##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512268_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2289737 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.798047548692274 31.0 30.0 34.0 16.0 34.0 2 29.663167429272445 31.0 30.0 34.0 19.0 34.0 3 30.311460224471194 31.0 31.0 34.0 26.0 34.0 4 33.94520768105682 37.0 35.0 37.0 30.0 37.0 5 34.111267800625136 37.0 35.0 37.0 30.0 37.0 6 34.258754171330594 37.0 35.0 37.0 31.0 37.0 7 34.1940939068548 37.0 35.0 37.0 30.0 37.0 8 34.183911077997166 37.0 35.0 37.0 30.0 37.0 9 35.733454977580394 39.0 37.0 39.0 30.0 39.0 10 35.65802753766044 39.0 37.0 39.0 30.0 39.0 11 35.67974138514598 39.0 37.0 39.0 30.0 39.0 12 35.61232534566197 39.0 37.0 39.0 30.0 39.0 13 35.585670319342356 39.0 37.0 39.0 30.0 39.0 14 36.78946796073086 40.0 38.0 41.0 30.0 41.0 15 36.71986695415238 40.0 38.0 41.0 30.0 41.0 16 36.64804516850625 40.0 37.0 41.0 30.0 41.0 17 36.60167608769042 40.0 37.0 41.0 30.0 41.0 18 36.379154025112925 40.0 37.0 41.0 29.0 41.0 19 36.48320265602556 40.0 37.0 41.0 29.0 41.0 20 36.40525833316228 40.0 37.0 41.0 29.0 41.0 21 36.34846185391597 40.0 37.0 41.0 29.0 41.0 22 36.26607509945465 40.0 37.0 41.0 29.0 41.0 23 36.13758479685658 40.0 37.0 41.0 28.0 41.0 24 36.012173450487985 40.0 37.0 41.0 27.0 41.0 25 35.89628983590692 40.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1401934543834393 1101 2 0.2996070487675091 1101 3 -0.10806452495130614 1101 4 -0.3047583165237526 1101 5 -0.39294159241617166 1101 6 -0.5577531633327979 1101 7 -0.6524633509515922 1101 8 -0.819289509920182 1101 9 -0.7944882610399873 1101 10 -0.8646779014530921 1101 11 -0.869744972370853 1101 12 -1.0444112843778868 1101 13 -1.0444125995449483 1101 14 -1.340378995013367 1101 15 -1.5781783233773794 1101 16 -1.676826808138685 1101 17 -1.616359109909574 1101 18 -1.311035039084878 1101 19 -1.5131901696728534 1101 20 -1.7573035320560138 1101 21 -1.6698848771056376 1101 22 -1.747353150702061 1101 23 -1.8472034055272601 1101 24 -2.033410868794263 1101 25 -2.0032711238501193 1102 1 0.7195685232627511 1102 2 -0.06316257489664423 1102 3 -0.4105124439053185 1102 4 -0.4971787334704274 1102 5 -0.7094239042088901 1102 6 -0.7659275736223137 1102 7 -0.7060475196062654 1102 8 -0.8002042437539458 1102 9 -0.9873312758698631 1102 10 -0.9725190808823498 1102 11 -0.9719867298477283 1102 12 -1.3447412421761058 1102 13 -1.0798745694642662 1102 14 -1.3690870635670294 1102 15 -1.5188559383675084 1102 16 -1.571736243755069 1102 17 -1.5489408680990664 1102 18 -1.3775393093950612 1102 19 -1.4451357523234734 1102 20 -1.6103223310328332 1102 21 -1.5602497282310352 1102 22 -1.7568939193827475 1102 23 -1.9506141433251614 1102 24 -1.9664736428096319 1102 25 -1.7839620879616191 1103 1 1.231814552583483 1103 2 0.3878552667149755 1103 3 -0.08069415096439059 1103 4 -0.05293964647200511 1103 5 -0.24622252201288575 1103 6 -0.3025941619331931 1103 7 -0.4746674000353437 1103 8 -0.34260510410043565 1103 9 -0.6559454755047227 1103 10 -0.7369226366677495 1103 11 -0.7640009044087392 1103 12 -0.6287560749256897 1103 13 -0.7210587604016325 1103 14 -1.0575338952509696 1103 15 -1.107395033985071 1103 16 -0.9584008731713993 1103 17 -0.9512263103782814 1103 18 -0.950392360960997 1103 19 -1.0146678899475674 1103 20 -1.0882046484834476 1103 21 -1.2796943032684496 1103 22 -1.4237527536054202 1103 23 -1.3485484890152009 1103 24 -1.4946443095389839 1103 