FastQCFastQC Report
Thu 26 May 2016
SRR1512261_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512261_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1440122
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59610.4139232648345071No Hit
GGTATCAACGCAGAGTACTTTTTTT38530.2675467772869243No Hit
TATCAACGCAGAGTACTTTTTTTTT36110.25074264541476343No Hit
GTCCTACAGTGGACATTTCTAAATT28440.19748326877861735No Hit
GTCCTAAAGTGTGTATTTCTCATTT25980.18040138266063568No Hit
CTGTAGGACGTGGAATATGGCAAGA25270.17547124479731577No Hit
CTTTAGGACGTGAAATATGGCGAGG22100.15345922081601418No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17420.1209619740549759No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16950.11769836166658104No Hit
GTCCTACAGTGTGCATTTCTCATTT14980.10401896505990464No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC456.714848E-414.79138615
GCGTTGA456.763006E-414.7774521
AATACGG551.9447388E-413.83090814
GGTATCA10650.013.4691881
ACGGCGA500.001488690813.31271217
TAGGACC3300.012.6681564
GTTCTAA1501.8189894E-1212.6663881
TACGGCG550.003047163812.10204416
TAGTACC1209.991709E-911.8763964
GTATTAA2000.011.8747391
CTTATAC1459.640644E-1111.794493
GTATTAC1552.5465852E-1111.6449041
TCAATAC855.3155887E-511.1777843
TCCAACG1408.3255145E-910.85879818
GTAGTAC1158.7994704E-710.7403933
TGTAGGA13950.010.6249062
GTATTAG909.515099E-510.5553231
GGCGAGG6100.010.44184819
CGCCCTA1553.603418E-910.42894916
GTAGGAC13350.010.3907353
Produced by FastQC (version 0.11.5)