FastQCFastQC Report
Thu 26 May 2016
SRR1512262_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512262_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2031409
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64760.31879350736360823No Hit
GTCCTACAGTGGACATTTCTAAATT53770.26469312679032136No Hit
GTCCTAAAGTGTGTATTTCTCATTT50070.24647916790759516No Hit
CTGTAGGACGTGGAATATGGCAAGA48480.23865208827961285No Hit
CTTTAGGACGTGAAATATGGCGAGG42950.211429603787322No Hit
GGTATCAACGCAGAGTACTTTTTTT40020.19700611742883878No Hit
TATCAACGCAGAGTACTTTTTTTTT37770.1859300613515053No Hit
GTCCTACAGTGTGCATTTCTCATTT28050.13808149909742448No Hit
CTGAAGGACCTGGAATATGGCGAGA24830.12223043217786277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13150.014.3052621
TTACACG400.005277185714.2510764
CGTGCGC655.494646E-513.14345810
TAGGACC9400.012.1285744
GGACCGT1501.7644197E-1011.4008616
GGCGAGG13150.011.27639319
TACCCGG600.005873391411.084175
CGTATAC600.005873391411.084173
TCCGAGC600.005952805711.0641888
GCGTGCG803.823735E-410.6716739
AAGGCGT1701.382432E-1010.6184486
AGGACCT17050.010.587315
CTATAGT3000.010.45078854
TAGAAAT7300.010.4117454
TCCTATA3200.010.391412
TCGAACT1201.5129626E-610.2975419
GTCCTAT2600.010.2315421
GTATTAT2350.010.1071451
AATGTCC7550.010.0488378
TGTAGGA28850.010.0440862
Produced by FastQC (version 0.11.5)