25 -1.4966547452412584 1104 1 1.2235429407179979 1104 2 0.49124994744665784 1104 3 -0.027658576954738834 1104 4 0.022481181717871834 1104 5 -0.2346357191849222 1104 6 -0.2615288119911767 1104 7 -0.35713756799254526 1104 8 -0.35476703878790516 1104 9 -0.424095327747267 1104 10 -0.38424149351135384 1104 11 -0.5451001076406996 1104 12 -0.4798362006130432 1104 13 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1.4771832541063787 1201 17 1.39951598913148 1201 18 1.6982903079256317 1201 19 1.5339060933410593 1201 20 1.6116851242788641 1201 21 1.6262633633057177 1201 22 1.5732186952275597 1201 23 1.6760121436737236 1201 24 1.6717826321562086 1201 25 1.6049770803684567 1202 1 2.6619556269188074 1202 2 1.85789957852543 1202 3 1.5115784118860347 1202 4 1.274560805689326 1202 5 1.213024177209391 1202 6 1.0839133439449 1202 7 1.0627444285050842 1202 8 0.9103294229388084 1202 9 1.1200131065173409 1202 10 1.193950939024056 1202 11 1.266619460937612 1202 12 1.319376498776606 1202 13 1.3280323953227295 1202 14 1.3678730499470788 1202 15 1.4178807844269414 1202 16 1.4334918741608504 1202 17 1.4154087049952082 1202 18 1.614976602685232 1202 19 1.4837631102332978 1202 20 1.4923840655697234 1202 21 1.5117508043459509 1202 22 1.4322248191925553 1202 23 1.6081525419126308 1202 24 1.625436175177967 1202 25 1.7250942147876813 1203 1 2.5988300359227026 1203 2 1.6876609890745655 1203 3 1.2538651920239516 1203 4 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-0.6604693277005751 2314 16 -0.9517004366340416 2314 17 -0.7649875326828663 2314 18 -2.031269142115761 2314 19 -1.4150014703775184 2314 20 -2.0132787774678818 2314 21 -1.4484015761454785 2314 22 -1.1093490801024188 2314 23 -0.8697143732092556 2314 24 -0.7205256207983481 2314 25 -0.6150696204574686 2315 1 -2.331806163059568 2315 2 -1.0914288210743024 2315 3 -0.3918293070503722 2315 4 -0.31872790893546465 2315 5 -0.1751650455509477 2315 6 -0.19700262863538853 2315 7 -0.18908805969307707 2315 8 -0.2219065527204549 2315 9 -0.2108861795200525 2315 10 -0.23268349862626536 2315 11 -0.2967995510714232 2315 12 -0.29835483786555983 2315 13 -0.3137556376053823 2315 14 -0.3867563453621372 2315 15 -0.970195349440786 2315 16 -0.7882792666081926 2315 17 -0.6861445933517487 2315 18 -0.3612022369858252 2315 19 -0.4191434851952778 2315 20 -0.40488087033602227 2315 21 -0.45394226976338814 2315 22 -0.37686491802998745 2315 23 -0.4389213027617487 2315 24 -0.34100647530934225 2315 25 -0.1432825720959059 2316 1 1.2123781190338576 2316 2 0.38054714585242166 2316 3 -0.046503287754060096 2316 4 -0.24928394744860327 2316 5 -0.37590725688868076 2316 6 -0.46503326092600616 2316 7 -0.39691019569198005 2316 8 -0.4492168233112892 2316 9 -0.5837796936778048 2316 10 -0.6054840620244164 2316 11 -0.6499696400710562 2316 12 -0.5573347601193674 2316 13 -0.5538415267820938 2316 14 -0.6852337488700755 2316 15 -0.6209325358967561 2316 16 -0.5906385345727188 2316 17 -0.608277172277738 2316 18 -0.5365096623090153 2316 19 -0.5994038581370305 2316 20 -0.554937405032355 2316 21 -0.5435791867099269 2316 22 -0.7727362802926265 2316 23 -0.6099743918794616 2316 24 -0.8294944329795868 2316 25 -0.834192159761372 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 75207.0 3 6.0 4 639.0 5 3040.0 6 3563.0 7 3385.0 8 3144.0 9 2995.0 10 2896.0 11 2865.0 12 2771.0 13 2837.0 14 2842.0 15 2974.0 16 2939.0 17 3254.0 18 3690.0 19 4243.0 20 5332.0 21 6570.0 22 7982.0 23 9434.0 24 10973.0 25 12954.0 26 14510.0 27 16058.0 28 18404.0 29 22256.0 30 27920.0 31 36002.0 32 47440.0 33 64197.0 34 90203.0 35 128316.0 36 219116.0 37 369041.0 38 1059739.