##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512263_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1455493 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.20870797729704 31.0 30.0 34.0 16.0 34.0 2 30.076655126476048 33.0 30.0 34.0 25.0 34.0 3 30.743539130727527 33.0 31.0 34.0 27.0 34.0 4 34.39997306754481 37.0 35.0 37.0 32.0 37.0 5 34.56946684044513 37.0 35.0 37.0 32.0 37.0 6 34.722634873544564 37.0 35.0 37.0 32.0 37.0 7 34.6558197119464 37.0 35.0 37.0 32.0 37.0 8 34.65663730433606 37.0 35.0 37.0 32.0 37.0 9 36.25496309497881 39.0 37.0 39.0 32.0 39.0 10 36.18889613347505 39.0 37.0 39.0 32.0 39.0 11 36.21607386638067 39.0 37.0 39.0 32.0 39.0 12 36.15394715055311 39.0 37.0 39.0 32.0 39.0 13 36.132000634836444 39.0 37.0 39.0 32.0 39.0 14 37.39048556056264 40.0 38.0 41.0 32.0 41.0 15 37.32702596302421 40.0 38.0 41.0 32.0 41.0 16 37.25304759280876 40.0 38.0 41.0 32.0 41.0 17 37.21590622558817 40.0 38.0 41.0 32.0 41.0 18 36.99682719188618 40.0 38.0 41.0 31.0 41.0 19 37.10257280522819 40.0 38.0 41.0 31.0 41.0 20 37.03301836559846 40.0 38.0 41.0 31.0 41.0 21 36.98438604651482 40.0 38.0 41.0 31.0 41.0 22 36.91332490090986 40.0 38.0 41.0 31.0 41.0 23 36.79662080133673 40.0 38.0 41.0 31.0 41.0 24 36.67389331312483 40.0 38.0 41.0 30.0 41.0 25 36.56320504461375 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8596922853496523 1101 2 0.04733159739638282 1101 3 -0.5055682879995196 1101 4 -0.4291548230831026 1101 5 -0.4623320095313588 1101 6 -0.7020556407914995 1101 7 -0.8640884870225349 1101 8 -0.8014066925439067 1101 9 -1.005541499736161 1101 10 -1.0695187861543118 1101 11 -0.921362251372436 1101 12 -1.1913648614556038 1101 13 -1.2121770334934396 1101 14 -1.490838420038635 1101 15 -1.583428849122754 1101 16 -1.6088691644857747 1101 17 -1.717753366203766 1101 18 -1.5910983878702325 1101 19 -1.8740544290024843 1101 20 -1.6447601739077555 1101 21 -1.9183827291421807 1101 22 -1.8182973129430664 1101 23 -2.033093321137059 1101 24 -2.1119743379988165 1101 25 -2.1499386218873227 1102 1 0.9473903699576169 1102 2 0.15545872588316456 1102 3 -0.30882671828358 1102 4 -0.3331091332073157 1102 5 -0.5094047865545264 1102 6 -0.4872295615379727 1102 7 -0.5185851306572573 1102 8 -0.6498697528403738 1102 9 -0.8796463049488565 1102 10 -0.8845825669374037 1102 11 -0.9221043919657532 1102 12 -1.1521254079230516 1102 13 -0.9737328712067779 1102 14 -1.0027672965913226 1102 15 -1.208086814830743 1102 16 -1.501913811033937 1102 17 -1.6688395096317876 1102 18 -1.443585641556325 1102 19 -1.453774010096602 1102 20 -1.5567008617165712 1102 21 -1.4735211176931315 1102 22 -1.5298885844247891 1102 23 -1.8922435996858482 1102 24 -1.6842232995925528 1102 25 -1.645425737775426 1103 1 1.3724248217997541 1103 2 0.6683630458239591 1103 3 0.04253747978901501 1103 4 0.02864872904698501 1103 5 -0.06758155474640404 1103 6 -0.12412588343321573 1103 7 -0.1005809893204983 1103 8 -0.22901079310810957 1103 9 -0.3138443538242228 1103 10 -0.307979459274506 1103 11 -0.42777346927307747 1103 12 -0.2513642653315671 1103 13 -0.48293513020398393 1103 14 -0.6965190013941793 1103 15 -0.7135338823326336 