##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512270_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1954795 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.960600983734867 31.0 30.0 34.0 16.0 34.0 2 29.83190002020672 31.0 30.0 34.0 22.0 34.0 3 30.47884560785146 33.0 31.0 34.0 26.0 34.0 4 34.12878588291867 37.0 35.0 37.0 30.0 37.0 5 34.29683828739075 37.0 35.0 37.0 31.0 37.0 6 34.44754718525472 37.0 35.0 37.0 32.0 37.0 7 34.38785243465427 37.0 35.0 37.0 32.0 37.0 8 34.38493141224527 37.0 35.0 37.0 32.0 37.0 9 35.95891487342663 39.0 37.0 39.0 31.0 39.0 10 35.8781872267936 39.0 37.0 39.0 31.0 39.0 11 35.90484628822971 39.0 37.0 39.0 31.0 39.0 12 35.8418800948437 39.0 37.0 39.0 31.0 39.0 13 35.81626359797319 39.0 37.0 39.0 31.0 39.0 14 37.03745252059679 40.0 38.0 41.0 31.0 41.0 15 36.969603973818224 40.0 38.0 41.0 31.0 41.0 16 36.89654158108651 40.0 38.0 41.0 31.0 41.0 17 36.864151483915194 40.0 38.0 41.0 31.0 41.0 18 36.639223038732965 40.0 38.0 41.0 30.0 41.0 19 36.753886213132326 40.0 38.0 41.0 30.0 41.0 20 36.68636097391286 40.0 38.0 41.0 30.0 41.0 21 36.63011927081868 40.0 38.0 41.0 30.0 41.0 22 36.55294135702209 40.0 37.0 41.0 30.0 41.0 23 36.43032696523165 40.0 37.0 41.0 30.0 41.0 24 36.307885481597815 40.0 37.0 41.0 30.0 41.0 25 36.19792714837106 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1006832239812603 1101 2 0.27848372636524843 1101 3 -0.12606971217858387 1101 4 -0.24586475103430416 1101 5 -0.5133334192880383 1101 6 -0.38505725398841406 1101 7 -0.3658713136131979 1101 8 -0.501203416347316 1101 9 -0.6451167581702322 1101 10 -0.8722976154120587 1101 11 -0.8743115456105741 1101 12 -0.9284606017194648 1101 13 -0.8753313301875707 1101 14 -1.2054379438075458 1101 15 -1.3807494322449045 1101 16 -1.325008388806502 1101 17 -1.3147448569452678 1101 18 -0.9890722707012145 1101 19 -1.1362973198687385 1101 20 -1.257289157650625 1101 21 -1.318656429477599 1101 22 -1.6184732771536972 1101 23 -1.8299379977903456 1101 24 -1.8449467670183708 1101 25 -1.9723372607357277 1102 1 1.099578409086213 1102 2 0.3306696978384487 1102 3 -0.17361551810057563 1102 4 -0.1331402820637564 1102 5 -0.4283952159519302 1102 6 -0.5220371985766477 1102 7 -0.5078197897895578 1102 8 -0.594322836690047 1102 9 -0.4887301527681558 1102 10 -0.7626689654753349 1102 11 -0.7601690028260819 1102 12 -0.8705647097154099 1102 13 -0.8941217346022654 1102 14 -1.2267093360567358 1102 15 -1.2089145159896475 1102 16 -1.2667547716493743 1102 17 -1.3598162309518997 1102 18 -1.3628699721389594 1102 19 -1.417817323973317 1102 20 -1.5860924458716354 1102 21 -1.4978929784417616 1102 22 -1.7700173386311207 1102 23 -1.7351949898206271 1102 24 -1.6968169142130023 1102 25 -1.9303397576858004 1103 1 1.2631605363251417 1103 2 0.4318927151696741 1103 3 -0.13586583775588323 1103 4 -0.023914331424983004 1103 5 -0.15308039576475352 1103 6 -0.3749331682335679 1103 7 -0.32176965494336685 1103 8 -0.2906776916900995 1103 9 -0.5962125045716533 1103 10 -0.6378465219119605 1103 11 -0.5314134558662928 1103 12 -0.7108028663518837 1103 13 -0.756877055923006 1103 14 -1.034626848869813 1103 15 -1.1349814663168232 1103 16 -1.0301856670065845 1103 17 -1.0998972461126968 1103 18 -1.0294630374990703 1103 19 -1.1816479227763566 1103 20 -1.2560851394645596 1103 21 -1.3431411276643814 1103 22 -1.4530285990937841 1103 23 -1.2833474358552905 1103 24 -1.2957010754267273 1103 25 -1.459969118697522 1104 1 1.346250231628023 1104 2 0.6844135194743366 1104 3 0.12180660523636533 1104 4 0.12146481092858608 1104 5 -0.09382970566827709 1104 6 -0.052864227544347386 1104 7 -0.17185189862399142 1104 8 -0.19367324789467943 1104 9 -0.33920531255953534 1104 10 -0.4033506219267622 1104 11 -0.3410923505761332 1104 12 -0.3227701331641697 1104 13 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-1.2377485778273893 1108 22 -1.3098975531849604 1108 23 -1.2610076357377125 1108 24 -1.3056283909095967 1108 25 -1.2666804115199284 1109 1 0.684846057926169 1109 2 0.07776810048316918 1109 3 -0.40280398418283525 1109 4 -0.38831332061273116 1109 5 -0.4258373627021186 1109 6 -0.4491813179464401 1109 7 -0.5175910572497671 1109 8 -0.568934666451085 1109 9 -0.6581404020508828 1109 10 -0.5717721035984198 1109 11 -0.6439954083299568 1109 12 -0.7430890075839471 1109 13 -0.6216867853820816 1109 14 -1.0065674206202715 1109 15 -0.9271820953362067 1109 16 -1.1033013471142326 1109 17 -1.0941253673475728 1109 18 -1.070470563825566 1109 19 -1.2350715407331379 1109 20 -1.4305108854471271 1109 21 -1.3805908608787973 1109 22 -1.4380267996602143 1109 23 -1.4445004033559528 1109 24 -1.4149174612252864 1109 25 -1.6647094526098059 1110 1 0.301780176545158 1110 2 -0.15965229030874184 1110 3 -0.4861625701269219 1110 4 -0.4037674951655461 1110 5 -0.3516276988806766 1110 6 -0.4920966486230327 1110 7 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-0.5953749983098362 2314 14 -0.5660523441325154 2314 15 -0.6600360169915263 2314 16 -1.0133429069338504 2314 17 -0.7485028543048671 2314 18 -1.8038864272971367 2314 19 -1.225970824489437 2314 20 -1.9523709084376648 2314 21 -1.1826109333874157 2314 22 -0.8341851691004862 2314 23 -0.6791077956983358 2314 24 -0.6364198044917089 2314 25 -0.5508510212719315 2315 1 -2.2726483059487954 2315 2 -0.8963725166304641 2315 3 -0.27621308273931433 2315 4 -0.12605971181028508 2315 5 -0.018606634884896778 2315 6 0.004931539148344655 2315 7 0.04700633835573598 2315 8 0.041470966309098856 2315 9 0.05366130729330365 2315 10 0.0525143797960439 2315 11 -0.08018471483421052 2315 12 -0.11092978284555954 2315 13 -0.08015375937178959 2315 14 -0.06380908420248943 2315 15 -0.6416454016385771 2315 16 -0.4323427011301817 2315 17 -0.40630898725403597 2315 18 -0.1708137786039856 2315 19 -0.11755345466025346 2315 20 -0.17061334515304338 2315 21 -0.15209848226578515 2315 22 0.08727603690898178 2315 23 -0.20959966645363437 2315 24 -0.01100716814678293 2315 25 0.0978503576126144 2316 1 1.1237862665359621 2316 2 0.4057824226388469 2316 3 -0.21583573632634412 2316 4 -0.3322054334572897 2316 5 -0.47786570448114674 2316 6 -0.6711087239379552 2316 7 -0.49155436832864297 2316 8 -0.6506271629616549 2316 9 -0.5566086602209737 2316 10 -0.6758621344704707 2316 11 -0.7970379209984984 2316 12 -0.6796129110761342 2316 13 -0.6861887415286461 2316 14 -0.7969518773340809 2316 15 -0.8523519259868593 2316 16 -0.7705673486681519 2316 17 -0.747380855902918 2316 18 -0.5468991696328089 2316 19 -0.8366323325427629 2316 20 -0.7426295400033354 2316 21 -0.6983424612060816 2316 22 -0.8635985170125053 2316 23 -0.7110645642756523 2316 24 -0.7878088452419547 2316 25 -0.8284573125373313 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 58000.0 3 5.0 4 462.0 5 2329.0 6 2766.0 7 2530.0 8 2385.0 9 2295.0 10 2285.0 11 2203.0 12 2266.0 13 2308.0 14 2247.0 15 2266.0 16 2431.0 17 2584.0 18 2829.0 19 3517.0 20 4074.0 21 5098.0 22 6296.0 23 7646.0 24 8775.0 25 10365.0 26 11575.0 27 13052.0 28 14853.0 29 17665.0 30 23123.0 31 29442.0 32 39237.0 33 52955.0 34 74939.0 35 107691.0 36 185254.0 37 314798.0 38 932249.