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.215455340258956 19.19096879087939 11.909512613585816 24.684063255275838 2 14.303303622934168 19.7346168856511 42.89996798127408 23.06211151014065 3 19.400723302746666 24.30555707673537 29.192253519584156 27.10146610093381 4 11.968388249280935 16.376791532585546 38.62600763441039 33.02881258372313 5 12.863005658823736 38.03150020147515 36.04473624275128 13.060757896949843 6 31.92121004365302 37.14140364748534 18.120796848191603 12.816589460670036 7 28.223394975958062 31.10899742705233 22.06401248339857 18.603595113591037 8 24.494429081630862 38.431888995201895 20.341781712527293 16.731900210639946 9 26.454504733886115 15.004643468734635 20.221616715261444 38.31923508211781 10 14.897900400799147 28.994640252561243 34.98202619815132 21.12543314848829 11 34.70642111060618 21.64210142890791 25.37421381169657 18.277263648789337 12 23.99655891759133 23.92616120113915 32.42646656436816 19.65081331690136 13 29.66359008311943 21.9385453969038 25.764788926050773 22.633075593926 14 20.31242406237973 22.687479175304855 27.70213144175412 29.297965320561293 15 22.46722040326387 32.59820731684336 22.991799129733973 21.942773150158796 16 21.491615026125757 27.91987574698269 29.221471707181028 21.36703751971052 17 21.296346244343017 28.92598591231179 28.49447864545575 21.283189197889435 18 21.05442889255496 27.190791237053674 31.294000670736267 20.4607791996551 19 23.642404176201996 26.393652606780776 27.570122680525532 22.393820536491695 20 23.54620455836317 27.442420975156807 28.675391437600588 20.335983028879433 21 22.595149168833988 26.677208654347652 27.22921909949139 23.49842307732697 22 22.98543041722981 28.791787496664874 27.47619485012706 20.746587235978254 23 21.74474617264621 28.247854378853514 28.397769832492248 21.60962961600803 24 23.311803679002757 26.472446921615873 29.247212963019777 20.968536436361596 25 22.460801090111786 27.57122620477213 28.736967126974083 21.231005578141996 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 859.0 1 859.0 2 1352.5 3 1846.0 4 1846.0 5 1846.0 6 2817.5 7 3789.0 8 3789.0 9 3789.0 10 4496.5 11 5204.0 12 5204.0 13 5204.0 14 7458.5 15 9713.0 16 9713.0 17 9713.0 18 17263.0 19 24813.0 20 24813.0 21 24813.0 22 40850.0 23 56887.0 24 56887.0 25 56887.0 26 84450.5 27 112014.0 28 112014.0 29 112014.0 30 150274.0 31 188534.0 32 188534.0 33 188534.0 34 210331.5 35 232129.0 36 232129.0 37 232129.0 38 245668.5 39 259208.0 40 259208.0 41 259208.0 42 271087.0 43 282966.0 44 282966.0 45 282966.0 46 290952.0 47 298938.0 48 298938.0 49 298938.0 50 285174.0 51 271410.0 52 271410.0 53 271410.0 54 238772.0 55 206134.0 56 206134.0 57 206134.0 58 176614.5 59 147095.0 60 147095.0 61 147095.0 62 121503.5 63 95912.0 64 95912.0 65 95912.0 66 74339.0 67 52766.0 68 52766.0 69 52766.0 70 38379.5 71 23993.0 72 23993.0 73 23993.0 74 16890.5 75 9788.0 76 9788.0 77 9788.0 78 6758.0 79 3728.0 80 3728.0 81 3728.0 82 2480.5 83 1233.0 84 1233.0 85 1233.0 86 851.0 87 469.0 88 469.0 89 469.0 90 326.0 91 183.0 92 183.0 93 183.0 94 129.0 95 75.0 96 75.0 97 75.0 98 63.0 99 51.0 100 51.