1103 16 -0.658509470931989 1103 17 -0.7826688704285516 1103 18 -0.5184000997077121 1103 19 -0.8425103331303561 1103 20 -0.9056841469398762 1103 21 -0.9296345703962672 1103 22 -1.1599932089174985 1103 23 -1.0096142066312481 1103 24 -1.2754724130950663 1103 25 -1.052173317844833 1104 1 1.4460808266608431 1104 2 0.6554213103603992 1104 3 0.3071122944143134 1104 4 0.2556241175000693 1104 5 -0.027385283713286412 1104 6 0.009473195518360455 1104 7 -0.11926328700737088 1104 8 -0.020491552059318963 1104 9 -0.13963508029203098 1104 10 -0.12736487874901314 1104 11 0.07016696411309908 1104 12 -0.06572595044813312 1104 13 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-0.22118901225145038 2314 9 -0.2633306323383522 2314 10 -0.30036790546305525 2314 11 -1.3085859968028117 2314 12 -0.7670336883083806 2314 13 -0.6409324339587101 2314 14 -0.4940786992011468 2314 15 -0.5886527607212955 2314 16 -0.5729145283940511 2314 17 -0.708873755037267 2314 18 -2.028951495214862 2314 19 -1.6747149474471499 2314 20 -2.051498292884162 2314 21 -1.3200111771315406 2314 22 -1.037815840693149 2314 23 -0.8113834507482238 2314 24 -0.756010978078578 2314 25 -0.6929657903818338 2315 1 -2.9508586620776995 2315 2 -1.5767893831066644 2315 3 -0.874570104592209 2315 4 -0.8225621054490091 2315 5 -0.6930749845160733 2315 6 -0.6011619114638123 2315 7 -0.6273237643385485 2315 8 -0.7136254205862755 2315 9 -0.7684147438393722 2315 10 -0.8432266060717097 2315 11 -1.0595803910215693 2315 12 -0.9390248558572978 2315 13 -0.9009891361137861 2315 14 -1.0111215628582357 2315 15 -1.3784080839830537 2315 16 -1.2015389559203697 2315 17 -1.1950680937434228 2315 18 -1.0067068036071802 2315 19 -1.0822777956400174 2315 20 -1.001601387711574 2315 21 -0.9841178208001367 2315 22 -1.1823549126414434 2315 23 -1.0281155429784476 2315 24 -0.9757820133885602 2315 25 -0.940809101113615 2316 1 1.3494527158760974 2316 2 0.45643680273129306 2316 3 -0.01927914363169947 2316 4 -0.24131149911281824 2316 5 -0.5549530262486044 2316 6 -0.6529670164643804 2316 7 -0.6057381958403312 2316 8 -0.5700657615145204 2316 9 -0.6329463980905246 2316 10 -0.6874769307047544 2316 11 -0.6587344515156346 2316 12 -0.6512635346850999 2316 13 -0.6279041338752052 2316 14 -0.7382624938067224 2316 15 -0.7914915090990462 2316 16 -0.7403174952247014 2316 17 -0.56442216347196 2316 18 -0.5471659641733027 2316 19 -0.6720998557460263 2316 20 -0.6402340950552059 2316 21 -0.5825930441523397 2316 22 -0.6042642581822548 2316 23 -0.7516368738208925 2316 24 -0.7572034573791768 2316 25 -0.7266931794903826 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 36865.0 3 1.0 4 328.0 5 1488.0 6 1867.0 7 1726.0 8 1609.0 9 1542.0 10 1459.0 11 1490.0 12 1473.0 13 1485.0 14 1500.0 15 1572.0 16 1556.0 17 1692.0 18 2007.0 19 2413.0 20 2734.0 21 3482.0 22 4143.0 23 5155.0 24 5986.0 25 7058.0 26 7970.0 27 8967.0 28 10076.0 29 12601.0 30 15689.0 31 20459.0 32 27327.0 33 37671.0 34 52251.0 35 77598.0 36 135172.0 37 231300.0 38 727781.