##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512267_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1709510 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.127400834157157 31.0 30.0 34.0 16.0 34.0 2 30.011333657012827 31.0 30.0 34.0 23.0 34.0 3 30.668712087089283 33.0 31.0 34.0 26.0 34.0 4 34.322325110704234 37.0 35.0 37.0 32.0 37.0 5 34.500971038484714 37.0 35.0 37.0 32.0 37.0 6 34.65454077484191 37.0 35.0 37.0 32.0 37.0 7 34.598121683991316 37.0 35.0 37.0 32.0 37.0 8 34.604715386280276 37.0 35.0 37.0 32.0 37.0 9 36.20303303285737 39.0 37.0 39.0 32.0 39.0 10 36.134663149089505 39.0 37.0 39.0 32.0 39.0 11 36.156113155231616 39.0 37.0 39.0 32.0 39.0 12 36.10439248673597 39.0 37.0 39.0 32.0 39.0 13 36.080740095114976 39.0 37.0 39.0 32.0 39.0 14 37.32912881468959 40.0 38.0 41.0 32.0 41.0 15 37.26282502003498 40.0 38.0 41.0 32.0 41.0 16 37.19420945183123 40.0 38.0 41.0 32.0 41.0 17 37.16560066919761 40.0 38.0 41.0 31.0 41.0 18 36.950753724751536 40.0 38.0 41.0 31.0 41.0 19 37.068763563828234 40.0 38.0 41.0 31.0 41.0 20 37.0031254570023 40.0 38.0 41.0 31.0 41.0 21 36.95370252294517 40.0 38.0 41.0 31.0 41.0 22 36.87662371088791 40.0 38.0 41.0 31.0 41.0 23 36.763749261484286 40.0 38.0 41.0 31.0 41.0 24 36.650624447941226 40.0 38.0 41.0 30.0 41.0 25 36.54108955197688 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8460612686711713 1101 2 0.07147764205371843 1101 3 -0.3793203466092443 1101 4 -0.5699787858151737 1101 5 -0.740858177923748 1101 6 -0.7844772525821568 1101 7 -0.7592318342121942 1101 8 -0.7461684553836889 1101 9 -1.0179918381015867 1101 10 -0.9263042433357498 1101 11 -1.0635531435196413 1101 12 -1.2241465511223808 1101 13 -1.1548139126329175 1101 14 -1.423323524025541 1101 15 -1.7141698859826136 1101 16 -1.6710261605015901 1101 17 -1.527141575552612 1101 18 -1.345714602006261 1101 19 -1.5631370865179832 1101 20 -1.5188562754249375 1101 21 -1.5981553420558754 1101 22 -1.7613701151519408 1101 23 -1.7621416034407673 1101 24 -1.8357328323929991 1101 25 -1.7591976764926684 1102 1 1.1344237192362066 1102 2 0.4084728387060821 1102 3 -0.056744382952942374 1102 4 -0.09318641419290685 1102 5 -0.22034554003428042 1102 6 -0.34564679727503744 1102 7 -0.3455787474180312 1102 8 -0.45603882932798 1102 9 -0.6836284501485537 1102 10 -0.6736149966464708 1102 11 -0.7045442628405567 1102 12 -0.7897863031127983 1102 13 -0.6309047749379673 1102 14 -1.0096191259603557 1102 15 -0.9990745695528958 1102 16 -1.1401072905136331 1102 17 -1.092277787831172 1102 18 -0.9460855748373262 1102 19 -0.9833354237264658 1102 20 -1.0204228008707332 1102 21 -1.188086385834346 1102 22 -1.1677044532671772 1102 23 -1.271152858223438 1102 24 -1.4348842052375446 1102 25 -1.5585273368580062 1103 1 1.1140719805050239 1103 2 0.2615340835657456 1103 3 -0.12021719691982469 1103 4 -0.297150818256128 1103 5 -0.5450115964196982 1103 6 -0.4045938339888693 1103 7 -0.5387954999233173 1103 8 -0.3555926681110648 1103 9 -0.7337527878222971 1103 10 -0.7494174879628019 1103 11 -0.7621416939557761 