FastQCFastQC Report
Thu 26 May 2016
SRR1512263_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512263_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1455493
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56620.3890090848942592No Hit
GGTATCAACGCAGAGTACTTTTTTT35030.24067446562779762No Hit
TATCAACGCAGAGTACTTTTTTTTT31900.21916972462251622No Hit
GTCCTAAAGTGTGTATTTCTCATTT27950.1920311537053081No Hit
GTCCTACAGTGGACATTTCTAAATT27820.19113798554853922No Hit
CTGTAGGACGTGGAATATGGCAAGA25710.1766411793117521No Hit
CTTTAGGACGTGAAATATGGCGAGG23600.162144373074965No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16050.11027191473954186No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15150.10408844288498811No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4750.013.9989054
CGAAATC852.6943417E-713.41349513
TACGGCG500.001488673313.3127516
GGTATCA8850.012.9912741
GGATATA1052.712186E-711.7642351
TGTCGAG658.007202E-411.69502718
GTGTAGG2250.011.4022591
GTATTAA1803.6379788E-1211.0855291
GTCCTAA7350.010.9885711
TCGAACT1053.4397362E-610.86379519
TAGACAG2100.010.8562955
TGGACCG700.001492268510.8562955
TGGTATC3250.010.8160332
GTATCAA20800.010.5525711
AGGACCT10700.010.4758175
GTGGTAT3450.010.465841
CCAACGA1002.3819039E-510.45640419
GATATAC2200.010.3656881
TGTAGGA14750.010.3057022
TTAGAAC1856.002665E-1110.2708863
Produced by FastQC (version 0.11.5)