FastQCFastQC Report
Thu 26 May 2016
SRR1512270_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512270_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1954795
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT77800.3979956977585885No Hit
GTCCTACAGTGGACATTTCTAAATT64260.32873012259597556No Hit
CTGTAGGACGTGGAATATGGCAAGA62950.32202865262086305No Hit
GTCCTAAAGTGTGTATTTCTCATTT58750.30054302369302155No Hit
CTTTAGGACGTGAAATATGGCGAGG52260.2673426113735711No Hit
GGTATCAACGCAGAGTACTTTTTTT51690.26442670459050693No Hit
TATCAACGCAGAGTACTTTTTTTTT50480.25823679720891446No Hit
GTCCTACAGTGTGCATTTCTCATTT32680.16717865556234798No Hit
CTGAAGGACCTGGAATATGGCGAGA29540.15111559012581882No Hit
GTCCTTCAGTGTGCATTTCTCATTT24450.12507705411564896No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24120.12338889755703283No Hit
ATTTAGAAATGTCCACTGTAGGACG23590.12067761581137663No Hit
TTTCTAAATTTTCCACCTTTTTCAG22130.11320880194598411No Hit
CTGTAGGACCTGGAATATGGCGAGA21380.10937208249458383No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19970.10215904992595132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13450.014.5660991
TAGGACC8650.013.5116744
AATCGGA500.001505674713.29308818
TCCAACG1600.013.05571118
ACACCGT1155.298716E-912.3956466
ATCGCGC802.8562357E-511.8794658
TTTAGAA7950.011.4742492
CCAACGA1105.003585E-711.22143719
TAGAAAT7300.011.1942694
GAATTAC1201.2565943E-711.0954551
CAGAACG600.00586685911.0857974
TAGCACG600.00586685911.0857974
TGTAGGA30800.011.0446632
AATGTCC6900.011.0186348
TGGCGAA951.3684421E-510.99428318
ATAGGAC2600.010.9639763
CTGTAGG30350.010.7481511
GTCCTAC27400.010.6210921
ATGTCCA7800.010.5987819
GTAGCAC1355.6701538E-810.5579023
Produced by FastQC (version 0.11.5)