FastQCFastQC Report
Thu 26 May 2016
SRR1512261_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512261_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1440122
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68790.47766786425038993No Hit
GGTATCAACGCAGAGTACTTTTTTT47260.32816664143732266No Hit
TATCAACGCAGAGTACTTTTTTTTT45500.31594545462120566No Hit
GTCCTACAGTGGACATTTCTAAATT26810.18616478326141814No Hit
CTGTAGGACGTGGAATATGGCAAGA26320.18276229375011285No Hit
GTCCTAAAGTGTGTATTTCTCATTT25400.17637394609623352No Hit
CTTTAGGACGTGAAATATGGCGAGG23900.165958161877952No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21370.14839020582978385No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18370.12755863739322085No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14720.1022135624620692No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAA456.7378365E-414.7847279
GGTATCA11750.014.1693851
ACACCGT500.001492975613.3076436
GGCGAAA550.003084643512.08231319
GTCTTAC1302.5556801E-911.7092061
CGAAATC1302.6248017E-911.68927213
TCTAATG1001.920589E-611.4041712
ACGAAAT1451.2205419E-911.13581312
TAGGACC4200.011.0877754
GGCGAGG7450.011.08607219
GCACCGT700.001484155710.8633816
CGCCCTA1408.44193E-910.84904716
CACGTTT1158.897987E-710.73112218
AGCACCG803.7563744E-410.6917835
TGTAGGA15100.010.6363412
GTCCTAC11800.010.5618521
AGGACGT17100.010.3930535
GTCCTAA7450.010.3437861
GTATCAA24850.010.3368491
TTAGGAC10150.010.2993343
Produced by FastQC (version 0.11.5)