FastQCFastQC Report
Thu 26 May 2016
SRR1512263_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512263_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1455493
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60940.41868974979611717No Hit
GGTATCAACGCAGAGTACTTTTTTT40110.27557672898461205No Hit
TATCAACGCAGAGTACTTTTTTTTT38940.26753821557369223No Hit
GTCCTACAGTGGACATTTCTAAATT29680.203917160714617No Hit
GTCCTAAAGTGTGTATTTCTCATTT27950.1920311537053081No Hit
CTGTAGGACGTGGAATATGGCAAGA27420.18838977583540423No Hit
CTTTAGGACGTGAAATATGGCGAGG25980.17849622086811823No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19670.1351432126434136No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16480.11322624018116198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9900.014.8895821
GTACTAG852.6638554E-713.426051
TAGGACC4050.013.3772444
ATAGGAC1600.013.0687683
TCCAACG1103.8549842E-812.08133318
AGGACCG951.0323147E-612.0069965
GGCGAGG7150.011.01999519
TAAGTAG951.3511593E-511.0064135
GCACCGT803.7501048E-410.6937316
TAGAAAT5000.010.4553744
GTATAAG1659.113137E-1010.3746761
GAACAGT2850.010.3393586
GTATCAA21400.010.3100361
TTAGGAC10150.010.3005073
AGGACCT9900.010.27366355
GTCCTAA8850.010.2086031
TGGACTA856.557168E-410.0646885
TGGCGAG15650.010.00804118
TAGTACA1054.0805993E-59.9574994
CACCTTT3250.09.93297914
Produced by FastQC (version 0.11.5)