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.866301156081235 19.50630403088359 12.020421363888376 24.6069734491468 2 14.337529971404983 19.961558610547986 42.84952699953462 22.851384418512414 3 18.94694273033703 24.92579043959157 29.635428855304703 26.491837974766696 4 11.973200966579721 16.76910105930692 38.91786792095558 32.33983005315778 5 12.653169911049474 38.2622709089146 36.169160431612966 12.91539874842296 6 31.282245640235583 37.48135778890555 18.355013344438312 12.881383226420551 7 27.615268777786163 31.55553228008402 22.65127815711662 18.1779207850132 8 24.318967499302033 38.17543848844657 20.70261820301851 16.802975809232894 9 26.66147936560368 14.842844781556325 20.480367330671978 38.01530852216802 10 15.135893783273492 28.71383695207387 35.09660401430472 21.053665250347926 11 34.18180046465589 21.712764702563813 25.797120774631633 18.30831405814866 12 23.95122701746288 24.196938226017387 32.50942380183091 19.34241095468882 13 29.53102790996138 22.147565630291368 25.81041785178806 22.510988607959195 14 20.36749149230268 22.96342267163494 27.934738845107336 28.73434699095504 15 22.414910110167664 32.51398913724242 23.355955522244013 21.715145230345904 16 21.20647101577689 27.87401089460586 29.459712815929446 21.459805273687806 17 21.039144254693245 29.04791090730025 28.613431411886253 21.29951342612025 18 20.901949797665406 26.973575373413734 31.61782142416307 20.506653404757795 19 23.49714375868244 26.50910554979572 27.904647846344872 22.08910284517697 20 23.601074751840372 27.37311131636598 28.872832992134136 20.15298093965951 21 22.440587304857885 26.602833193406095 27.644752933602735 23.31182656813329 22 23.07989062857828 28.737612590111794 27.738576275910265 20.443920505399664 23 21.57346751317172 28.26972322074693 28.796643384187576 21.360165881893774 24 23.210996457723702 26.539247394630117 29.49057959854202 20.759176549104165 25 22.33701242908141 27.536724620427943 29.03570234504478 21.09056060544587 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 917.0 1 917.0 2 1386.0 3 1855.0 4 1855.0 5 1855.0 6 3018.0 7 4181.0 8 4181.0 9 4181.0 10 4910.0 11 5639.0 12 5639.0 13 5639.0 14 7525.0 15 9411.0 16 9411.0 17 9411.0 18 16595.5 19 23780.0 20 23780.0 21 23780.0 22 38126.5 23 52473.0 24 52473.0 25 52473.0 26 77278.5 27 102084.0 28 102084.0 29 102084.0 30 134034.0 31 165984.0 32 165984.0 33 165984.0 34 183118.0 35 200252.0 36 200252.0 37 200252.0 38 210299.0 39 220346.0 40 220346.0 41 220346.0 42 229723.0 43 239100.0 44 239100.0 45 239100.0 46 245243.0 47 251386.0 48 251386.0 49 251386.0 50 239442.5 51 227499.0 52 227499.0 53 227499.0 54 199068.0 55 170637.0 56 170637.0 57 170637.0 58 146843.5 59 123050.0 60 123050.0 61 123050.0 62 101383.5 63 79717.0 64 79717.0 65 79717.0 66 61632.5 67 43548.0 68 43548.0 69 43548.0 70 31854.5 71 20161.0 72 20161.0 73 20161.0 74 14136.0 75 8111.0 76 8111.0 77 8111.0 78 5573.0 79 3035.0 80 3035.0 81 3035.0 82 2035.0 83 1035.0 84 1035.0 85 1035.0 86 697.0 87 359.0 88 359.0 89 359.0 90 246.0 91 133.0 92 133.0 93 133.0 94 98.5 95 64.0 96 64.0 97 64.0 98 51.0 99 38.0 100 38.