FastQCFastQC Report
Thu 26 May 2016
SRR1512268_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512268_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2289737
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT76810.3354533730293042No Hit
CTGTAGGACGTGGAATATGGCAAGA74370.32479712735567445No Hit
GTATCAACGCAGAGTACTTTTTTTT66880.29208594698867163No Hit
GTCCTAAAGTGTGTATTTCTCATTT64600.28212847152314874No Hit
CTTTAGGACGTGAAATATGGCGAGG55940.24430753400936442No Hit
GTCCTACAGTGTGCATTTCTCATTT40990.17901619268937874No Hit
GGTATCAACGCAGAGTACTTTTTTT40920.17871048072333198No Hit
TATCAACGCAGAGTACTTTTTTTTT40470.1767451895130314No Hit
CTGAAGGACCTGGAATATGGCGAGA33370.14573726152828906No Hit
CTGTAGGACCTGGAATATGGCGAGA29860.1304079900879446No Hit
GTCCTTCAGTGTGCATTTCTCATTT27030.11804849203205434No Hit
ATTTAGAAATGTCCACTGTAGGACG24250.10590735966619748No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAACC400.00526755414.25511559
GTTCCGG500.001488252813.3137961
TAGGACC10150.013.1064324
GGTATCA12000.012.0458151
TCCAACG3450.011.55875718
AAGATCG752.0702479E-411.4025965
CCAACGT1751.8189894E-1211.39388219
GATATAC9100.011.286421
GACGTGG18200.011.2275387
CCAACGA2050.011.11598219
TGTAGGA37700.011.1147782
AGGACCT21600.011.0418665
AATGTCC8650.010.9875628
GTAGGAC37350.010.8884533
AGTCGTC2800.010.861998
GTATCAA24950.010.8248431
AAATGTC8800.010.8002747
ACTGTTC9600.010.7912748
GCCGGTT2400.010.68713111
GTCCTAC37350.010.668351
Produced by FastQC (version 0.11.5)