FastQCFastQC Report
Thu 26 May 2016
SRR1512262_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512262_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2031409
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75320.37077713055322686No Hit
GTCCTACAGTGGACATTTCTAAATT55850.2749323252973675No Hit
CTGTAGGACGTGGAATATGGCAAGA53200.26188719258406357No Hit
GTCCTAAAGTGTGTATTTCTCATTT50110.2466760755711922No Hit
GGTATCAACGCAGAGTACTTTTTTT48270.23761832304572836No Hit
TATCAACGCAGAGTACTTTTTTTTT47250.23259717762400384No Hit
CTTTAGGACGTGAAATATGGCGAGG46380.2283144359407682No Hit
GTCCTACAGTGTGCATTTCTCATTT28500.1402967103128912No Hit
CTGAAGGACCTGGAATATGGCGAGA26510.13050055404893846No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22620.11135128376412629No Hit
CTGTAGGACCTGGAATATGGCGAGA21460.10564096151981212No Hit
TTTCTAAATTTTCCACCTTTTTCAG21040.10357343105204318No Hit
GTCCTTCAGTGTGCATTTCTCATTT20680.10180126207966983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12700.012.6565831
TAGGACC9550.012.3407434
GCCGGTT907.4807413E-611.60871811
CACCTTT8000.011.15525414
GTACTAG1201.2552846E-711.0963531
TATGTCG2100.010.84956116
GTGGTAT4400.010.8081361
TGGTATC4450.010.6785322
CCACCTT7400.010.65294713
TCCAACG1252.2624954E-710.6325718
TTTAGAA8400.010.4090262
GGCGAGG12800.010.383368519
TGTCGAG2200.010.356418
CGGTTTC1201.5286078E-610.28929213
GTCCTAA15550.010.2757221
TGTAGGA28250.010.260842
TAGAAAT7550.010.19673254
GTAGGAC28450.010.1887083
AAATGTC6650.010.1482657
GGCGAAA750.002663559710.12625719
Produced by FastQC (version 0.11.5)