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34379494238858 2 0.2925226783687384 3 0.3123502830237709 4 0.30199974931618784 5 0.2872382286699302 6 0.24404549518132432 7 0.36279275742148553 8 0.3130927263698844 9 0.25697274403130144 10 0.23151130457340735 11 0.24522466990750466 12 0.24334672497321747 13 0.26356738787031003 14 0.2528674690586736 15 0.3452798290808071 16 0.40305939066364393 17 0.4188253934840552 18 0.11783012634202093 19 0.13390184112847894 20 0.19403975216367644 21 0.18661531870254094 22 0.15141476946915738 23 0.2525617570926268 24 0.34602227242692063 25 0.387380734119246 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2289737.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.9077952128427 #Duplication Level Percentage of deduplicated Percentage of total 1 87.00150215300827 62.560862000282114 2 9.04503146070527 13.00816539940228 3 1.9918051187852437 4.2967894375650335 4 0.6979357400119406 2.007480810580098 5 0.3582803517985215 1.2881575082956664 6 0.2113447117147664 0.9118399349581618 7 0.13826587363858384 0.6959675888569651 8 0.09024714468309354 0.519157855873254 9 0.06409034787872345 0.4147736049344785 >10 0.34835619266061457 4.511810421504118 >50 0.027138112050765856 1.3549104343872655 >100 0.021062915031300734 3.104787138185559 >500 0.0023784597935912286 1.1651514743102602 >1k 0.002256487496481682 2.769016311898906 >5k 3.049307427677949E-4 1.391130078965777 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 7606 0.3321778876788033 No Hit CTGTAGGACGTGGAATATGGCAAGA 6927 0.302523826972268 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6339 0.27684402182434054 No Hit GTATCAACGCAGAGTACTTTTTTTT 5763 0.25168829433249323 No Hit CTTTAGGACGTGAAATATGGCGAGG 5087 0.22216525303997794 No Hit GTCCTACAGTGTGCATTTCTCATTT 3930 0.17163543236624992 No Hit GGTATCAACGCAGAGTACTTTTTTT 3468 0.15145844260716404 No Hit TATCAACGCAGAGTACTTTTTTTTT 3158 0.13791976982509344 No Hit CTGAAGGACCTGGAATATGGCGAGA 3017 0.13176185736615165 No Hit CTGTAGGACCTGGAATATGGCGAGA 2764 0.12071255345046178 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2574 0.11241465722919271 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.367313800667937E-5 2 0.0 0.0 0.0 0.0 4.367313800667937E-5 3 0.0 0.0 0.0 0.0 4.367313800667937E-5 4 0.0 0.0 0.0 4.367313800667937E-5 4.367313800667937E-5 5 0.0 0.0 0.0 4.367313800667937E-5 4.367313800667937E-5 6 0.0 0.0 0.0 4.367313800667937E-5 8.734627601335873E-5 7 0.0 0.0 0.0 8.734627601335873E-5 8.734627601335873E-5 8 0.0 0.0 0.0 8.734627601335873E-5 8.734627601335873E-5 9 0.0 0.0 0.0 8.734627601335873E-5 8.734627601335873E-5 10 0.0 0.0 0.0 8.734627601335873E-5 8.734627601335873E-5 11 0.0 0.0 0.0 8.734627601335873E-5 8.734627601335873E-5 12 0.0 0.0 0.0 8.734627601335873E-5 1.310194140200381E-4 13 0.0 4.367313800667937E-5 0.0 8.734627601335873E-5 1.310194140200381E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 1025 0.0 13.347774 4 GGTATCA 1150 0.0 13.300552 1 TCCAACG 355 0.0 12.578539 18 TGTAGGA 3695 0.0 11.314048 2 GTCCTAA 1985 0.0 10.720849 1 GTATCAA 2600 0.0 10.706215 1 AGGACCT 2210 0.0 10.704769 5 GTAGGAC 3770 0.0 10.584924 3 GTGTAGG 505 0.0 10.535091 1 GTCCTAC 3330 0.0 10.527465 1 CTGTAGG 3520 0.0 10.499017 1 TTAGACT 230 0.0 10.327195 4 GGCGAGG 1375 0.0 10.299493 19 CCAACGA 250 0.0 10.264931 19 TCCTACA 3735 0.0 10.200784 2 GACGTGG 1925 0.0 10.167548 7 GGACCTG 2215 0.0 10.165877 6 GAACAGT 655 0.0 10.153763 6 AGGACGT 3680 0.0 10.146469 5 GGACGTG 3615 0.0 10.144935 6 >>END_MODULE