FastQCFastQC Report
Thu 26 May 2016
SRR1512267_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512267_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1709510
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69330.4055548081029067No Hit
GTCCTACAGTGGACATTTCTAAATT55630.3254148849670373No Hit
GTCCTAAAGTGTGTATTTCTCATTT54460.3185708185386456No Hit
CTGTAGGACGTGGAATATGGCAAGA52630.30786599668910974No Hit
CTTTAGGACGTGAAATATGGCGAGG46940.27458160525530706No Hit
GGTATCAACGCAGAGTACTTTTTTT46240.27048686465712396No Hit
TATCAACGCAGAGTACTTTTTTTTT44430.25989903539610765No Hit
GTCCTACAGTGTGCATTTCTCATTT33110.19368123029406087No Hit
CTGAAGGACCTGGAATATGGCGAGA26650.1558926242022568No Hit
CTGTAGGACCTGGAATATGGCGAGA26290.15378675760890548No Hit
ATTTAGAAATGTCCACTGTAGGACG21950.1283993659001702No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21790.1274634251920141No Hit
TTTCTAAATTTTCCACCTTTTTCAG21360.12494808453884447No Hit
GTCCTTCAGTGTGCATTTCTCATTT20180.11804552181619295No Hit
GAATATGGCAAGAAAACTGAAAATC18820.11009002579686576No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18370.10745769255517663No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACGA400.00526795814.2546555
GGTATCA11250.014.1163381
TAGGACC10100.013.83044054
ACCGTGC500.001493987313.3066838
TCCAACG2150.013.24734418
CCAACGA1207.4760464E-1012.65857319
ACCTTTT6800.012.42587915
CCCGTAC853.9722418E-612.286262516
ATCCCGT1459.822543E-1111.79281310
CGCGCGC658.025798E-411.69201910
CCAACGT1157.137169E-811.55782819
AGGACCT19650.011.3650365
GGCGAGG12900.011.26024219
ACGTGTC951.35274895E-511.00552758
CCGTACA951.36997405E-510.9929717
TGTCCCG1053.4672903E-610.85687511
TGTAGGA29900.010.8055722
GCCGGTT1502.1336746E-910.76640111
GGACCTG19800.010.7528756
CAATCTA803.7556532E-410.6922459
Produced by FastQC (version 0.11.5)