FastQCFastQC Report
Thu 26 May 2016
SRR1512268_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512268_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2289737
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT76060.3321778876788033No Hit
CTGTAGGACGTGGAATATGGCAAGA69270.302523826972268No Hit
GTCCTAAAGTGTGTATTTCTCATTT63390.27684402182434054No Hit
GTATCAACGCAGAGTACTTTTTTTT57630.25168829433249323No Hit
CTTTAGGACGTGAAATATGGCGAGG50870.22216525303997794No Hit
GTCCTACAGTGTGCATTTCTCATTT39300.17163543236624992No Hit
GGTATCAACGCAGAGTACTTTTTTT34680.15145844260716404No Hit
TATCAACGCAGAGTACTTTTTTTTT31580.13791976982509344No Hit
CTGAAGGACCTGGAATATGGCGAGA30170.13176185736615165No Hit
CTGTAGGACCTGGAATATGGCGAGA27640.12071255345046178No Hit
GTCCTTCAGTGTGCATTTCTCATTT25740.11241465722919271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10250.013.3477744
GGTATCA11500.013.3005521
TCCAACG3550.012.57853918
TGTAGGA36950.011.3140482
GTCCTAA19850.010.7208491
GTATCAA26000.010.7062151
AGGACCT22100.010.7047695
GTAGGAC37700.010.5849243
GTGTAGG5050.010.5350911
GTCCTAC33300.010.5274651
CTGTAGG35200.010.4990171
TTAGACT2300.010.3271954
GGCGAGG13750.010.29949319
CCAACGA2500.010.26493119
TCCTACA37350.010.2007842
GACGTGG19250.010.1675487
GGACCTG22150.010.1658776
GAACAGT6550.010.1537636
AGGACGT36800.010.1464695
GGACGTG36150.010.1449356