FastQCFastQC Report
Thu 26 May 2016
SRR1512269_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512269_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2011902
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64920.32267973290945584No Hit
GGTATCAACGCAGAGTACTTTTTTT40100.19931388308178033No Hit
TATCAACGCAGAGTACTTTTTTTTT37590.18683812631032723No Hit
GTCCTAAAGTGTGTATTTCTCATTT27430.13633864870157691No Hit
GTCCTACAGTGGACATTTCTAAATT27380.13609012765035275No Hit
CTGTAGGACGTGGAATATGGCAAGA25840.12843567927264846No Hit
CTTTAGGACGTGAAATATGGCGAGG23990.11924040037735435No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACCG400.00526095414.25768519
GGTATCA12800.013.2104691
TAGGACC5850.011.6942294
GTATCAA27850.010.7787911
ATACCGA803.7694527E-410.6881896
GTCCTAG2050.010.6581581
GTAGGAC16600.010.36039453
ATCGCGC1106.180526E-610.3446658
GTCTTAT1752.3646862E-1010.313921
TGTAGGA16950.010.1462112
CCCGCAC750.002643985410.1352523
GTCCTAC15000.010.0063111
GTCCTAA8200.09.963061
TCCAACG1054.09391E-59.9550118
AGGACGT15950.09.9480945
TATCGCG1151.0258269E-59.9136827
TCGCGCG1151.0473477E-59.8963789
CTGTAGG16100.09.7946831
GTGGTAT3900.09.7432441
CTTACAC2054.1836756E-119.733553
Produced by FastQC (version 0.11.5)