FastQCFastQC Report
Thu 26 May 2016
SRR1512259_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512259_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927545
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57750.622611301877537No Hit
GGTATCAACGCAGAGTACTTTTTTT40380.4353427596504752No Hit
TATCAACGCAGAGTACTTTTTTTTT35330.38089796182395463No Hit
CTGTAGGACGTGGAATATGGCAAGA20060.21626983057425786No Hit
GTCCTACAGTGGACATTTCTAAATT19900.21454484688074432No Hit
GTCCTAAAGTGTGTATTTCTCATTT19620.21152612541709567No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16930.18252483706989958No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15540.16753904123250085No Hit
CTTTAGGACGTGAAATATGGCGAGG15520.16732341827081165No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11970.1290503425709804No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCAC456.757192E-414.77819913
GTATAAC400.00527406314.2511781
GGTATCA9400.013.4425991
TAGGACC2950.013.2044814
ACTGGCG550.003099907412.0736028
CGCGCCT951.0433305E-611.99514612
GATCAAC658.0124545E-411.69327451
TAACAGG658.0124545E-411.69327456
TCCTAGA1157.050585E-811.5661742
GTCCTAA5700.011.167591
TGTAGGA11250.011.1475882
GTATAGT1201.2710552E-711.08424951
TAACACT951.3571835E-511.000914
CTAACAC1053.457213E-610.8580413
TAGGACT1053.457213E-610.8580414
TAGGACA3250.010.8162794
AGGACTG1158.7905573E-710.7400195
CCATTCG803.8063288E-410.6756659
TTATACT1252.2325185E-710.6408814
TCGCGCG909.6119475E-510.5438669
Produced by FastQC (version 0.11.5)