FastQCFastQC Report
Thu 26 May 2016
SRR1512260_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512260_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561761
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT76430.48938345880067435No Hit
TATCAACGCAGAGTACTTTTTTTTT50170.32123993363901393No Hit
GGTATCAACGCAGAGTACTTTTTTT49050.31406854185755695No Hit
CTGTAGGACGTGGAATATGGCAAGA44670.2860232775693592No Hit
GTCCTACAGTGGACATTTCTAAATT44400.284294459907758No Hit
GTCCTAAAGTGTGTATTTCTCATTT39670.2540081356878549No Hit
CTTTAGGACGTGAAATATGGCGAGG38250.24491583539350772No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22030.14105871512990784No Hit
GTCCTACAGTGTGCATTTCTCATTT21920.14035438200851474No Hit
CTGAAGGACCTGGAATATGGCGAGA21280.1362564438476822No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18890.12095320602832314No Hit
ATTTAGAAATGTCCACTGTAGGACG18050.11557466219223042No Hit
GAGTACTTTTTTTTTTTTTTTTTTT16170.10353696884478483No Hit
TTTCTAAATTTTCCACCTTTTTCAG16070.10289666600715475No Hit
GTCCTTCAGTGTGCATTTCTCATTT16010.1025124843045767No Hit
CTGTAGGACCTGGAATATGGCGAGA15760.10091172721050148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12500.014.4573331
GTATTAG1207.2577677E-1012.681871
TAGGACC7050.012.4009064
GTCCTAT1850.012.3391171
GCGTGCA550.003061526212.094569
TATGTCG1302.6411726E-911.684978516
GGCGAGG11100.011.54681119
GTCTTAC1201.2540295E-711.0966371
CACCTTT6350.011.064280514
TTCCGAA700.001499230610.85033718
CCACCTT6700.010.77384913
AGGACCT15350.010.71007255
TGTAGGA23100.010.5717772
ACCTTTT6950.010.38197815
GTCCTAC22850.010.3647221
GTAGGAC23600.010.348133
GGACCTG15650.010.3249216
GTATCAA26400.010.3040191
CTGTAGG23650.010.2554241
ACCGGGG1303.8731196E-710.22435615
Produced by FastQC (version 0.11.5)