1103 12 -0.8637893202976343 1103 13 -0.9553127450969257 1103 14 -1.1956394078304413 1103 15 -1.2706525743324875 1103 16 -1.2635373847935227 1103 17 -1.2804725531010348 1103 18 -1.2200818903356208 1103 19 -1.4768255194892888 1103 20 -1.3131004240819522 1103 21 -1.2841828673063205 1103 22 -1.2790429939535457 1103 23 -1.2820103033694252 1103 24 -1.2358776233258197 1103 25 -1.446408833929226 1104 1 1.2883997416961819 1104 2 0.48099667713590577 1104 3 -0.11392183698334435 1104 4 0.1223996191429535 1104 5 -0.18825850763424512 1104 6 -0.12475560674464958 1104 7 -0.18389475520820042 1104 8 -0.3026477020294962 1104 9 -0.5147170506999359 1104 10 -0.4150312649521126 1104 11 -0.3338408710809375 1104 12 -0.40184273126870096 1104 13 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24 -0.5434733746581912 1105 25 -0.5041583529601397 1106 1 0.9557951373522862 1106 2 0.21448410902720383 1106 3 -0.2997168175006948 1106 4 -0.46921322319278147 1106 5 -0.5083944800451476 1106 6 -0.6035052314459293 1106 7 -0.5341470315819592 1106 8 -0.5522929563628196 1106 9 -0.8968012353406607 1106 10 -0.7774391091803707 1106 11 -0.8629063386332589 1106 12 -0.8252033623427479 1106 13 -0.7490926072513204 1106 14 -0.8535448406986177 1106 15 -0.9333669861861154 1106 16 -1.028793070574416 1106 17 -1.0567777782731085 1106 18 -0.8241451789150673 1106 19 -0.85992137938252 1106 20 -0.8266757536293241 1106 21 -0.9831383134224652 1106 22 -1.0800641887000069 1106 23 -1.2477134167419948 1106 24 -1.260369345181232 1106 25 -1.1385614318323718 1107 1 0.10987848960484214 1107 2 -0.29557647311599666 1107 3 -0.5782377355578596 1107 4 -0.45425357827483737 1107 5 -0.45159643525153825 1107 6 -0.3060440133121247 1107 7 -0.2045804011284602 1107 8 -0.24474639602980375 1107 9 -0.30409539547193987 1107 10 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15 1.1274631957728687 1201 16 1.2502674803348341 1201 17 1.0854630951084232 1201 18 1.3741789150338874 1201 19 1.3497658756636142 1201 20 1.1827250782525525 1201 21 1.2120871352014788 1201 22 1.1681178404387964 1201 23 1.1997074256362197 1201 24 1.2862931436377423 1201 25 1.1916701081744492 1202 1 2.51224719488577 1202 2 1.7330746143948126 1202 3 1.303697580749894 1202 4 0.9794568812764695 1202 5 0.9487855475750706 1202 6 0.8798558793145972 1202 7 0.9422525621524116 1202 8 0.8806728823837346 1202 9 0.9838542499428371 1202 10 1.0326619324565414 1202 11 1.1573175990213045 1202 12 1.0716675420366997 1202 13 1.0770368623514912 1202 14 1.145068170031287 1202 15 1.2510886371320495 1202 16 1.193275004825189 1202 17 1.143066251860695 1202 18 1.3914911323046013 1202 19 1.3193313735055483 1202 20 1.187094499037876 1202 21 1.220779001291909 1202 22 1.2997031717491438 1202 23 1.2937544403795087 1202 24 1.327862236856724 1202 25 1.4490291326549851 1203 1 2.6589462352526425 1203 2 1.8380107830219536 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-0.17126438944549705 2314 8 -0.18427130399973635 2314 9 -0.0704312608644102 2314 10 -0.09908975926516206 2314 11 -0.9978824422357064 2314 12 -0.4324630707895665 2314 13 -0.30869656086319 2314 14 -0.22133523760150808 2314 15 -0.3225870557340542 2314 16 -0.6527203021896071 2314 17 -0.5483754965525662 2314 18 -1.8033236224801712 2314 19 -1.0867401607518943 2314 20 -1.7067141249056803 2314 21 -0.8720928674420279 2314 22 -0.6381264392438837 2314 23 -0.3759659819645975 2314 24 -0.2866382524563562 2314 25 -0.17787486006609754 2315 1 -2.6378014667393117 2315 2 -1.1952118475806337 2315 3 -0.504689760979673 2315 4 -0.40755394923929344 2315 5 -0.29442381275885765 2315 6 -0.2909336885942011 2315 7 -0.2561474916757547 2315 8 -0.2484301336758037 2315 9 -0.28995161780807877 2315 10 -0.30164247063954264 2315 11 -0.47831208240725687 2315 12 -0.46083265621436453 2315 13 -0.38770881156995785 2315 14 -0.571349661510574 2315 15 -0.9903018597932629 2315 16 -0.8649428022056398 2315 17 -0.7233586686586477 2315 18 -0.4952423870242768 2315 19 -0.6979741421995413 2315 20 -0.6484710211368778 2315 21 -0.5141792698626801 2315 22 -0.4781479789481011 2315 23 -0.6402594347920072 2315 24 -0.36627553922290446 2315 25 -0.31938430612387947 2316 1 1.512937655798627 2316 2 0.7511899121868844 2316 3 0.25761885757591685 2316 4 0.049492520462571576 2316 5 -0.06080407995291637 2316 6 -0.17656864446547615 2316 7 -0.06465253015385031 2316 8 -0.21756390560562977 2316 9 -0.2367864243412825 2316 10 -0.20741553059002626 2316 11 -0.397999481503156 2316 12 -0.2922990955104581 2316 13 -0.21561468149784702 2316 14 -0.3540166914320082 2316 15 -0.3285979931481222 2316 16 -0.3727541249911255 2316 17 -0.1906839027561631 2316 18 -0.10627658678749441 2316 19 -0.28194653483758003 2316 20 -0.22023250402354222 2316 21 -0.21579861250609866 2316 22 -0.3267830828120495 2316 23 -0.292172912889086 2316 24 -0.3952059221203328 2316 25 -0.301767627348724 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44454.0 3 3.0 4 358.0 5 1778.0 6 2133.0 7 1960.0 8 1848.0 9 1744.0 10 1701.0 11 1716.0 12 1758.0 13 1741.0 14 1796.0 15 1794.0 16 1916.0 17 2107.0 18 2301.0 19 2814.0 20 3358.0 21 4139.0 22 5025.0 23 6252.0 24 7315.0 25 8625.0 26 9468.0 27 10663.0 28 12321.0 29 15063.0 30 19069.0 31 24815.0 32 32465.0 33 44659.0 34 62756.0 35 91480.0 36 159327.0 37 271613.0 38 847175.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.41644092121753 19.93367045958154 12.164661616621126 24.48522700257981 2 13.882249405966734 20.453870750095337 43.49755052949632 22.166329314441608 3 18.615150709641227 25.60142145359826 30.078202246136097 25.705225590624416 4 11.660185037411166 17.289521118298143 39.70840882847192 31.341885015818775 5 12.121965885924645 38.7160435885778 36.65506064561548 12.506929879882081 6 30.329409860598656 38.245180297567806 18.85512862313316 12.570281218700375 7 26.787305769752397 31.85059022041236 23.33076179706939 18.031342212765857 8 23.674801556913426 38.85183209605309 21.178705283189593 16.294661063843897 9 26.475504346428725 14.879788490614507 20.804268191836385 37.840438971120385 10 14.632528887744863 29.092144679418887 