##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512266_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2480393 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.144818180022277 31.0 30.0 34.0 16.0 34.0 2 30.034208288767143 31.0 30.0 34.0 24.0 34.0 3 30.688977512837685 33.0 31.0 34.0 27.0 34.0 4 34.336161245415546 37.0 35.0 37.0 32.0 37.0 5 34.512925975843345 37.0 35.0 37.0 32.0 37.0 6 34.66668749669911 37.0 35.0 37.0 32.0 37.0 7 34.608744259478236 37.0 35.0 37.0 32.0 37.0 8 34.60718442601636 37.0 35.0 37.0 32.0 37.0 9 36.210871019229614 39.0 37.0 39.0 32.0 39.0 10 36.13866834812064 39.0 37.0 39.0 32.0 39.0 11 36.1602572656833 39.0 37.0 39.0 32.0 39.0 12 36.109269377876814 39.0 37.0 39.0 32.0 39.0 13 36.08861579596459 39.0 37.0 39.0 32.0 39.0 14 37.336126170328654 40.0 38.0 41.0 32.0 41.0 15 37.2749612662187 40.0 38.0 41.0 32.0 41.0 16 37.2080496921254 40.0 38.0 41.0 32.0 41.0 17 37.17462595645126 40.0 38.0 41.0 32.0 41.0 18 36.96716770285999 40.0 38.0 41.0 31.0 41.0 19 37.077784851029655 40.0 38.0 41.0 31.0 41.0 20 37.020891044282095 40.0 38.0 41.0 31.0 41.0 21 36.960730013348694 40.0 38.0 41.0 31.0 41.0 22 36.889827136264294 40.0 38.0 41.0 31.0 41.0 23 36.77485503305323 40.0 38.0 41.0 31.0 41.0 24 36.66128472383207 40.0 38.0 41.0 30.0 41.0 25 36.552936974100476 40.0 38.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2024688194647553 1101 2 0.4049322053979907 1101 3 -0.14473598662955922 1101 4 -0.16065661798091924 1101 5 -0.43792663307419843 1101 6 -0.5119034068249775 1101 7 -0.45075443090316725 1101 8 -0.4212694197357507 1101 9 -0.46154790272547785 1101 10 -0.5925233898998101 1101 11 -0.5016380301176184 1101 12 -0.6765638095943913 1101 13 -0.7673514636424201 1101 14 -0.9349032769359908 1101 15 -1.0602798369899133 1101 16 -1.0841804886641313 1101 17 -1.2011616418175564 1101 18 -1.201184131846432 1101 19 -1.20799946555573 1101 20 -1.0783353964634301 1101 21 -1.3911824612980013 1101 22 -1.4581864276449679 1101 23 -1.5314944598927767 1101 24 -1.6936747753287733 1101 25 -1.6948905794481064 1102 1 1.0201998285559242 1102 2 0.1988452735786126 1102 3 -0.26562870436417896 1102 4 -0.2944436330594513 1102 5 -0.5530978263876491 1102 6 -0.5498406839909933 1102 7 -0.5570468101300392 1102 8 -0.7096017745810883 1102 9 -0.735939083287029 1102 10 -0.8745783409438488 1102 11 -0.7696299342692825 1102 12 -0.9532011286354845 1102 13 -0.8973881341458778 1102 14 -1.1227147535129518 1102 15 -1.1741649674093892 1102 16 -1.2584293851471102 1102 17 -1.3861276121795427 1102 18 -1.3126449618146765 1102 19 -1.3291329474508942 1102 20 -1.4285746525600445 1102 21 -1.2837953328691754 1102 22 -1.447779208297419 1102 23 -1.3413042754951334 1102 24 -1.499380377400506 1102 25 -1.5650304927098801 1103 1 1.024327585010738 1103 2 0.18152078507790392 1103 3 -0.32690989549517013 1103 4 -0.38170123125421895 1103 5 -0.5754013109293439 1103 6 -0.5874181266456517 1103 7 -0.6982392507195598 1103 8 -0.6645727115490629 1103 9 -0.8157158469647854 1103 10 -0.7584470989399605 1103 11 -0.8839010414851742 1103 12 -0.8236842700621452 1103 13 -0.9127653491317247 1103 14 -1.0347801931857532 1103 15 -1.095656500345811 1103 16 -1.1352187512002843 