##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512269_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2011902 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.916977069459644 31.0 30.0 34.0 16.0 34.0 2 29.779472857027827 31.0 30.0 34.0 22.0 34.0 3 30.41600386102305 33.0 31.0 34.0 26.0 34.0 4 34.06567268187019 37.0 35.0 37.0 30.0 37.0 5 34.22899723744 37.0 35.0 37.0 30.0 37.0 6 34.375305059590374 37.0 35.0 37.0 31.0 37.0 7 34.31167074738233 37.0 35.0 37.0 31.0 37.0 8 34.312207552852975 37.0 35.0 37.0 31.0 37.0 9 35.870699467469095 39.0 37.0 39.0 31.0 39.0 10 35.80269615518052 39.0 37.0 39.0 31.0 39.0 11 35.82351824293629 39.0 37.0 39.0 31.0 39.0 12 35.75216884321404 39.0 37.0 39.0 30.0 39.0 13 35.724271361129915 39.0 37.0 39.0 30.0 39.0 14 36.945071877258435 40.0 38.0 41.0 31.0 41.0 15 36.86164435444669 40.0 38.0 41.0 31.0 41.0 16 36.78106687105038 40.0 38.0 41.0 30.0 41.0 17 36.73777798322185 40.0 38.0 41.0 30.0 41.0 18 36.50982254602858 40.0 37.0 41.0 29.0 41.0 19 36.61777909659616 40.0 37.0 41.0 30.0 41.0 20 36.53915200640986 40.0 37.0 41.0 30.0 41.0 21 36.48536608641972 40.0 37.0 41.0 30.0 41.0 22 36.40140722560045 40.0 37.0 41.0 30.0 41.0 23 36.27636932614014 40.0 37.0 41.0 29.0 41.0 24 36.13458359303783 40.0 37.0 41.0 29.0 41.0 25 36.008805597886976 40.0 37.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9072785301348674 1101 2 0.08513604642922701 1101 3 -0.435933200655203 1101 4 -0.5976499483347268 1101 5 -0.7004408507269986 1101 6 -0.8755604034944469 1101 7 -0.8119962038988859 1101 8 -0.884676152557546 1101 9 -0.9663097683829918 1101 10 -0.9807370763583023 1101 11 -1.066571120674709 1101 12 -1.2603109477392138 1101 13 -1.0357803665890728 1101 14 -1.5200280248698874 1101 15 -1.7355640674573252 1101 16 -1.763571290640499 1101 17 -1.6850442796079577 1101 18 -1.7926318108581825 1101 19 -1.6947169236273183 1101 20 -2.061419730572709 1101 21 -2.0168183600413983 1101 22 -2.078546692920007 1101 23 -2.0002924438201717 1101 24 -2.204160655097674 1101 25 -2.105981559025146 1102 1 0.8567050172834278 1102 2 0.051168558943434306 1102 3 -0.4738711717746362 1102 4 -0.50590225898641 1102 5 -0.6466906897526243 1102 6 -0.6452099221124854 1102 7 -0.6163020366699499 1102 8 -0.7818662256170583 1102 9 -0.885667939703616 1102 10 -0.9245437618690957 1102 11 -0.9681227177625686 1102 12 -1.1624714393895772 1102 13 -1.0591351692677904 1102 14 -1.238785869513741 1102 15 -1.424571496179965 1102 16 -1.3669022129498885 1102 17 -1.6763133950704017 1102 18 -1.716029165627944 1102 19 -1.5583501407978844 1102 20 -1.7145428685922042 1102 21 -1.717157601297643 1102 22 -1.8383614280639335 1102 23 -1.8630103600267702 1102 24 -1.877260027682894 1102 25 -2.132046216783827 1103 1 1.0606214807961152 1103 2 0.1945952278929255 1103 3 -0.33157435197789553 1103 4 -0.34852269632227717 1103 5 -0.44094515903695 1103 6 -0.44908198453565973 1103 7 -0.5474530927939725 1103 8 -0.6584985912857988 1103 9 -0.8015130086429991 1103 10 -0.68381304679599 1103 11 -0.6429779000907416 1103 12 -0.9681777722684117 1103 13 -0.7613773004011577 1103 14 -1.1837426416338204 1103 15 -1.128405327084657 1103 16 -1.2655046936129892 1103 17 -1.4103949726569738 1103 18 -1.281366037833827 1103 19 -1.463830330404413 1103 20 -1.4153895369123504 1103 21 -1.2802773923151989 1103 22 -1.5340415142496155 1103 23 -1.4191949585475072 1103 24 -1.3821088397431396 1103 25 -1.5185824783756203 1104 1 1.0965445326540468 1104 2 0.445271420552924 1104 3 -0.15393541448015924 1104 4 -0.08892264322130217 1104 5 -0.39242196959921927 1104 6 -0.3234243168753608 1104 7 -0.4556390910319834 1104 8 -0.4445028635007162 1104 9 -0.5111539736325597 1104 10 -0.44106024390922727 1104 11 -0.5103333507852739 1104 12 -0.5888458790979172 1104 13 -0.5787746284565713 1104 14 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-0.5015460526912889 2314 15 -0.5755099074481649 2314 16 -0.6520206711026546 2314 17 -0.6598882553069032 2314 18 -1.7891671702158405 2314 19 -1.2403302081147416 2314 20 -1.7365601482771353 2314 21 -1.2029447027622595 2314 22 -0.8407209692012856 2314 23 -0.7669644198248164 2314 24 -0.6450618902211076 2314 25 -0.6754590082668059 2315 1 -2.5869060324022897 2315 2 -1.2354711515869354 2315 3 -0.5659306921319889 2315 4 -0.4403296805466681 2315 5 -0.3047170137001345 2315 6 -0.2938730255013482 2315 7 -0.225551231402072 2315 8 -0.1838831408531192 2315 9 -0.26989194319394727 2315 10 -0.27165404497175416 2315 11 -0.4585574227839757 2315 12 -0.28915146293243055 2315 13 -0.20600717843903738 2315 14 -0.40926730193947236 2315 15 -0.8821410493873572 2315 16 -0.7079312942779481 2315 17 -0.6391093954547031 2315 18 -0.3429163571658691 2315 19 -0.37562252429420795 2315 20 -0.42939575121322804 2315 21 -0.48430938122990597 2315 22 -0.48649532656162364 2315 23 -0.593249879249484 2315 24 -0.27904492179010276 2315 25 -0.10796221284034857 2316 1 0.9612073223424424 2316 2 0.2406034229832663 2316 3 -0.2955932566813573 2316 4 -0.4656277903439445 2316 5 -0.7315227421016104 2316 6 -0.8323315297909062 2316 7 -0.7037599138772208 2316 8 -0.7719310381383337 2316 9 -0.8956813584081829 2316 10 -1.0371751644890352 2316 11 -1.035075994652999 2316 12 -1.087476206326457 2316 13 -1.0486678668907388 2316 14 -1.0961844228597712 2316 15 -1.0688977720706632 2316 16 -0.9626091185039485 2316 17 -1.030444770068847 2316 18 -0.8490325853482759 2316 19 -0.9478675129415066 2316 20 -0.9764829317118 2316 21 -0.9521950328898754 2316 22 -1.10841134279368 2316 23 -0.972231843497525 2316 24 -1.1587169105160413 2316 25 -1.2730796530592343 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 61143.0 3 4.0 4 505.0 5 2489.0 6 2981.0 7 2733.0 8 2500.0 9 2444.0 10 2428.0 11 2519.0 12 2403.0 13 2418.0 14 2491.0 15 2443.0 16 2645.0 17 2749.0 18 3101.0 19 3707.0 20 4624.0 21 5650.0 22 6862.0 23 8092.0 24 9678.0 25 11198.0 26 12680.0 27 14017.0 28 16285.0 29 19474.0 30 24677.0 31 31677.0 32 41875.0 33 56237.0 34 77702.0 35 111989.0 36 189000.0 37 319704.0 38 948778.