FastQCFastQC Report
Thu 26 May 2016
SRR1512271_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512271_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1633723
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69010.4224094292606519No Hit
GTCCTACAGTGGACATTTCTAAATT48740.29833698858374397No Hit
CTGTAGGACGTGGAATATGGCAAGA47650.2916651109153755No Hit
GTCCTAAAGTGTGTATTTCTCATTT45720.27985160275028265No Hit
GGTATCAACGCAGAGTACTTTTTTT43940.26895624288817627No Hit
TATCAACGCAGAGTACTTTTTTTTT43770.2679156748114582No Hit
CTTTAGGACGTGAAATATGGCGAGG41890.2564082160806942No Hit
CTGAAGGACCTGGAATATGGCGAGA23670.14488380221126837No Hit
GTCCTACAGTGTGCATTTCTCATTT23600.144455333003208No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21190.12970375026855838No Hit
GTCCTTCAGTGTGCATTTCTCATTT18750.11476853787331144No Hit
ATTTAGAAATGTCCACTGTAGGACG17870.10938206782912403No Hit
TTTCTAAATTTTCCACCTTTTTCAG17650.10803545031807718No Hit
CTGTAGGACCTGGAATATGGCGAGA17600.10772940088374834No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17200.1052810054091177No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6900.013.9077694
GGTATCA10650.013.0408991
AAGGCGT1103.7951395E-812.0952186
GGCGAGG11650.011.32839119
AGGACCT14950.011.3133345
GGACCTG14800.011.1728986
CTACACT1803.6379788E-1211.0849054
GTTAGAC951.3580639E-511.001563
AAATGTC6550.010.881747
GTATCAA23050.010.8127131
TTAGACT1252.2352833E-710.641514
GTCTAGG1002.3634404E-510.4639821
ATTTAGA7650.010.4453311
GTCCTAA12650.010.3775021
ACCTTTT6500.010.371407515
TTAGGAC15500.010.1756373
AATGTCC7100.010.1726388
GCCTAAC750.00261848510.1468921
GGACGTG25150.010.1268676
AGGACGT25600.010.0586835
Produced by FastQC (version 0.11.5)