1103 17 -1.1355110930946495 1103 18 -1.2194725077262092 1103 19 -1.369589997628701 1103 20 -1.38461895731389 1103 21 -1.2041847875721459 1103 22 -1.4748451012930772 1103 23 -1.6402812769155446 1103 24 -1.5511871208298444 1103 25 -1.6799760435978897 1104 1 1.406870771698383 1104 2 0.6423923654118511 1104 3 0.08768393213211212 1104 4 0.12511716308323173 1104 5 -0.08378170342101754 1104 6 -0.10748463963317079 1104 7 -0.1585058718516663 1104 8 -0.1963440467281714 1104 9 -0.2362828335930729 1104 10 -0.3250165981004187 1104 11 -0.22392795566472046 1104 12 -0.19672536907135907 1104 13 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2314 15 -0.7101169162469958 2314 16 -0.8724342248325314 2314 17 -0.8830984876440624 2314 18 -1.8679859003679553 2314 19 -1.3823565236327298 2314 20 -2.0671927495265976 2314 21 -1.500345328548022 2314 22 -1.116187297485908 2314 23 -0.8782111979737408 2314 24 -0.8574738870366616 2314 25 -0.7261660903246039 2315 1 -2.890924620837037 2315 2 -1.3807011542330407 2315 3 -0.7400394638910761 2315 4 -0.5964207992716339 2315 5 -0.4832150528477328 2315 6 -0.34531140868793386 2315 7 -0.40747163977915335 2315 8 -0.4684181639987486 2315 9 -0.49241168025510973 2315 10 -0.5406155450803425 2315 11 -0.7193067764026608 2315 12 -0.6553057989988034 2315 13 -0.6374622421924059 2315 14 -0.6902040038401154 2315 15 -1.3003530952027091 2315 16 -1.1482686074316533 2315 17 -1.0397747190002988 2315 18 -0.7695055968765203 2315 19 -0.9144062410180354 2315 20 -0.913338389992667 2315 21 -0.9949876094974641 2315 22 -0.7584604092400937 2315 23 -0.8322249576906913 2315 24 -0.7105183185653274 2315 25 -0.5355332430973618 2316 1 1.159913311911648 2316 2 0.3526812005826443 2316 3 -0.18069951717206223 2316 4 -0.29301664768850344 2316 5 -0.47840430869844397 2316 6 -0.5876406354762906 2316 7 -0.6281838268489537 2316 8 -0.583303539769922 2316 9 -0.7176858925122858 2316 10 -0.6897639096854533 2316 11 -0.7355660814554312 2316 12 -0.7610959636854915 2316 13 -0.7260328012603736 2316 14 -0.8523549050281076 2316 15 -0.6666437888752768 2316 16 -0.7031263548440805 2316 17 -0.7255528062383547 2316 18 -0.6209969546494278 2316 19 -0.7943651649460435 2316 20 -0.8349100197790307 2316 21 -0.7660492140454664 2316 22 -0.8609705428249583 2316 23 -0.776268449252413 2316 24 -0.8562792615623209 2316 25 -0.9083508576897259 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 64187.0 3 5.0 4 495.0 5 2511.0 6 3072.0 7 2912.0 8 2807.0 9 2605.0 10 2508.0 11 2517.0 12 2500.0 13 2547.0 14 2556.0 15 2651.0 16 2659.0 17 2866.0 18 3225.0 19 4117.0 20 4868.0 21 5802.0 22 7271.0 23 8865.0 24 10248.0 25 12353.0 26 13721.0 27 15367.0 28 17889.0 29 22090.0 30 27602.0 31 35815.0 32 47577.0 33 64797.0 34 91174.0 35 132011.0 36 230328.0 37 394614.0 38 1231261.