35.81054259766013 20.464783835176117 11 33.6203474076372 21.86019836282934 26.470113924043208 18.04934030549025 12 23.562868562903745 24.436805389404153 33.218454299372766 18.78187174831933 13 29.32199811144803 22.60115776447059 26.1562688781884 21.920575245892987 14 19.932064508221288 23.388599340702005 28.455493866511215 28.223842284565492 15 21.983722370440812 33.23277949476539 23.60226935866844 21.18122877612536 16 20.64146253504979 28.283344434146336 30.162083616727102 20.913109414076768 17 20.596660001080906 29.419201116622684 29.086705093651087 20.89743378864532 18 20.322903507862634 27.373240636788022 32.32525547479454 19.97860038055481 19 23.16808880516937 26.697487477215326 28.319767959477254 21.814655758138052 20 23.23130023191739 27.734905002956843 29.353813801458315 19.679980963667447 21 22.032013915832188 26.84652614082982 27.989788179747727 23.131671763590266 22 22.605392491427587 29.357460093536403 28.203399204067658 19.833748210968352 23 21.105121546715083 28.748736209731714 29.247156325284955 20.898985918268252 24 22.874665990429364 26.890235884720326 29.993883339673054 20.241214785177252 25 21.831931697331186 28.031330249557957 29.55826297748946 20.578475075621398 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 912.0 1 912.0 2 1342.5 3 1773.0 4 1773.0 5 1773.0 6 2699.5 7 3626.0 8 3626.0 9 3626.0 10 4348.5 11 5071.0 12 5071.0 13 5071.0 14 7025.5 15 8980.0 16 8980.0 17 8980.0 18 15908.0 19 22836.0 20 22836.0 21 22836.0 22 36892.5 23 50949.0 24 50949.0 25 50949.0 26 74900.5 27 98852.0 28 98852.0 29 98852.0 30 128566.5 31 158281.0 32 158281.0 33 158281.0 34 173131.5 35 187982.0 36 187982.0 37 187982.0 38 195042.5 39 202103.0 40 202103.0 41 202103.0 42 207642.5 43 213182.0 44 213182.0 45 213182.0 46 215416.5 47 217651.0 48 217651.0 49 217651.0 50 204337.5 51 191024.0 52 191024.0 53 191024.0 54 164068.5 55 137113.0 56 137113.0 57 137113.0 58 116071.5 59 95030.0 60 95030.0 61 95030.0 62 77392.5 63 59755.0 64 59755.0 65 59755.0 66 45461.0 67 31167.0 68 31167.0 69 31167.0 70 22806.5 71 14446.0 72 14446.0 73 14446.0 74 10021.5 75 5597.0 76 5597.0 77 5597.0 78 3834.5 79 2072.0 80 2072.0 81 2072.0 82 1389.5 83 707.0 84 707.0 85 707.0 86 467.5 87 228.0 88 228.0 89 228.0 90 159.0 91 90.0 92 90.0 93 90.0 94 66.0 95 42.0 96 42.0 97 42.0 98 41.5 99 41.0 100 41.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3448356546612772 2 0.2959912489543787 3 0.3123117150528514 4 0.3045317079163035 5 0.28867921217190895 6 0.24673736918766195 7 0.36548484653497204 8 0.31406660388064417 9 0.2592555761592503 10 0.23568156957256758 11 0.248667746898234 12 0.24790729507285716 13 0.2632333241689139 14 0.25521933185532697 15 0.34776046937426514 16 0.4054963118086469 17 0.42316219267509403 18 0.11716807740229655 19 0.1299787658451837 20 0.19297927476294377 21 0.19022994893273512 22 0.15097893548443705 23 0.25282098379067686 24 0.3489888915537201 25 0.38718697170534244 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1709510.