FastQCFastQC Report
Thu 26 May 2016
SRR1512265_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512265_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2176158
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT71120.3268145051967734No Hit
CTGTAGGACGTGGAATATGGCAAGA64740.29749678102417193No Hit
GTCCTAAAGTGTGTATTTCTCATTT55420.2546690084083968No Hit
GTATCAACGCAGAGTACTTTTTTTT52150.23964252595629545No Hit
CTTTAGGACGTGAAATATGGCGAGG44620.20504025902530976No Hit
GTCCTACAGTGTGCATTTCTCATTT40230.18486709145199937No Hit
GGTATCAACGCAGAGTACTTTTTTT30850.14176360356187373No Hit
TATCAACGCAGAGTACTTTTTTTTT29040.13344619278563413No Hit
CTGAAGGACCTGGAATATGGCGAGA27520.1264614058354219No Hit
CTGTAGGACCTGGAATATGGCGAGA27100.12453139891496849No Hit
GTCCTTCAGTGTGCATTTCTCATTT24270.11152682847477068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2050.014.36793118
TAGGACC10750.014.3183494
GGTATCA11150.012.3557611
CCAACGT1353.6925485E-1011.96883819
TGTAGGA35050.011.9009872
GCCGGTT2100.011.75464911
GAACCGA658.0205913E-411.6931896
AGGACCT22100.011.6077455
GTAGGAC34700.011.4733713
GGACCTG21350.011.4364596
CCAACGA1251.819717E-811.40559919
GTGTAGG5000.011.4013851
GTCCTAC32200.011.3305691
GGCGAGG13000.011.18625919
GTCGAGA855.298305E-511.1819619
GTCCTAG3850.011.1052461
ACCGTTT600.005949750511.0650098
GGACGTG32950.011.0433216
CGGTTTC2250.010.97835213
AGGACGT33300.010.9562855
Produced by FastQC (version 0.11.5)