FastQCFastQC Report
Thu 26 May 2016
SRR1512270_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512270_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1954795
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT70680.3615724410999619No Hit
GTCCTACAGTGGACATTTCTAAATT62530.3198800897280789No Hit
GTCCTAAAGTGTGTATTTCTCATTT57820.29578549157328515No Hit
CTGTAGGACGTGGAATATGGCAAGA56190.2874470212989086No Hit
CTTTAGGACGTGAAATATGGCGAGG48180.24647085755795367No Hit
GGTATCAACGCAGAGTACTTTTTTT43260.22130197795676784No Hit
TATCAACGCAGAGTACTTTTTTTTT40130.2052900687795907No Hit
GTCCTACAGTGTGCATTTCTCATTT31040.15878902902861938No Hit
CTGAAGGACCTGGAATATGGCGAGA26770.13694530628531382No Hit
ATTTAGAAATGTCCACTGTAGGACG22980.11755708399090442No Hit
GTCCTTCAGTGTGCATTTCTCATTT22590.11556198987617627No Hit
CTGTAGGACCTGGAATATGGCGAGA20320.10394951900327144No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19910.10185211236983929No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCAA350.002182045516.27274511
GGTATCA12500.013.6824621
TAGGACC9350.013.6177744
TGTACGC500.00149746113.3027352
AATCCCG2050.012.51863219
ACTGTTC5500.012.2375228
TAGACAG3450.011.8427115
TCCAACG1459.640644E-1111.79431618
GGCGTGC1451.0186341E-1011.7679918
AGGCGTG1701.8189894E-1211.7371047
CGTGCGC907.5555054E-611.59828910
TAGAAAT7650.011.5513964
GGCGAGG13650.011.48942519
AGGACCT18200.011.4855835
CTACACG752.070367E-411.4023464
ATTAGAC1001.9254403E-611.4023453
AAATGTC7450.011.3507447
GGACCTG18200.011.0676416
GTGGTAT3800.011.001981
GTATTAT2600.010.9635111
Produced by FastQC (version 0.11.5)