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.73710459978858 20.026207293297965 12.51444779194047 24.72224031497299 2 13.703000435080071 20.062043388319477 43.74228703315828 22.49266914344218 3 18.5310186984567 25.129653229430097 29.92954257475156 26.40978549736164 4 11.815272354343726 17.002937551156418 39.37276974192901 31.809020352570844 5 12.381463386626374 38.529610246884474 36.4923553917 12.596570974789149 6 30.93552628228627 37.86025810827032 18.576504314670444 12.627711294772967 7 27.458575651375984 31.498081820219497 23.021070442229245 18.022272086175267 8 23.850728352650645 39.07151517896793 20.878916882299603 16.198839586081828 9 26.37763654494531 15.080182171759981 20.995873365807995 37.54630791748672 10 15.001369943836343 28.970351829056874 35.385608881438834 20.642669345667947 11 33.47335278759023 21.72950229975184 26.71996378598162 18.07718112667631 12 24.049737618944462 24.16690377421326 32.91467684935803 18.868681757484246 13 29.205378538235415 22.643291864605196 26.027070552891225 22.124259044268165 14 19.778900614407473 23.62536382049003 28.043269224939582 28.552466340162912 15 21.657741354611137 33.92224075565709 23.228736524545386 21.191281365186388 16 20.629937770480627 28.135733458658642 30.4314615092227 20.80286726163803 17 20.538743167429903 29.8522938123565 29.074794805283137 20.53416821493046 18 20.482954281721852 27.194724773697164 32.55467210861729 19.767648835963694 19 23.366124480409884 26.67721085922655 28.068422605747717 21.88824205461585 20 23.51919212320121 27.782034839686947 29.1308659429437 19.567907094168138 21 21.82493262413639 26.916536331263046 27.75400862152538 23.504522423075183 22 22.923947140078628 29.594708032070628 27.76563103761963 19.715713790231113 23 20.813924020446926 29.18423693663143 29.196564586985314 20.805274455936328 24 23.1085732595605 26.809824042412366 29.919546293853866 20.162056404173267 25 21.792299105607015 28.280958714159322 29.378207053311456 20.548535126922207 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1026.0 1 1026.0 2 1596.0 3 2166.0 4 2166.0 5 2166.0 6 3234.0 7 4302.0 8 4302.0 9 4302.0 10 5164.5 11 6027.0 12 6027.0 13 6027.0 14 8861.0 15 11695.0 16 11695.0 17 11695.0 18 20866.0 19 30037.0 20 30037.0 21 30037.0 22 50057.5 23 70078.0 24 70078.0 25 70078.0 26 105010.0 27 139942.0 28 139942.0 29 139942.0 30 183901.5 31 227861.0 32 227861.0 33 227861.0 34 252031.5 35 276202.0 36 276202.0 37 276202.0 38 287382.0 39 298562.0 40 298562.0 41 298562.0 42 306020.5 43 313479.0 44 313479.0 45 313479.0 46 315615.5 47 317752.0 48 317752.0 49 317752.0 50 298295.5 51 278839.0 52 278839.0 53 278839.0 54 239752.0 55 200665.0 56 200665.0 57 200665.0 58 169359.0 59 138053.0 60 138053.0 61 138053.0 62 111876.5 63 85700.0 64 85700.0 65 85700.0 66 65738.0 67 45776.0 68 45776.0 69 45776.0 70 32987.0 71 20198.0 72 20198.0 73 20198.0 74 13975.5 75 7753.0 76 7753.0 77 7753.0 78 5293.5 79 2834.0 80 2834.0 81 2834.0 82 1883.5 83 933.0 84 933.0 85 933.0 86 621.5 87 310.0 88 310.0 89 310.0 90 207.0 91 104.0 92 104.0 93 104.0 94 81.5 95 59.0 96 59.0 97 59.0 98 49.5 99 40.0 100 40.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34518723444228394 2 0.2937034574762951 3 0.31591767917422764 4 0.3057983150250787 5 0.29076037547275774 6 0.24834774166835658 7 0.3539358480692374 8 0.31555483344776414 9 0.2606038639844573 10 0.23540624409115812 11 0.25048449983530835 12 0.24709793972164895 13 0.26451453459189733 14 0.25407264090811416 15 0.3460741906625281 16 0.40404887451303084 17 0.4201753512447423 18 0.11490114671344419 19 0.12901181385369173 20 0.1922276026420007 21 0.1906552711606588 22 0.15054066029052654 23 0.25318568468787 24 0.34917853743338256 25 0.3897366264136369 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2480393.