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.79109151931672 18.83979468517712 11.893236691121496 24.47587710438466 2 15.282744964709845 19.87335625855583 40.66513388722107 24.178764889513257 3 18.91543268909549 24.600338855609415 29.32163061547467 27.162597839820418 4 12.630366144462565 16.44860661095138 37.69900483250266 33.222022412083405 5 13.60385109459797 37.50295975382824 35.15340123694032 13.739787914633473 6 32.245462943677815 36.309605076041976 18.03242583902282 13.41250614125739 7 28.311806995245114 31.354742611773673 22.04984745664636 18.28360293633485 8 25.708004623133817 35.992283475088826 20.382126541834076 17.917585359943278 9 26.913675729468416 14.942285871188583 19.753864676401715 38.390173722941284 10 15.665797125132276 28.31712824110148 33.92861057359849 22.088464060167755 11 34.7518020275987 21.992061411944555 24.302284212613845 18.953852347842897 12 23.70133785096789 24.230997284436583 31.221156481227734 20.846508383367794 13 29.302024018714224 21.573186910010055 25.598898037577953 23.525891033697764 14 21.588061385320604 21.57560373510252 27.27966278635778 29.5566720932191 15 23.55126967152935 30.020479538417337 23.317096673795767 23.11115411625754 16 22.936733068365335 27.36370151375112 26.988308004335842 22.711257413547703 17 22.34594430010757 27.883039709695694 27.206694569023394 22.564321421173346 18 22.215139418440437 26.75816919308785 29.043194487156903 21.983496901314815 19 23.922244319821946 26.37467973411212 27.29451720726155 22.40855873880438 20 24.176867787399697 26.40397483903386 27.68931356139422 21.729843812172223 21 23.72956305574147 25.96941031647385 26.95908826528986 23.341938362494822 22 23.98562542064412 26.943973190284463 26.964532482686387 22.10586890638503 23 23.11586233907178 26.729992381182065 27.447725889979086 22.706419389767067 24 23.677540026934015 26.084941892363712 27.82043992219063 22.417078158511647 25 23.376578015069107 26.47327977645826 27.60091811785839 22.549224090614242 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 898.0 1 898.0 2 1331.0 3 1764.0 4 1764.0 5 1764.0 6 2858.5 7 3953.0 8 3953.0 9 3953.0 10 4720.5 11 5488.0 12 5488.0 13 5488.0 14 7339.5 15 9191.0 16 9191.0 17 9191.0 18 15537.5 19 21884.0 20 21884.0 21 21884.0 22 33706.5 23 45529.0 24 45529.0 25 45529.0 26 65640.0 27 85751.0 28 85751.0 29 85751.0 30 110497.0 31 135243.0 32 135243.0 33 135243.0 34 154335.0 35 173427.0 36 173427.0 37 173427.0 38 189459.0 39 205491.0 40 205491.0 41 205491.0 42 220720.0 43 235949.0 44 235949.0 45 235949.0 46 247448.5 47 258948.0 48 258948.0 49 258948.0 50 255422.5 51 251897.0 52 251897.0 53 251897.0 54 230640.0 55 209383.0 56 209383.0 57 209383.0 58 183122.0 59 156861.0 60 156861.0 61 156861.0 62 130889.0 63 104917.0 64 104917.0 65 104917.0 66 81655.0 67 58393.0 68 58393.0 69 58393.0 70 43276.0 71 28159.0 72 28159.0 73 28159.0 74 20002.5 75 11846.0 76 11846.0 77 11846.0 78 8200.5 79 4555.0 80 4555.0 81 4555.0 82 3046.5 83 1538.0 84 1538.0 85 1538.0 86 1036.5 87 535.0 88 535.0 89 535.0 90 356.5 91 178.0 92 178.0 93 178.0 94 127.0 95 76.0 96 76.0 97 76.0 98 62.0 99 48.0 100 48.