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.398824310544185 19.215889871847644 11.92602229893759 24.459263518670586 2 15.091055307132063 20.052771903130687 41.399482204126244 23.45669058561101 3 18.8433417054255 24.82231130397863 29.701306542638395 26.633040447957473 4 12.45596974757252 16.60382455852586 38.184937053365594 32.755268640536016 5 13.197043313242176 37.80029065220342 35.68110671097884 13.32155932357556 6 31.78414976856954 36.86446164866652 18.15358441701565 13.197804165748291 7 27.843656141144415 31.64441404493917 22.394292002570474 18.11763781134594 8 25.121602387590595 36.68170398425734 20.660240482205076 17.53645314594699 9 26.83224893138474 14.82015230996559 20.111184208260283 38.236414550389384 10 15.441798322337158 28.41613751876696 34.4714948829906 21.67056927590529 11 34.54998057589041 21.859906709170108 24.985397477896466 18.604715237043017 12 23.876204245224862 24.21962980845725 31.905033808752048 19.99913213756584 13 29.446915335692164 21.74303925134751 25.69552373708982 23.114521675870506 14 21.242845540831993 22.125455581218652 27.612199898465057 29.019498979484293 15 23.150717704998737 30.820670621013562 23.495935912277204 22.532675761710493 16 22.327740176562827 27.5491542658542 27.872560740468373 22.250544817114605 17 21.882716922497345 28.263522963353438 27.716373968403353 22.137386145745864 18 21.746044957376544 26.74378532608509 29.920476111535677 21.589693605002694 19 23.698317258080053 26.34536798800204 27.70188087342974 22.254433880488175 20 24.006911673086123 26.61386533940514 28.227851151199733 21.151371836309004 21 23.340118498305547 26.18403652837275 27.36089666058365 23.11494831273805 22 23.74964133919301 27.507856133839482 27.31512540570816 21.427377121259347 23 22.508479105995026 27.24128050342884 28.09793884022912 22.152301550347016 24 23.494952030151296 26.122709589694722 28.498930555766243 21.883407824387742 25 22.974243512247075 26.853271692696318 28.31316699466715 21.859317800389462 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 745.0 1 745.0 2 1061.5 3 1378.0 4 1378.0 5 1378.0 6 2296.0 7 3214.0 8 3214.0 9 3214.0 10 3732.0 11 4250.0 12 4250.0 13 4250.0 14 5665.5 15 7081.0 16 7081.0 17 7081.0 18 11849.0 19 16617.0 20 16617.0 21 16617.0 22 26035.5 23 35454.0 24 35454.0 25 35454.0 26 51644.0 27 67834.0 28 67834.0 29 67834.0 30 87668.0 31 107502.0 32 107502.0 33 107502.0 34 120731.0 35 133960.0 36 133960.0 37 133960.0 38 143725.5 39 153491.0 40 153491.0 41 153491.0 42 163100.5 43 172710.0 44 172710.0 45 172710.0 46 180585.5 47 188461.0 48 188461.0 49 188461.0 50 183621.0 51 178781.0 52 178781.0 53 178781.0 54 161128.0 55 143475.0 56 143475.0 57 143475.0 58 124047.0 59 104619.0 60 104619.0 61 104619.0 62 86797.0 63 68975.0 64 68975.0 65 68975.0 66 53622.5 67 38270.0 68 38270.0 69 38270.0 70 28064.0 71 17858.0 72 17858.0 73 17858.0 74 12414.0 75 6970.0 76 6970.0 77 6970.0 78 4747.0 79 2524.0 80 2524.0 81 2524.0 82 1684.5 83 845.0 84 845.0 85 845.0 86 563.0 87 281.0 88 281.0 89 281.0 90 205.0 91 129.0 92 129.0 93 129.0 94 83.0 95 37.0 96 37.0 97 37.0 98 34.5 99 32.0 100 32.