FastQCFastQC Report
Thu 26 May 2016
SRR1512260_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512260_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561761
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65170.4172853592835268No Hit
GTCCTACAGTGGACATTTCTAAATT43490.2784677040853242No Hit
CTGTAGGACGTGGAATATGGCAAGA42920.27481797791083273No Hit
GTCCTAAAGTGTGTATTTCTCATTT40930.262075951441994No Hit
GGTATCAACGCAGAGTACTTTTTTT40290.25797801328116143No Hit
TATCAACGCAGAGTACTTTTTTTTT37370.23928117042236294No Hit
CTTTAGGACGTGAAATATGGCGAGG34670.22199299380635065No Hit
GTCCTACAGTGTGCATTTCTCATTT21710.13900974604949157No Hit
CTGAAGGACCTGGAATATGGCGAGA19140.12255396312239837No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18040.11551063190846741No Hit
GTCCTTCAGTGTGCATTTCTCATTT16940.10846730069453649No Hit
ATTTAGAAATGTCCACTGTAGGACG16770.10737878587056533No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16630.10648236189788322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9500.014.3023731
CGAATGG852.6883026E-713.41614819
TTACACC1251.4060788E-912.1624094
TAGGACC7400.012.0699584
ACGTAAA550.0031114612.0684568
CGCCCTA1406.693881E-1011.54654316
GGCGAGG11550.011.51891419
TTCCGAA1001.9077324E-611.41070216
ATTACAC1251.8259016E-811.4022583
TCCAACG1251.8302671E-811.40042518
TGTAGGA25300.011.2670542
CACCGAG855.3119E-511.1786857
GTCTAGG1201.2720557E-711.0851731
GTGGTAT2750.011.0563791
GTAGGAC24950.010.9681033
CCGAATG1053.4640634E-610.85754818
TCCGAAT1158.7087574E-710.74921217
GTATCAA22550.010.7024411
GTGTAGG3150.010.5573071
AGGACGT24850.010.51516155
Produced by FastQC (version 0.11.5)