FastQCFastQC Report
Thu 26 May 2016
SRR1512266_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512266_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2480393
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT96090.3873982872875387No Hit
CTGTAGGACGTGGAATATGGCAAGA91350.3682884123604606No Hit
GTCCTAAAGTGTGTATTTCTCATTT88960.358652842513263No Hit
CTTTAGGACGTGAAATATGGCGAGG81070.32684336715996215No Hit
GTATCAACGCAGAGTACTTTTTTTT74970.30225049014410216No Hit
GTCCTACAGTGTGCATTTCTCATTT51990.20960388132041977No Hit
GGTATCAACGCAGAGTACTTTTTTT48110.1939611988906597No Hit
TATCAACGCAGAGTACTTTTTTTTT47390.19105843307895162No Hit
CTGAAGGACCTGGAATATGGCGAGA44850.1808181203543148No Hit
CTGTAGGACCTGGAATATGGCGAGA43810.17662523640406985No Hit
TTTCTAAATTTTCCACCTTTTTCAG35220.14199362762271947No Hit
ATTTAGAAATGTCCACTGTAGGACG34410.1387280160845479No Hit
GTCCTTCAGTGTGCATTTCTCATTT33190.13380944068137587No Hit
GAATATGGCAAGAAAACTGAAAATC31400.126592842343935No Hit
GGAATATGGCGAGAAAACTGAAAAT27110.10929719604917446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13550.013.4064761
TAGGACC16350.013.0775334
CTGATCG701.0873662E-412.2191389
TCCAACG2650.012.18000118
CGGAATA550.003071332612.08985711
GGCGAGG21850.012.03514619
GCCGGTT1800.011.61010111
AGGACCG1406.7666406E-1011.5400655
CCGGTTT1900.011.49912
TCTATAC2100.011.3131053
CGTTAGG600.005827483711.0960231
AACCGCT600.00585294111.0895277
ACCGCTC600.00585294111.0895278
CCGTGTA600.005859980411.0877369
AAATGTC11900.010.9430877
AGGACCT32500.010.8779465
TGTAGGA53750.010.8553092
GGACCTG30950.010.8101256
CTGTAGG53900.010.5696021
GTCGAGG1355.7498255E-810.54825219
Produced by FastQC (version 0.11.5)