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3510837908529962 2 0.2983181643608042 3 0.31886103196648835 4 0.3093110032133442 5 0.2936462078484747 6 0.2509802520520538 7 0.3556870421224973 8 0.3200290210945707 9 0.26437777440358695 10 0.23861330834294633 11 0.25345364079387533 12 0.2507741363235687 13 0.26966807810137183 14 0.259430996919944 15 0.3508089698816827 16 0.4070098585152934 17 0.42336170630844666 18 0.1178981966934915 19 0.13143312953068134 20 0.19773368886006323 21 0.19333655331904723 22 0.14909037693757374 23 0.25345364079387533 24 0.3577481994073486 25 0.39849040840457495 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1455493.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.66670820075602 #Duplication Level Percentage of deduplicated Percentage of total 1 82.33405158149165 56.53608294932282 2 11.701541872692674 16.070127225422322 3 3.1377723592059694 6.463814969699823 4 1.1279308957913043 3.098052067676754 5 0.5484191728049345 1.8829069655348216 6 0.2954322627572026 1.2171816587902748 7 0.18439462192623013 0.886324018831802 8 0.11752970438383681 0.645630233267683 9 0.09229916978313252 0.5704092142803358 >10 0.4078060457727707 4.962928856230063 >50 0.02623661050443119 1.2367225831686546 >100 0.02265839150079872 3.0444231033199363 >500 0.0025175072981218896 1.1662430102949939 >1k 0.0013091037950233827 1.8276395710368185 >5k 1.007002919248756E-4 0.39151357312286583 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5662 0.3890090848942592 No Hit GGTATCAACGCAGAGTACTTTTTTT 3503 0.24067446562779762 No Hit TATCAACGCAGAGTACTTTTTTTTT 3190 0.21916972462251622 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2795 0.1920311537053081 No Hit GTCCTACAGTGGACATTTCTAAATT 2782 0.19113798554853922 No Hit CTGTAGGACGTGGAATATGGCAAGA 2571 0.1766411793117521 No Hit CTTTAGGACGTGAAATATGGCGAGG 2360 0.162144373074965 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1605 0.11027191473954186 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1515 0.10408844288498811 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.870524282837499E-5 2 0.0 0.0 0.0 0.0 6.870524282837499E-5 3 0.0 0.0 0.0 0.0 6.870524282837499E-5 4 0.0 0.0 0.0 0.0 6.870524282837499E-5 5 0.0 0.0 0.0 0.0 6.870524282837499E-5 6 0.0 0.0 0.0 0.0 6.870524282837499E-5 7 0.0 0.0 0.0 0.0 6.870524282837499E-5 8 0.0 0.0 0.0 0.0 6.870524282837499E-5 9 0.0 0.0 0.0 0.0 6.870524282837499E-5 10 0.0 0.0 0.0 0.0 6.870524282837499E-5 11 0.0 0.0 0.0 0.0 1.3741048565674998E-4 12 0.0 0.0 0.0 0.0 2.7482097131349996E-4 13 0.0 0.0 0.0 0.0 2.7482097131349996E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 475 0.0 13.998905 4 CGAAATC 85 2.6943417E-7 13.413495 13 TACGGCG 50 0.0014886733 13.31275 16 GGTATCA 885 0.0 12.991274 1 GGATATA 105 2.712186E-7 11.764235 1 TGTCGAG 65 8.007202E-4 11.695027 18 GTGTAGG 225 0.0 11.402259 1 GTATTAA 180 3.6379788E-12 11.085529 1 GTCCTAA 735 0.0 10.988571 1 TCGAACT 105 3.4397362E-6 10.863795 19 TAGACAG 210 0.0 10.856295 5 TGGACCG 70 0.0014922685 10.856295 5 TGGTATC 325 0.0 10.816033 2 GTATCAA 2080 0.0 10.552571 1 AGGACCT 1070 0.0 10.475817 5 GTGGTAT 345 0.0 10.46584 1 CCAACGA 100 2.3819039E-5 10.456404 19 GATATAC 220 0.0 10.365688 1 TGTAGGA 1475 0.0 10.305702 2 TTAGAAC 185 6.002665E-11 10.270886 3 >>END_MODULE