##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512264_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1583245 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.837429456590737 31.0 30.0 34.0 16.0 34.0 2 29.701719569618096 31.0 30.0 34.0 19.0 34.0 3 30.340237297449224 31.0 31.0 34.0 26.0 34.0 4 33.98853178124674 37.0 35.0 37.0 30.0 37.0 5 34.156348512074885 37.0 35.0 37.0 30.0 37.0 6 34.31137252920426 37.0 35.0 37.0 31.0 37.0 7 34.25224207245246 37.0 35.0 37.0 31.0 37.0 8 34.25213469804105 37.0 35.0 37.0 31.0 37.0 9 35.79775587480144 39.0 37.0 39.0 30.0 39.0 10 35.73350365862517 39.0 37.0 39.0 30.0 39.0 11 35.7568335917688 39.0 37.0 39.0 30.0 39.0 12 35.69384207750538 39.0 37.0 39.0 30.0 39.0 13 35.65763732081895 39.0 37.0 39.0 30.0 39.0 14 36.85916835360288 40.0 38.0 41.0 31.0 41.0 15 36.77484975477579 40.0 38.0 41.0 30.0 41.0 16 36.69696225157825 40.0 37.0 41.0 30.0 41.0 17 36.66639907279038 40.0 37.0 41.0 30.0 41.0 18 36.44237246920091 40.0 37.0 41.0 29.0 41.0 19 36.55317528241049 40.0 37.0 41.0 30.0 41.0 20 36.48235680516913 40.0 37.0 41.0 30.0 41.0 21 36.42636673414412 40.0 37.0 41.0 29.0 41.0 22 36.344035193542375 40.0 37.0 41.0 29.0 41.0 23 36.21355191394888 40.0 37.0 41.0 29.0 41.0 24 36.063278898717506 40.0 37.0 41.0 28.0 41.0 25 35.945479695182996 40.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7636728988473358 1101 2 0.021273157814473365 1101 3 -0.5045447544840229 1101 4 -0.35346077136910026 1101 5 -0.7245259871175165 1101 6 -0.7356546349743809 1101 7 -0.7754156174456313 1101 8 -0.8506373692551676 1101 9 -0.876015676417687 1101 10 -0.9274759367159007 1101 11 -1.0229309686269161 1101 12 -1.0565483116433754 1101 13 -1.0315878607257218 1101 14 -1.1892583247358246 1101 15 -1.3208676180087764 1101 16 -1.3513908943028525 1101 17 -1.5350888203422244 1101 18 -1.259996790983422 1101 19 -1.544729806592315 1101 20 -1.7594683452553497 1101 21 -1.8349473297968615 1101 22 -1.7282493386012803 1101 23 -1.6034738512700883 1101 24 -1.862981983551002 1101 25 -1.9502008443091157 1102 1 0.9023251251654258 1102 2 0.0938914717610615 1102 3 -0.36234471421890646 1102 4 -0.5000675561291956 1102 5 -0.5684955825585902 1102 6 -0.6775748414693723 1102 7 -0.6465817497349988 1102 8 -0.9017098895437812 1102 9 -1.0648362161988274 1102 10 -1.1173193687104899 1102 11 -0.9955432164314573 1102 12 -1.0843334401005293 1102 13 -0.9970605230851319 1102 14 -1.2021101939427652 1102 15 -1.4439307384146645 1102 16 -1.5779340461120128 1102 17 -1.4509703318841574 1102 18 -1.5163840429419508 1102 19 -1.459835467357827 1102 20 -1.6645736631519625 1102 21 -1.7390077767044971 1102 22 -1.8255142118584686 1102 23 -2.0010136350516916 1102 24 -1.825140686135093 1102 25 -1.7787189209728425 1103 1 0.8704889226270431 1103 2 -0.020397235899316968 1103 3 -0.38407827726603117 1103 4 -0.5064101844451869 1103 5 -0.6882264116568493 1103 6 -0.7402311621462729 1103 7 -0.7492345448817446 1103 8 -0.8865060407291523 1103 9 -1.083127398382267 1103 10 -1.0053031313159195 1103 11 -1.0647966022707962 1103 12 -1.0825074815554387 1103 13 -1.135791797863618 1103 14 -1.2954602458279254 1103 15 -1.4039658606773884 1103 16 -1.576217844637938 1103 17 -1.6870469822313865 1103 18 -1.4435618893086613 1103 19 -1.7385776845836958 1103 20 -1.7907518886794591 1103 21 -1.8388043935259262 1103 22 -1.9459410005661297 1103 23 -1.7716562793323476 1103 24 -1.64545588848722 1103 25 -1.883605106216173 1104 1 0.9178590378655684 1104 2 0.15994381219184461 1104 3 -0.39901180588252316 1104 4 -0.3773028001665608 1104 5 -0.5360709201226967 1104 6 -0.633958227104749 1104 7 -0.6566381372335073 1104 8 -0.6359310552144422 1104 9 -0.7866159389088878 1104 10 -0.8015703527155225 1104 11 -0.6979936665854822 1104 12 -0.9122232479881944 1104 13 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-0.8274917470938519 2314 18 -1.8769832815809835 2314 19 -1.288315020229419 2314 20 -1.9632558210824627 2314 21 -1.268386651330239 2314 22 -0.9543301269676832 2314 23 -0.6894438489056327 2314 24 -0.5543274904877009 2314 25 -0.6318657741196958 2315 1 -2.6530139577373575 2315 2 -1.1955373466482122 2315 3 -0.5426864360656545 2315 4 -0.45002091101152786 2315 5 -0.2802370288139997 2315 6 -0.24939976394147578 2315 7 -0.3114504159128657 2315 8 -0.2815198285199827 2315 9 -0.22260569517143836 2315 10 -0.3190861773571001 2315 11 -0.4242415180927068 2315 12 -0.46995333517040905 2315 13 -0.507789185440096 2315 14 -0.621636952834578 2315 15 -1.0981158182534188 2315 16 -0.8609184207108171 2315 17 -0.7173921235701997 2315 18 -0.6249947031344121 2315 19 -0.7474518305221594 2315 20 -0.7225790779796881 2315 21 -0.7784375936331074 2315 22 -0.5963049535871647 2315 23 -0.5293199465497835 2315 24 -0.33926003487224676 2315 25 -0.3647482089714913 2316 1 1.4194019165237535 2316 2 0.7001143594406543 2316 3 0.2548554030456103 2316 4 0.030073854856716764 2316 5 -0.17994699908717848 2316 6 -0.2573601751283121 2316 7 -0.19079257175811648 2316 8 -0.2514921167420354 2316 9 -0.307577851339488 2316 10 -0.3710737858003341 2316 11 -0.4259421780692918 2316 12 -0.38521222460599347 2316 13 -0.3978472794214625 2316 14 -0.4211421904877781 2316 15 -0.24310446649969464 2316 16 -0.24466501787468786 2316 17 -0.2089476121749172 2316 18 -0.05290681189995894 2316 19 -0.32072664273647433 2316 20 -0.21136378006095669 2316 21 -0.24007551328362098 2316 22 -0.42230785486260913 2316 23 -0.38890799673812637 2316 24 -0.3969112929556644 2316 25 -0.2880205268323337 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49961.0 3 2.0 4 371.0 5 1983.0 6 2416.0 7 2143.0 8 2071.0 9 1904.0 10 1841.0 11 1918.0 12 1950.0 13 1903.0 14 1856.0 15 1982.0 16 2145.0 17 2170.0 18 2502.0 19 2954.0 20 3576.0 21 4447.0 22 5447.0 23 6583.0 24 7555.0 25 8882.0 26 10093.0 27 11176.0 28 12840.0 29 15554.0 30 19806.0 31 24713.0 32 33423.0 33 44797.0 34 62228.0 35 89616.0 36 151566.0 37 256329.0 38 732542.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.24796710606797 19.367842720658285 12.267466661426896 24.11672351184685 2 15.12086901269348 20.244633129493508 41.057834297417784 23.57666356039523 3 18.770264473356555 24.995928888291864 29.692249300307378 26.541557338044207 4 12.46547013832219 16.747806561814155 38.16576107616591 32.620962223697745 5 13.280467556786471 37.77145286075096 35.33573336694985 13.612346215512714 6 31.614113672010298 36.73542658822501 18.140740332674167 13.509719407090524 7 27.81421648331099 31.841955962942187 22.270694469536334 18.073133084210493 8 25.318034959926045 36.02810024287055 20.82033598887852 17.83352880832489 9 26.82779479151627 14.919803231681522 20.355882658398748 37.89651931840346 10 15.747912353674828 28.40262609763664 34.000810367641 21.848651181047526 11 34.07839482095729 22.411618400190466 24.67922253572496 18.83076424312728 12 23.646509519262672 24.67004870438238 31.399763443093377 20.283678333261573 13 29.119098598970396 21.95550117697126 25.940991214354426 22.984409009703917 14 21.334649610784187 22.261574049155236 27.817674887490572 28.586101452570002 15 23.23983254157146 30.494864375786346 23.889954078867724 22.37534900377447 16 22.2361463265521 27.88479466667343 27.59103552633749 22.288023480436987 17 21.7924305700034 28.240489371742893 27.766797416133436 22.20028264212027 18 21.62325380509395 26.90147195432812 29.962241529006235 21.513032711571697 19 23.37148903363558 26.602422409469543 28.245716891407724 21.78037166548715 20 23.66645930540517 26.56936930323452 28.628067939396505 21.136103451963802 21 23.20614812531919 26.224014975423827 27.87084329785867 22.698993601398314 22 23.456757322665393 27.399733427968304 27.721024208460086 21.42248504090622 23 22.468534780813627 27.073196369816692 28.444900088905552 22.013368760464132 24 23.19382211384567 26.30235156979438 28.74608510123115 21.7577412151288 25 22.816460211221834 26.750157734707656 28.642783993811094 21.790598060259413 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 877.0 1 877.0 2 1292.0 3 1707.0 4 1707.0 5 1707.0 6 2876.5 7 4046.0 8 4046.0 9 4046.0 10 4748.5 11 