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.349653032670945 2 0.29946874224662945 3 0.3201870272841909 4 0.3104161817479582 5 0.293329991124389 6 0.251637639752506 7 0.363618691474042 8 0.321414777508639 9 0.2640686107750429 10 0.23884857491450512 11 0.2539908276826982 12 0.25296770249565814 13 0.26990042434117134 14 0.26038536010169866 15 0.3506761578579851 16 0.40720382444194914 17 0.4260293278834865 18 0.11950102184628056 19 0.13193199286881746 20 0.19608194209623006 21 0.19342181660992583 22 0.1490693397517387 23 0.2544512340168662 24 0.35272240823206524 25 0.3922661967111641 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1954795.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.93819597847971 #Duplication Level Percentage of deduplicated Percentage of total 1 83.94427461358956 57.03022579969365 2 10.838177692238311 14.726524802097465 3 2.717867098421103 5.539409627279848 4 0.9833750663511167 2.6723491191245055 5 0.44656384027780055 1.5169370848847863 6 0.24915309648937245 1.0156207138764028 7 0.14824311879214372 0.7049959039873196 8 0.1123701497498076 0.6107380204666832 9 0.07918198185841417 0.484153290131523 >10 0.4179922880082405 5.183902173031642 >50 0.031848471651326246 1.503752289710828 >100 0.02558090845934892 3.5468238943164456 >500 0.0027993231295684695 1.3450420134662218 >1k 0.002269721456406862 2.8488080309672785 >5k 3.026295275209149E-4 1.2707172369654494 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7068 0.3615724410999619 No Hit GTCCTACAGTGGACATTTCTAAATT 6253 0.3198800897280789 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5782 0.29578549157328515 No Hit CTGTAGGACGTGGAATATGGCAAGA 5619 0.2874470212989086 No Hit CTTTAGGACGTGAAATATGGCGAGG 4818 0.24647085755795367 No Hit GGTATCAACGCAGAGTACTTTTTTT 4326 0.22130197795676784 No Hit TATCAACGCAGAGTACTTTTTTTTT 4013 0.2052900687795907 No Hit GTCCTACAGTGTGCATTTCTCATTT 3104 0.15878902902861938 No Hit CTGAAGGACCTGGAATATGGCGAGA 2677 0.13694530628531382 No Hit ATTTAGAAATGTCCACTGTAGGACG 2298 0.11755708399090442 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2259 0.11556198987617627 No Hit CTGTAGGACCTGGAATATGGCGAGA 2032 0.10394951900327144 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1991 0.10185211236983929 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 5.115625935200366E-5 12 0.0 0.0 0.0 1.0231251870400732E-4 1.0231251870400732E-4 13 0.0 0.0 0.0 1.0231251870400732E-4 1.0231251870400732E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTCAA 35 0.0021820455 16.272745 11 GGTATCA 1250 0.0 13.682462 1 TAGGACC 935 0.0 13.617774 4 TGTACGC 50 0.001497461 13.302735 2 AATCCCG 205 0.0 12.518632 19 ACTGTTC 550 0.0 12.237522 8 TAGACAG 345 0.0 11.842711 5 TCCAACG 145 9.640644E-11 11.794316 18 GGCGTGC 145 1.0186341E-10 11.767991 8 AGGCGTG 170 1.8189894E-12 11.737104 7 CGTGCGC 90 7.5555054E-6 11.598289 10 TAGAAAT 765 0.0 11.551396 4 GGCGAGG 1365 0.0 11.489425 19 AGGACCT 1820 0.0 11.485583 5 CTACACG 75 2.070367E-4 11.402346 4 ATTAGAC 100 1.9254403E-6 11.402345 3 AAATGTC 745 0.0 11.350744 7 GGACCTG 1820 0.0 11.067641 6 GTGGTAT 380 0.0 11.00198 1 GTATTAT 260 0.0 10.963511 1 >>END_MODULE