102 21 -1.5759514480714003 1102 22 -1.4426359579078678 1102 23 -1.8072157636828905 1102 24 -1.6920145375565525 1102 25 -1.6754183363391704 1103 1 1.0681343398011123 1103 2 0.39069454917552093 1103 3 -0.20724675640845192 1103 4 -0.20520666879117755 1103 5 -0.3400822632914213 1103 6 -0.41699578326372944 1103 7 -0.5012675912191114 1103 8 -0.47043387124100633 1103 9 -0.6388789455564208 1103 10 -0.6630032200224889 1103 11 -0.6128126574910482 1103 12 -0.6898372614515225 1103 13 -0.7009080344025378 1103 14 -1.0227241400572211 1103 15 -0.9402226739343007 1103 16 -1.005896623381652 1103 17 -1.1053090455153054 1103 18 -1.0771593796973704 1103 19 -1.2533113078114582 1103 20 -1.202500924393071 1103 21 -1.1589527394309442 1103 22 -1.355669333877536 1103 23 -1.1848065346030694 1103 24 -1.3131983553797681 1103 25 -1.3259943869874604 1104 1 1.1326317724179127 1104 2 0.4477052872493097 1104 3 0.0010293005572776792 1104 4 -0.08157752008217756 1104 5 -0.29493667986198346 1104 6 -0.22150240961373413 1104 7 -0.2355270123976041 1104 8 -0.39987123100152644 1104 9 -0.45084791707576954 1104 10 -0.46976437701618323 1104 11 -0.5005277344018637 1104 12 -0.3418644205953427 1104 13 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-0.021144396370964103 1107 11 0.016865411441536082 1107 12 0.0893911004523531 1107 13 -0.14374619696137358 1107 14 -0.12399515276734974 1107 15 -0.002011329795543304 1107 16 -0.05281095328444252 1107 17 -0.01974015185168554 1107 18 0.20584724783726216 1107 19 -0.04551188804510531 1107 20 -0.021777345005375537 1107 21 0.04144844179605656 1107 22 -0.20814570674252764 1107 23 -0.20418571573664934 1107 24 -0.043498696357865185 1107 25 -0.17477034310145 1108 1 -0.14981927393074912 1108 2 -0.26428077448097653 1108 3 -0.6883323771634231 1108 4 -0.43572536317947197 1108 5 -0.45141359595233155 1108 6 -0.48433791900230005 1108 7 -0.4670223331309842 1108 8 -0.4958331409817376 1108 9 -0.5981363446545984 1108 10 -0.5864629720553296 1108 11 -0.5519851594522933 1108 12 -0.6936130809869923 1108 13 -0.6564297381010107 1108 14 -0.8919616569543223 1108 15 -0.9921557579445519 1108 16 -0.9042836331235122 1108 17 -0.9389820504118305 1108 18 -0.7533382337243779 1108 19 -0.8101900374536442 1108 20 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0.8687292937441455 1201 14 0.9643327071122343 1201 15 1.0591804974373176 1201 16 1.1208075192755373 1201 17 1.1068074845169917 1201 18 1.2363634845953158 1201 19 1.258988815189774 1201 20 1.0653380409424003 1201 21 1.1063928758478667 1201 22 1.039903076280062 1201 23 1.1492538995973334 1201 24 1.165886891137717 1201 25 1.0944335118728148 1202 1 2.6003548439345963 1202 2 1.8036698735921988 1202 3 1.4143200808487606 1202 4 1.1318204310128692 1202 5 0.9802882023901915 1202 6 0.9389540376050078 1202 7 0.8682802431312737 1202 8 0.7876744011697951 1202 9 0.9665921849584009 1202 10 1.0122466798385474 1202 11 1.0098872232372926 1202 12 1.0351938245173145 1202 13 1.0652855735614395 1202 14 1.1390262069793025 1202 15 1.1277860413256846 1202 16 1.2343540932552912 1202 17 1.1357532256289389 1202 18 