FastQCFastQC Report
Thu 26 May 2016
SRR1512264_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512264_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1583245
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT79380.5013753398873831No Hit
GGTATCAACGCAGAGTACTTTTTTT49780.31441754118914No Hit
TATCAACGCAGAGTACTTTTTTTTT47460.29976409210198046No Hit
GTCCTACAGTGGACATTTCTAAATT25240.1594194202413398No Hit
GTCCTAAAGTGTGTATTTCTCATTT24070.15202953427928084No Hit
CTGTAGGACGTGGAATATGGCAAGA23780.15019785314338588No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23490.14836617200749094No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21890.13826034505082915No Hit
CTTTAGGACGTGAAATATGGCGAGG21310.13459698277903925No Hit
GAGTACTTTTTTTTTTTTTTTTTTT17330.10945873822434304No Hit
GTACTGGTTCACTATCGGTCAGTCA16140.10194252942532583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13250.013.6244391
TAGGACC4450.012.5967684
GTATAGG1700.012.295741
GTGGTAT3950.011.5458281
CTAATAG752.0719192E-411.4011433
TAGAACG600.005871336411.0844454
GGCGAGG7450.010.7196319
AGGACCT8850.010.4131435
CGTGCGC750.002669186810.12342210
GTATCAA28250.010.0898281
GACGTGG6600.010.0767687
CGCGCCT856.627459E-410.0531312
GCGACTA1909.822543E-1110.00990116
GTAGGAC12950.09.9778343
GTCCTAA6550.09.8638971
TGTAGGA14100.09.8378662
GGACGTG15700.09.803226
GACGAAA1651.0086296E-89.79539119
AGCGTAC1651.0191798E-89.7891711
CGACTAA1951.6007107E-109.75354717
Produced by FastQC (version 0.11.5)