FastQCFastQC Report
Thu 26 May 2016
SRR1512265_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512265_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2176158
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA70850.3255737864621962No Hit
GTCCTACAGTGGACATTTCTAAATT69850.32097853188968817No Hit
GTATCAACGCAGAGTACTTTTTTTT61560.28288387148359634No Hit
GTCCTAAAGTGTGTATTTCTCATTT53910.24773017400390962No Hit
CTTTAGGACGTGAAATATGGCGAGG48240.22167508057778892No Hit
GTCCTACAGTGTGCATTTCTCATTT39340.18077731488246718No Hit
GGTATCAACGCAGAGTACTTTTTTT38160.17535491448690765No Hit
TATCAACGCAGAGTACTTTTTTTTT37020.17011632427424847No Hit
CTGAAGGACCTGGAATATGGCGAGA30440.1398795491871454No Hit
CTGTAGGACCTGGAATATGGCGAGA30200.13877668808974347No Hit
GTCCTTCAGTGTGCATTTCTCATTT22440.10311751260708092No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGATA350.002170995316.28524210
TCCAACG2150.014.13067818
TAGGACC10000.013.5877294
CGACTAA655.48424E-513.1462117
GGTATCA11600.012.8704061
CCAACGA1457.2759576E-1212.44076319
CCAACGT1251.4260877E-912.15266219
ACCGACT657.996724E-411.6973478
AGGACCT19450.011.578965
GTAGGAC33100.011.3675813
TGTAGGA34400.011.3246892
CGGTTTC2100.011.30893513
GGGCGAA600.005878207711.08301211
CTGTAGG33800.010.9160521
GACGTGG17800.010.838947
GTCCTAC31950.010.774281
GATATAC7350.010.7384421
GGACCTG19650.010.7369576
GGACGTG33450.010.6259136
AGGACGT34050.010.577015