1.3862408546688343 1202 19 1.310838421011482 1202 20 1.2555581922805743 1202 21 1.27458594404564 1202 22 1.256089106326634 1202 23 1.4037849334826262 1202 24 1.3800120363966784 1202 25 1.404198797297326 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-0.2621190667289426 2314 8 -0.23056026560840337 2314 9 -0.3394143745560285 2314 10 -0.2726530603632611 2314 11 -1.1559398375313208 2314 12 -0.85694826753695 2314 13 -0.7264403833220854 2314 14 -0.6042907192698124 2314 15 -0.6800094314077825 2314 16 -0.7574765529740546 2314 17 -0.7363780655651055 2314 18 -1.8566852291430536 2314 19 -1.3422700791725717 2314 20 -2.0514517784336306 2314 21 -1.4414314262867762 2314 22 -1.0867389598447303 2314 23 -0.8050427615958782 2314 24 -0.7756839900883321 2314 25 -0.8601368220078669 2315 1 -2.6182339288051715 2315 2 -1.2529662932053967 2315 3 -0.5562444738898726 2315 4 -0.3953020417313269 2315 5 -0.14172920590685578 2315 6 -0.18689473013095892 2315 7 -0.14780194281050285 2315 8 -0.16588313897646856 2315 9 -0.26628754561865264 2315 10 -0.26641039585995685 2315 11 -0.4112655359077806 2315 12 -0.49334227443034706 2315 13 -0.39030643517771324 2315 14 -0.5455665543896799 2315 15 -1.1640019023887191 2315 16 -0.9571477275735063 2315 17 -0.745459445321508 2315 18 -0.6691852872229944 2315 19 -0.6281079590037777 2315 20 -0.6839207428357383 2315 21 -0.7384378466759074 2315 22 -0.6661539879600724 2315 23 -0.7975667114408296 2315 24 -0.6856816854762329 2315 25 -0.5466401121204285 2316 1 1.2430859047674083 2316 2 0.5449285893700022 2316 3 -0.012929624395930261 2316 4 -0.21021857298747904 2316 5 -0.3276211300963965 2316 6 -0.5726456573590397 2316 7 -0.4106610842956755 2316 8 -0.4482308294681019 2316 9 -0.6154601448448105 2316 10 -0.6085918046637033 2316 11 -0.6779964466679971 2316 12 -0.5408600721088135 2316 13 -0.585474283498499 2316 14 -0.6540857583944302 2316 15 -0.54358066030386 2316 16 -0.5463128215350466 2316 17 -0.4722576119966746 2316 18 -0.3774676746258123 2316 19 -0.43242749382309853 2316 20 -0.3967536523280728 2316 21 -0.3908369810201435 2316 22 -0.5836423475132548 2316 23 -0.5616215945991598 2316 24 -0.5269850511866423 2316 25 -0.5276113516549614 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 40383.0 3 0.0 4 364.0 5 1718.0 6 2039.0 7 1771.0 8 1711.0 9 1712.0 10 1590.0 11 1617.0 12 1640.0 13 1680.0 14 1702.0 15 1702.0 16 1782.0 17 2016.0 18 2292.0 19 2669.0 20 3200.0 21 3896.0 22 4792.0 23 5637.0 24 6718.0 25 7908.0 26 9030.0 27 10067.0 28 11610.0 29 14301.0 30 17846.0 31 23493.0 32 31088.0 33 42498.0 34 59856.0 35 87903.0 36 152650.0 37 260401.0 38 812441.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.919115632804335 19.447368776649128 12.142359631030489 24.49115595951605 2 14.770107039458983 20.036870784047462 41.94723904988054 23.24578312661302 3 18.807081445713298 24.855980066241187 29.64065936630001 26.696279121745505 4 12.37891988897191 16.725739619358574 38.2040021439399 32.69133834772961 5 13.070886733346471 37.89766473355396 35.699171075870225 13.332277457229344 6 31.55741175517638 36.860323978561794 18.34514185567871 