FastQCFastQC Report
Thu 26 May 2016
SRR1512267_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512267_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1709510
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60970.35665190610174846No Hit
GTCCTACAGTGGACATTTCTAAATT54840.3207936777205164No Hit
GTCCTAAAGTGTGTATTTCTCATTT53680.3140081075863844No Hit
CTGTAGGACGTGGAATATGGCAAGA48450.28341454568853064No Hit
CTTTAGGACGTGAAATATGGCGAGG44790.26200490198945897No Hit
GGTATCAACGCAGAGTACTTTTTTT39700.23223028821124184No Hit
TATCAACGCAGAGTACTTTTTTTTT36450.21321899257682025No Hit
GTCCTACAGTGTGCATTTCTCATTT33740.19736649683242566No Hit
CTGTAGGACCTGGAATATGGCGAGA24170.14138554322583663No Hit
CTGAAGGACCTGGAATATGGCGAGA23200.13571140268264004No Hit
GTCCTTCAGTGTGCATTTCTCATTT21330.1247725956560652No Hit
ATTTAGAAATGTCCACTGTAGGACG20130.11775304034489416No Hit
TTTCTAAATTTTCCACCTTTTTCAG18580.10868611473463156No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17800.10412340378237038No Hit
GAATATGGCAAGAAAACTGAAAATC17520.10248550754309714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1400.014.93118218
GTGTAGG2700.014.4263271
GCGATCA400.0053363214.2269739
CGATACT500.001499001813.3007624
GGTATCA11250.013.2581471
TAGGACC10200.013.1331044
CCAACGA1207.3305273E-1012.67409919
CCAACGT550.003055196712.09800519
ACGGGAA550.003112874712.067838
GATTTAC802.8659173E-511.875331
GCCGGTT1052.743218E-711.75428511
GGACCTG18500.011.4520076
CTATACT2500.011.4006534
AGGACCT19550.011.1771115
CTTAGAC1451.2169039E-911.1385693
GGCGTGC951.38940595E-510.9790038
CTCGAAC1408.3273335E-910.85904118
AAGGCGT1408.341885E-910.8577656
CGTTTCC1502.0972948E-910.777740514
CCGTTTC1502.1300366E-910.767613
Produced by FastQC (version 0.11.5)