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3469850917191792 2 0.29623709305920465 3 0.3145282424293032 4 0.3036430203856848 5 0.28942761625566255 6 0.24772578386024766 7 0.35916262322916326 8 0.3149258761112619 9 0.25851159748337643 10 0.23430564709414273 11 0.24832223438318565 12 0.24613524913241303 13 0.2634323142976149 14 0.2536405848793828 15 0.3466371622474653 16 0.4044928629724509 17 0.4218396323478976 18 0.11630785197290922 19 0.1309208897848901 20 0.19240499785774856 21 0.18758368946399973 22 0.15298955913359596 23 0.24996247332126514 24 0.34802888013432065 25 0.38779224833018705 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2011902.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.5011293429413 #Duplication Level Percentage of deduplicated Percentage of total 1 84.7125723426785 61.417571643898036 2 10.273440023838464 14.896720079305247 3 2.546246951822671 5.538173387794963 4 0.9595680531802655 2.782790701479072 5 0.4530189159053304 1.64221915084257 6 0.2770653502268384 1.2052530475946006 7 0.17144085848543072 0.8700759099001973 8 0.11424708231885933 0.6626433993802625 9 0.08028225652606671 0.52384988379056 >10 0.37447899490887654 4.666918736326911 >50 0.020854429751081736 1.036476165693065 >100 0.013965749082309456 2.0541573990442283 >500 0.0016501412343335495 0.8148283694553803 >1k 0.0011000941562223248 1.5647037617052602 >5k 6.87558847638953E-5 0.3236183637895616 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6492 0.32267973290945584 No Hit GGTATCAACGCAGAGTACTTTTTTT 4010 0.19931388308178033 No Hit TATCAACGCAGAGTACTTTTTTTTT 3759 0.18683812631032723 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2743 0.13633864870157691 No Hit GTCCTACAGTGGACATTTCTAAATT 2738 0.13609012765035275 No Hit CTGTAGGACGTGGAATATGGCAAGA 2584 0.12843567927264846 No Hit CTTTAGGACGTGAAATATGGCGAGG 2399 0.11924040037735435 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.9704210244833E-5 0.0 4 0.0 0.0 0.0 4.9704210244833E-5 0.0 5 0.0 0.0 0.0 4.9704210244833E-5 0.0 6 0.0 0.0 0.0 4.9704210244833E-5 0.0 7 0.0 0.0 0.0 4.9704210244833E-5 0.0 8 0.0 0.0 0.0 4.9704210244833E-5 0.0 9 0.0 0.0 0.0 4.9704210244833E-5 0.0 10 0.0 0.0 0.0 4.9704210244833E-5 0.0 11 0.0 0.0 0.0 1.49112630734499E-4 0.0 12 0.0 0.0 0.0 1.49112630734499E-4 0.0 13 0.0 0.0 0.0 1.49112630734499E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAAACCG 40 0.005260954 14.257685 19 GGTATCA 1280 0.0 13.210469 1 TAGGACC 585 0.0 11.694229 4 GTATCAA 2785 0.0 10.778791 1 ATACCGA 80 3.7694527E-4 10.688189 6 GTCCTAG 205 0.0 10.658158 1 GTAGGAC 1660 0.0 10.3603945 3 ATCGCGC 110 6.180526E-6 10.344665 8 GTCTTAT 175 2.3646862E-10 10.31392 1 TGTAGGA 1695 0.0 10.146211 2 CCCGCAC 75 0.0026439854 10.135252 3 GTCCTAC 1500 0.0 10.006311 1 GTCCTAA 820 0.0 9.96306 1 TCCAACG 105 4.09391E-5 9.95501 18 AGGACGT 1595 0.0 9.948094 5 TATCGCG 115 1.0258269E-5 9.913682 7 TCGCGCG 115 1.0473477E-5 9.896378 9 CTGTAGG 1610 0.0 9.794683 1 GTGGTAT 390 0.0 9.743244 1 CTTACAC 205 4.1836756E-11 9.73355 3 >>END_MODULE