13.237122410583119 7 27.92065389524948 31.45716030286761 22.427892028163786 18.19429377371912 8 24.905438165156543 37.245573400861126 20.643646795581873 17.205341638400455 9 26.839023180799433 14.813998816780119 20.433004470214527 37.91397353220592 10 15.663840014038161 28.335612693983407 34.45620222315892 21.544345068819514 11 33.96286621245063 21.853854362456055 25.59239234153217 18.590887083561146 12 24.191547173145743 24.137548046949785 31.870959269931813 19.79994550997266 13 29.507199662203217 22.03258658804381 25.727238916349883 22.732974833403095 14 20.877277173586197 22.660416239655067 27.42856581788012 29.03374076887862 15 22.67318157807452 31.99359974448116 23.24257091261993 22.090647764824393 16 21.73765817144894 27.671691689354038 28.687983579053462 21.90266656014356 17 21.48858370586547 28.7962781412096 28.111491036179203 21.603647116745723 18 21.44857977508535 26.5962095060788 31.01422400401025 20.940986714825602 19 23.809917811241657 26.307573501020464 27.751362151494536 22.131146536243342 20 24.033131890978268 26.802034130312393 28.537251299235727 20.62758267947361 21 22.8178465289762 26.220797809904735 27.42316697881902 23.53818868230004 22 23.67291641762681 28.13099036637211 27.343993771704785 20.8520994442963 23 21.962404340198198 27.848623253639076 28.517681913938546 21.67129049222418 24 23.57454012073443 26.17257849335027 28.955932178403916 21.296949207511382 25 22.593679988103737 27.271012325166122 28.694569626926846 21.440738059803294 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 795.0 1 795.0 2 1158.0 3 1521.0 4 1521.0 5 1521.0 6 2464.5 7 3408.0 8 3408.0 9 3408.0 10 3976.5 11 4545.0 12 4545.0 13 4545.0 14 5868.5 15 7192.0 16 7192.0 17 7192.0 18 12719.5 19 18247.0 20 18247.0 21 18247.0 22 29184.0 23 40121.0 24 40121.0 25 40121.0 26 59790.0 27 79459.0 28 79459.0 29 79459.0 30 104144.0 31 128829.0 32 128829.0 33 128829.0 34 144045.5 35 159262.0 36 159262.0 37 159262.0 38 169216.5 39 179171.0 40 179171.0 41 179171.0 42 188655.0 43 198139.0 44 198139.0 45 198139.0 46 204755.5 47 211372.0 48 211372.0 49 211372.0 50 204049.0 51 196726.0 52 196726.0 53 196726.0 54 174284.5 55 151843.0 56 151843.0 57 151843.0 58 130936.5 59 110030.0 60 110030.0 61 110030.0 62 91292.0 63 72554.0 64 72554.0 65 72554.0 66 55973.0 67 39392.0 68 39392.0 69 39392.0 70 29148.5 71 18905.0 72 18905.0 73 18905.0 74 13286.0 75 7667.0 76 7667.0 77 7667.0 78 5332.0 79 2997.0 80 2997.0 81 2997.0 82 2001.0 83 1005.0 84 1005.0 85 1005.0 86 679.0 87 353.0 88 353.0 89 353.0 90 227.5 91 102.0 92 102.0 93 102.0 94 74.0 95 46.0 96 46.0 97 46.0 98 44.0 99 42.0 100 42.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3440607740724713 2 0.2932565679738854 3 0.31394550973451435 4 0.3027441004380792 5 0.2903797032911944 6 0.25151142513143293 7 0.35605791189816144 8 0.31578180634048736 9 0.261427426803687 10 0.2374943610391725 11 0.2510829559233726 12 0.24894060988307076 13 0.2657121188842907 14 0.25591853698576805 15 0.3466315893208335 16 0.39988419089405 17 0.4158599713660149 18 0.11654362459241865 19 0.1293364909473638 20 0.19274993374029747 21 0.19030153826566681 22 0.1504539019160531 23 0.2521847338869564 24 0.34810062660561186 25 0.38690769487850757 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1633723.