5451.0 12 5451.0 13 5451.0 14 6847.5 15 8244.0 16 8244.0 17 8244.0 18 14032.5 19 19821.0 20 19821.0 21 19821.0 22 30333.5 23 40846.0 24 40846.0 25 40846.0 26 58538.5 27 76231.0 28 76231.0 29 76231.0 30 96384.5 31 116538.0 32 116538.0 33 116538.0 34 130455.5 35 144373.0 36 144373.0 37 144373.0 38 154862.0 39 165351.0 40 165351.0 41 165351.0 42 175408.5 43 185466.0 44 185466.0 45 185466.0 46 195175.0 47 204884.0 48 204884.0 49 204884.0 50 199661.0 51 194438.0 52 194438.0 53 194438.0 54 174977.0 55 155516.0 56 155516.0 57 155516.0 58 134479.5 59 113443.0 60 113443.0 61 113443.0 62 94256.5 63 75070.0 64 75070.0 65 75070.0 66 57610.5 67 40151.0 68 40151.0 69 40151.0 70 29540.0 71 18929.0 72 18929.0 73 18929.0 74 13243.5 75 7558.0 76 7558.0 77 7558.0 78 5208.0 79 2858.0 80 2858.0 81 2858.0 82 1876.0 83 894.0 84 894.0 85 894.0 86 613.5 87 333.0 88 333.0 89 333.0 90 228.5 91 124.0 92 124.0 93 124.0 94 88.0 95 52.0 96 52.0 97 52.0 98 48.0 99 44.0 100 44.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35168277809183035 2 0.2979955723845646 3 0.3190914861565961 4 0.30955411196624655 5 0.29407956443885813 6 0.2515087683839204 7 0.36065169951586773 8 0.31820722629788817 9 0.26079349690035336 10 0.234644669649991 11 0.24992973292194196 12 0.24765592185669308 13 0.26477266626453894 14 0.2554247763296268 15 0.3499142583744146 16 0.4069490192610745 17 0.4227393738808586 18 0.11861714390381779 19 0.13213368745835294 20 0.19403187756790644 21 0.19302129487224023 22 0.1537348925782175 23 0.25523529207418943 24 0.3548408490157872 25 0.39349563712501856 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1583245.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.40367659898077 #Duplication Level Percentage of deduplicated Percentage of total 1 83.5240295188931 57.968747329729794 2 11.005379628865631 15.276276172216027 3 2.846557156485043 5.926845969276067 4 1.0433075714191964 2.896375251201838 5 0.4980079508253197 1.7281791381400802 6 0.2628312687893003 1.094487382749143 7 0.1695531259449916 0.8237327223602717 8 0.11738895888494559 0.6517780270993452 9 0.07808040041846757 0.4877160173426021 >10 0.39892175662225365 4.82136106842366 >50 0.031109886862113606 1.5205206684121557 >100 0.02035944759515188 2.8603088769548664 >500 0.0030126497350488712 1.3849292911324702 >1k 0.0013693862432040324 2.0557880766805803 >5k 9.129241621360216E-5 0.5029540082811579 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7938 0.5013753398873831 No Hit GGTATCAACGCAGAGTACTTTTTTT 4978 0.31441754118914 No Hit TATCAACGCAGAGTACTTTTTTTTT 4746 0.29976409210198046 No Hit GTCCTACAGTGGACATTTCTAAATT 2524 0.1594194202413398 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2407 0.15202953427928084 No Hit CTGTAGGACGTGGAATATGGCAAGA 2378 0.15019785314338588 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2349 0.14836617200749094 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2189 0.13826034505082915 No Hit CTTTAGGACGTGAAATATGGCGAGG 2131 0.13459698277903925 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1733 0.10945873822434304 No Hit GTACTGGTTCACTATCGGTCAGTCA 1614 0.10194252942532583 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 6.31614184791362E-5 0.0 11 0.0 0.0 0.0 1.263228369582724E-4 0.0 12 0.0 0.0 0.0 1.263228369582724E-4 0.0 13 0.0 0.0 0.0 1.263228369582724E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1325 0.0 13.624439 1 TAGGACC 445 0.0 12.596768 4 GTATAGG 170 0.0 12.29574 1 GTGGTAT 395 0.0 11.545828 1 CTAATAG 75 2.0719192E-4 11.401143 3 TAGAACG 60 0.0058713364 11.084445 4 GGCGAGG 745 0.0 10.71963 19 AGGACCT 885 0.0 10.413143 5 CGTGCGC 75 0.0026691868 10.123422 10 GTATCAA 2825 0.0 10.089828 1 GACGTGG 660 0.0 10.076768 7 CGCGCCT 85 6.627459E-4 10.05313 12 GCGACTA 190 9.822543E-11 10.009901 16 GTAGGAC 1295 0.0 9.977834 3 GTCCTAA 655 0.0 9.863897 1 TGTAGGA 1410 0.0 9.837866 2 GGACGTG 1570 0.0 9.80322 6 GACGAAA 165 1.0086296E-8 9.795391 19 AGCGTAC 165 1.0191798E-8 9.789171 1 CGACTAA 195 1.6007107E-10 9.753547 17 >>END_MODULE