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.76396551764508 #Duplication Level Percentage of deduplicated Percentage of total 1 81.96873741909043 53.08620483734876 2 11.856280713046111 15.357195105344774 3 3.1651591954727243 6.149647829803582 4 1.1856844972964495 3.0715851959085434 5 0.562422119742642 1.821234338468666 6 0.3052091238462826 1.1859931903470788 7 0.1956428632366274 0.8869425353901202 8 0.13772291926979766 0.7135585915662873 9 0.08837412730291767 0.5151113039968322 >10 0.46576836800379634 5.384078490020413 >50 0.03544330774871202 1.5416797101828752 >100 0.02698929079507142 3.5606679241412844 >500 0.0036269637963031428 1.587090745552121 >1k 0.0025638882008236497 3.24525794966342 >5k 3.752031513400463E-4 1.893752252265185 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 9609 0.3873982872875387 No Hit GTCCTAAAGTGTGTATTTCTCATTT 8746 0.3526054137388712 No Hit CTGTAGGACGTGGAATATGGCAAGA 8650 0.3487350593232605 No Hit CTTTAGGACGTGAAATATGGCGAGG 7772 0.3133374428971538 No Hit GTATCAACGCAGAGTACTTTTTTTT 6674 0.2690702642686058 No Hit GTCCTACAGTGTGCATTTCTCATTT 5309 0.21403866242164044 No Hit CTGAAGGACCTGGAATATGGCGAGA 4102 0.1653770188837011 No Hit CTGTAGGACCTGGAATATGGCGAGA 4092 0.1649738569654083 No Hit GGTATCAACGCAGAGTACTTTTTTT 3875 0.15622524333845483 No Hit TATCAACGCAGAGTACTTTTTTTTT 3845 0.15501575758357647 No Hit GTCCTTCAGTGTGCATTTCTCATTT 3400 0.13707505221954747 No Hit ATTTAGAAATGTCCACTGTAGGACG 3285 0.13243869015918042 No Hit TTTCTAAATTTTCCACCTTTTTCAG 3043 0.12268217173649498 No Hit GAATATGGCAAGAAAACTGAAAATC 2896 0.11675569153759102 No Hit GGAATATGGCGAGAAAACTGAAAAT 2526 0.10183870056075792 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.031619182927867E-5 2 0.0 0.0 0.0 0.0 4.031619182927867E-5 3 0.0 0.0 0.0 0.0 4.031619182927867E-5 4 0.0 0.0 0.0 0.0 4.031619182927867E-5 5 0.0 1.20948575487836E-4 0.0 0.0 4.031619182927867E-5 6 0.0 1.20948575487836E-4 0.0 0.0 4.031619182927867E-5 7 0.0 1.20948575487836E-4 0.0 0.0 4.031619182927867E-5 8 0.0 1.20948575487836E-4 0.0 0.0 4.031619182927867E-5 9 0.0 1.20948575487836E-4 0.0 0.0 4.031619182927867E-5 10 0.0 1.20948575487836E-4 0.0 0.0 4.031619182927867E-5 11 0.0 1.20948575487836E-4 0.0 0.0 4.031619182927867E-5 12 0.0 1.20948575487836E-4 0.0 0.0 4.031619182927867E-5 13 0.0 1.20948575487836E-4 0.0 0.0 4.031619182927867E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGA 145 0.0 13.768988 19 TAGGACC 1305 0.0 12.957895 4 GGTATCA 1345 0.0 12.85506 1 TCCAACG 290 0.0 12.780563 18 AAATGTC 1120 0.0 12.299146 7 GGCGAGG 2325 0.0 11.7766075 19 TGGCGAA 195 0.0 11.696611 18 GCCGGTT 215 0.0 11.480035 11 ATAGGAC 410 0.0 11.354385 3 TAGAAAT 1290 0.0 11.341123 4 AATGTCC 1235 0.0 11.288287 8 CACCTTT 1130 0.0 11.192337 14 TTTAGAA 1345 0.0 11.08993 2 ATTTAGA 1185 0.0 10.983157 1 TGTCCAC 1350 0.0 10.96759 10 CCACCTT 1170 0.0 10.800712 13 ATGTCCA 1320 0.0 10.778672 9 GAAATGT 1330 0.0 10.71432 6 AGGACCT 2840 0.0 10.704259 5 GACGTGA 2830 0.0 10.574239 7 >>END_MODULE