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.61696667060889 #Duplication Level Percentage of deduplicated Percentage of total 1 84.86198635344584 59.07834075569506 2 10.549047227606339 14.687853385018993 3 2.4549835414815404 5.127255221526413 4 0.8357922792705651 2.327412929981247 5 0.3860437742175996 1.3437598281551346 6 0.21497255516159888 0.8979442324668395 7 0.12922376179221076 0.6297316422417327 8 0.08267858676706422 0.46046659354686037 9 0.07172987256457286 0.4494254532853402 >10 0.3545041068473177 4.485520895451374 >50 0.027428857351117407 1.3266317820689095 >100 0.026369594615186107 3.7500671910344066 >500 0.0026197444394871726 1.2900642856866784 >1k 0.0023662207840529303 3.1483851809796883 >5k 2.535236554342425E-4 0.9971406228613422 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6097 0.35665190610174846 No Hit GTCCTACAGTGGACATTTCTAAATT 5484 0.3207936777205164 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5368 0.3140081075863844 No Hit CTGTAGGACGTGGAATATGGCAAGA 4845 0.28341454568853064 No Hit CTTTAGGACGTGAAATATGGCGAGG 4479 0.26200490198945897 No Hit GGTATCAACGCAGAGTACTTTTTTT 3970 0.23223028821124184 No Hit TATCAACGCAGAGTACTTTTTTTTT 3645 0.21321899257682025 No Hit GTCCTACAGTGTGCATTTCTCATTT 3374 0.19736649683242566 No Hit CTGTAGGACCTGGAATATGGCGAGA 2417 0.14138554322583663 No Hit CTGAAGGACCTGGAATATGGCGAGA 2320 0.13571140268264004 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2133 0.1247725956560652 No Hit ATTTAGAAATGTCCACTGTAGGACG 2013 0.11775304034489416 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1858 0.10868611473463156 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1780 0.10412340378237038 No Hit GAATATGGCAAGAAAACTGAAAATC 1752 0.10248550754309714 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 5.8496294259758647E-5 0.0 2 0.0 0.0 0.0 5.8496294259758647E-5 0.0 3 0.0 0.0 0.0 1.1699258851951729E-4 0.0 4 0.0 0.0 0.0 1.1699258851951729E-4 0.0 5 0.0 0.0 0.0 1.1699258851951729E-4 0.0 6 0.0 0.0 0.0 1.1699258851951729E-4 0.0 7 0.0 0.0 0.0 1.1699258851951729E-4 0.0 8 0.0 0.0 0.0 1.1699258851951729E-4 0.0 9 0.0 0.0 0.0 1.7548888277927593E-4 0.0 10 0.0 0.0 0.0 1.7548888277927593E-4 0.0 11 0.0 0.0 0.0 2.3398517703903459E-4 0.0 12 0.0 0.0 0.0 2.3398517703903459E-4 0.0 13 0.0 0.0 0.0 2.924814712987932E-4 5.8496294259758647E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 140 0.0 14.931182 18 GTGTAGG 270 0.0 14.426327 1 GCGATCA 40 0.00533632 14.226973 9 CGATACT 50 0.0014990018 13.300762 4 GGTATCA 1125 0.0 13.258147 1 TAGGACC 1020 0.0 13.133104 4 CCAACGA 120 7.3305273E-10 12.674099 19 CCAACGT 55 0.0030551967 12.098005 19 ACGGGAA 55 0.0031128747 12.06783 8 GATTTAC 80 2.8659173E-5 11.87533 1 GCCGGTT 105 2.743218E-7 11.754285 11 GGACCTG 1850 0.0 11.452007 6 CTATACT 250 0.0 11.400653 4 AGGACCT 1955 0.0 11.177111 5 CTTAGAC 145 1.2169039E-9 11.138569 3 GGCGTGC 95 1.38940595E-5 10.979003 8 CTCGAAC 140 8.3273335E-9 10.859041 18 AAGGCGT 140 8.341885E-9 10.857765 6 CGTTTCC 150 2.0972948E-9 10.7777405 14 CCGTTTC 150 2.1300366E-9 10.7676 13 >>END_MODULE