FastQCFastQC Report
Thu 26 May 2016
SRR1512269_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512269_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2011902
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT76430.3798892789012586No Hit
GGTATCAACGCAGAGTACTTTTTTT49790.2474772628090235No Hit
TATCAACGCAGAGTACTTTTTTTTT45960.22844055028525245No Hit
CTGTAGGACGTGGAATATGGCAAGA28420.1412593655158154No Hit
GTCCTACAGTGGACATTTCTAAATT27570.13703450764500458No Hit
GTCCTAAAGTGTGTATTTCTCATTT27150.1349469308147216No Hit
CTTTAGGACGTGAAATATGGCGAGG26060.1295291718980348No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23370.11615873934217472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15000.013.8843931
AGTCGTC1552.5465852E-1111.6505758
GTCTTAG2450.011.2565671
TAGGACC7200.011.2174224
GTATTAC1803.6379788E-1211.0948341
CGAACCG1053.4675868E-610.85705812
TGGCGAG16200.010.84247918
GCGAACC1158.820407E-710.73904511
GAGACCG803.764192E-410.6898095
GGCGAGG8050.010.61522719
TCTATAC2450.010.4708683
AGGACCG1002.3936225E-510.4522575
TAGACTG2100.010.4070095
CTAATCC1106.0367183E-610.3651023
CTAGAGC1856.002665E-1110.2717223
GACTGCG750.002637958910.1380447
CACACCG750.002643408710.1355225
CACCTTT4900.010.07654214
GTCTAGG2300.09.923331
TATGGCG18250.09.88443416
Produced by FastQC (version 0.11.5)