FastQCFastQC Report
Thu 26 May 2016
SRR1512266_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512266_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2480393
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT96090.3873982872875387No Hit
GTCCTAAAGTGTGTATTTCTCATTT87460.3526054137388712No Hit
CTGTAGGACGTGGAATATGGCAAGA86500.3487350593232605No Hit
CTTTAGGACGTGAAATATGGCGAGG77720.3133374428971538No Hit
GTATCAACGCAGAGTACTTTTTTTT66740.2690702642686058No Hit
GTCCTACAGTGTGCATTTCTCATTT53090.21403866242164044No Hit
CTGAAGGACCTGGAATATGGCGAGA41020.1653770188837011No Hit
CTGTAGGACCTGGAATATGGCGAGA40920.1649738569654083No Hit
GGTATCAACGCAGAGTACTTTTTTT38750.15622524333845483No Hit
TATCAACGCAGAGTACTTTTTTTTT38450.15501575758357647No Hit
GTCCTTCAGTGTGCATTTCTCATTT34000.13707505221954747No Hit
ATTTAGAAATGTCCACTGTAGGACG32850.13243869015918042No Hit
TTTCTAAATTTTCCACCTTTTTCAG30430.12268217173649498No Hit
GAATATGGCAAGAAAACTGAAAATC28960.11675569153759102No Hit
GGAATATGGCGAGAAAACTGAAAAT25260.10183870056075792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1450.013.76898819
TAGGACC13050.012.9578954
GGTATCA13450.012.855061
TCCAACG2900.012.78056318
AAATGTC11200.012.2991467
GGCGAGG23250.011.776607519
TGGCGAA1950.011.69661118
GCCGGTT2150.011.48003511
ATAGGAC4100.011.3543853
TAGAAAT12900.011.3411234
AATGTCC12350.011.2882878
CACCTTT11300.011.19233714
TTTAGAA13450.011.089932
ATTTAGA11850.010.9831571
TGTCCAC13500.010.9675910
CCACCTT11700.010.80071213
ATGTCCA13200.010.7786729
GAAATGT13300.010.714326
AGGACCT28400.010.7042595
GACGTGA28300.010.5742397
Produced by FastQC (version 0.11.5)