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.68642497547903 #Duplication Level Percentage of deduplicated Percentage of total 1 84.07544393511247 57.748416721291974 2 10.606635308042108 14.570637206562026 3 2.726384180048141 5.617967473116288 4 0.9948983996926367 2.733440571548498 5 0.4710965914892221 1.6178970343764174 6 0.28205998934488025 1.162421537803292 7 0.16175672276879477 0.7777343701916727 8 0.1274225077904149 0.700175721722698 9 0.084327437056178 0.5212935160860241 >10 0.4124200318054878 5.044992701597349 >50 0.02881807400434203 1.3866781399439854 >100 0.024171487888804445 3.4730467812548294 >500 0.002685491150857797 1.4096802476899977 >1k 0.0017903274339051982 2.8637530953203356 >5k 8.951637169525991E-5 0.3718648814947052 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6048 0.3701973957641534 No Hit GTCCTACAGTGGACATTTCTAAATT 4822 0.2951540744667242 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4539 0.2778316764837124 No Hit CTGTAGGACGTGGAATATGGCAAGA 4356 0.26663026718727717 No Hit CTTTAGGACGTGAAATATGGCGAGG 3836 0.23480112601707878 No Hit GGTATCAACGCAGAGTACTTTTTTT 3744 0.2291698164254283 No Hit TATCAACGCAGAGTACTTTTTTTTT 3586 0.21949865430063725 No Hit GTCCTACAGTGTGCATTTCTCATTT 2251 0.1377834553348395 No Hit CTGAAGGACCTGGAATATGGCGAGA 2128 0.13025463925035027 No Hit GTCCTTCAGTGTGCATTTCTCATTT 1869 0.11440127855211685 No Hit ATTTAGAAATGTCCACTGTAGGACG 1756 0.10748456133628527 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1668 0.10209809129209788 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1668 0.10209809129209788 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 6.12098868657661E-5 0.0 3 0.0 0.0 0.0 1.224197737315322E-4 0.0 4 0.0 0.0 0.0 1.836296605972983E-4 0.0 5 0.0 0.0 0.0 1.836296605972983E-4 0.0 6 0.0 0.0 0.0 1.836296605972983E-4 0.0 7 0.0 0.0 0.0 1.836296605972983E-4 0.0 8 0.0 0.0 0.0 1.836296605972983E-4 0.0 9 0.0 6.12098868657661E-5 0.0 1.836296605972983E-4 0.0 10 0.0 6.12098868657661E-5 0.0 1.836296605972983E-4 0.0 11 0.0 6.12098868657661E-5 0.0 1.836296605972983E-4 0.0 12 0.0 6.12098868657661E-5 0.0 1.836296605972983E-4 0.0 13 0.0 6.12098868657661E-5 0.0 1.836296605972983E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGCTCG 40 0.0053045875 14.239716 11 GGTATCA 1035 0.0 13.862261 1 AAGGCGT 85 3.9361366E-6 12.296208 6 TAGGACC 650 0.0 12.279389 4 CGTGCGC 80 2.8941742E-5 11.863876 10 TATCGCG 110 4.9552364E-7 11.229528 7 GCGTGCG 95 1.3866167E-5 10.98092 9 CGCGCCT 115 8.883835E-7 10.732698 12 GCATAAG 125 2.234392E-7 10.641809 1 TCGCGCG 125 2.2938366E-7 10.621544 9 GTCCTAC 2330 0.0 10.521103 1 GTATCAA 2365 0.0 10.485927 1 GGCGAGG 1080 0.0 10.384272 19 TAGACTG 165 9.258656E-10 10.365718 5 CTACACT 295 0.0 10.307155 4 ATCGCGC 120 1.5626156E-6 10.271291 8 TGGTATC 300 0.0 10.135681 2 GTCCTAT 235 0.0 10.108102 1 TCTACAC 245 0.0 10.083968 3 GTCCTAA 1425 0.0 10.068378 1 >>END_MODULE