FastQCFastQC Report
Thu 26 May 2016
SRR1512264_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512264_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1583245
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT93940.5933383651930054No Hit
GGTATCAACGCAGAGTACTTTTTTT61190.3864847196738344No Hit
TATCAACGCAGAGTACTTTTTTTTT59540.37606308562477697No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28320.17887313713291372No Hit
GTCCTACAGTGGACATTTCTAAATT26830.16946208577952243No Hit
GTCCTAAAGTGTGTATTTCTCATTT25810.16301962109465054No Hit
CTGTAGGACGTGGAATATGGCAAGA25370.16024051868156855No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23540.14868197909988665No Hit
CTTTAGGACGTGAAATATGGCGAGG23100.14590287668680463No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19730.12461747865933573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14600.014.7850831
TATACTG1950.011.6964135
CGTCGGT907.513074E-611.60381519
TTAGGAC9450.011.1622943
TTCCGAA700.001499664210.849977518
TGGCGAA803.7938292E-410.68044718
GGCGAGG7500.010.63331419
TTAGACT1803.45608E-1110.5592614
CCTATAC1355.658694E-810.55892853
GACGTGA8800.010.4769247
TAGGACC4400.010.3672754
ATTAGAC1106.0212806E-610.3669473
ACGTGAA9650.010.2435568
GATATAC2600.010.2408371
AGGACGT14950.010.2343625
GTATCAA30500.010.2264471
CGTGAAA9650.010.1437789
TAGTGGT750.002639917410.1368914
AGTACCG750.002639917410.1368915
TTTAGGA10350.010.09952452
Produced by FastQC (version 0.11.5)