##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512259_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 927545 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.39817582974411 31.0 30.0 34.0 16.0 34.0 2 30.28592682834795 33.0 31.0 34.0 25.0 34.0 3 30.958005271981413 33.0 31.0 34.0 27.0 34.0 4 34.64386094475201 37.0 35.0 37.0 32.0 37.0 5 34.817770566387615 37.0 35.0 37.0 32.0 37.0 6 34.98219385582371 37.0 35.0 37.0 32.0 37.0 7 34.9197602272666 37.0 35.0 37.0 32.0 37.0 8 34.93371318911751 37.0 35.0 37.0 32.0 37.0 9 36.55414454285237 39.0 37.0 39.0 33.0 39.0 10 36.50401651671886 39.0 37.0 39.0 33.0 39.0 11 36.530879903400916 39.0 37.0 39.0 33.0 39.0 12 36.48231083128042 39.0 37.0 39.0 33.0 39.0 13 36.454656108328976 39.0 37.0 39.0 33.0 39.0 14 37.72593243454495 40.0 38.0 41.0 33.0 41.0 15 37.65084712871074 40.0 38.0 41.0 32.0 41.0 16 37.57755257157335 40.0 38.0 41.0 32.0 41.0 17 37.56006231503593 40.0 38.0 41.0 32.0 41.0 18 37.344607539256856 40.0 38.0 41.0 32.0 41.0 19 37.458847818704214 40.0 38.0 41.0 32.0 41.0 20 37.40314378278143 40.0 38.0 41.0 32.0 41.0 21 37.36022726660162 40.0 38.0 41.0 32.0 41.0 22 37.293882237519476 40.0 38.0 41.0 32.0 41.0 23 37.18135616061755 40.0 38.0 41.0 32.0 41.0 24 37.059080691502835 40.0 38.0 41.0 32.0 41.0 25 36.94370300093257 40.0 38.0 41.0 31.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.180821567614256 1101 2 0.36063630828661175 1101 3 -0.14321504912042826 1101 4 -0.23411139950652426 1101 5 -0.4319472622920131 1101 6 -0.4614313733559001 1101 7 -0.44948322177790345 1101 8 -0.5175944042183502 1101 9 -0.6895551778155706 1101 10 -0.7214248568436119 1101 11 -0.7127145580010676 1101 12 -0.8692632252105028 1101 13 -0.7957824521038717 1101 14 -1.2127268566284712 1101 15 -1.0394929573762752 1101 16 -1.2914812636175412 1101 17 -1.3494763288124076 1101 18 -1.3994320527544204 1101 19 -1.3640124694219793 1101 20 -1.5869564638744649 1101 21 -1.6317214772487958 1101 22 -1.6351107300560912 1101 23 -1.7748059202381725 1101 24 -1.7309329869451773 1101 25 -1.948220896773826 1102 1 1.2440889772560375 1102 2 0.4027327156435554 1102 3 -0.020451714694100787 1102 4 -0.06733521564304112 1102 5 -0.2543034017650996 1102 6 -0.20515590734172662 1102 7 -0.37562508552109364 1102 8 -0.4215306220996595 1102 9 -0.35640778421598895 1102 10 -0.6134731245341243 1102 11 -0.5220566579356145 1102 12 -0.6473765944515435 1102 13 -0.5874258763038185 1102 14 -1.0862900149563757 1102 15 -0.881336190585202 1102 16 -0.860923222333696 1102 17 -1.2141679410144093 1102 18 -0.9811708548932074 1102 19 -1.1266401505793198 1102 20 -1.035970874421558 1102 21 -1.211918764967237 1102 22 -1.2152740522025596 1102 23 -1.3481029713733435 1102 24 -1.1701927464950614 1102 25 -1.1494956508173644 1103 1 1.2696260303791718 1103 2 0.36562969138914525 1103 3 -0.21523981974520368 1103 4 -0.2998498009400805 1103 5 -0.3158431902089873 1103 6 -0.3792615375436057 1103 7 -0.48360741682758146 1103 8 -0.5455158119395449 1103 9 -0.6324472369054135 1103 10 -0.8132661272879247 1103 11 -0.5088026735466613 1103 12 -0.790834310951567 1103 13 -0.799505644171191 1103 14 -1.0467991296118981 1103 15 -0.9743370961981483 1103 16 -0.8651124523162608 1103 17 -1.094059021034866 1103 18 -0.9834083965544878 1103 19 -0.9168102456378762 1103 20 -1.04735449083595 1103 21 -1.0805839407608815 1103 22 -1.0343572268996084 1103 23 -1.0820351181688892 1103 24 -0.9528099063203399 1103 25 -1.1137763045836664 1104 1 1.462744089131192 1104 2 0.819617110100566 1104 3 0.09972672063648602 1104 4 0.05780814380139532 1104 5 -0.24512596234131934 1104 6 -0.17692231484535625 1104 7 -0.1410659021159688 1104 8 -0.16873100390451157 1104 9 -0.3472851318410335 1104 10 -0.49547764872686884 1104 11 -0.5026194305011842 1104 12 -0.5011365687381542 1104 13 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24 -0.6161281251321071 1105 25 -0.5143053128385233 1106 1 0.805241415446055 1106 2 -0.03557355090863368 1106 3 -0.5327337888600248 1106 4 -0.7305635877490175 1106 5 -0.6740360963619594 1106 6 -0.834797332534599 1106 7 -0.648425785935359 1106 8 -0.9079370876111597 1106 9 -1.420752895665899 1106 10 -1.375774271183964 1106 11 -1.2240051997685342 1106 12 -1.162168970786162 1106 13 -1.0724009973081294 1106 14 -1.3212945015311632 1106 15 -1.3984535935304763 1106 16 -1.3694646677494546 1106 17 -1.3125419966237217 1106 18 -1.3034849345035724 1106 19 -1.3311916323599462 1106 20 -1.3615157722596507 1106 21 -1.299246522273208 1106 22 -1.578949386455406 1106 23 -1.6292180638234228 1106 24 -1.6837790152753982 1106 25 -1.785138550948993 1107 1 0.49691978204205256 1107 2 -0.15096636681678532 1107 3 -0.28789080928696364 1107 4 -0.22015433830116393 1107 5 -0.28022787328956156 1107 6 -0.1980945536505203 1107 7 -0.03269973994827069 1107 8 -0.10378518833380213 1107 9 -0.1363460519112465 1107 10 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1108 21 -1.3011869298491519 1108 22 -1.3777162450168632 1108 23 -1.1626991301666152 1108 24 -1.0568556907405409 1108 25 -1.2702811053973022 1109 1 0.8299670951916376 1109 2 0.2188229445316452 1109 3 -0.28836235607638727 1109 4 -0.2973797879132931 1109 5 -0.40148497064851796 1109 6 -0.3240246023899047 1109 7 -0.35925748703685656 1109 8 -0.2689657095862614 1109 9 -0.5413274412736158 1109 10 -0.5891648905896218 1109 11 -0.38729156694698474 1109 12 -0.5698899539162596 1109 13 -0.4607247478609864 1109 14 -0.6766998215539104 1109 15 -0.8199274462294497 1109 16 -0.7781544594556138 1109 17 -0.8234200320484177 1109 18 -0.5695492549685994 1109 19 -0.8811168656111406 1109 20 -0.9712544045546707 1109 21 -1.0508524277933091 1109 22 -0.9954528299380598 1109 23 -1.0165730058002111 1109 24 -1.202470702476596 1109 25 -1.3023770252860984 1110 1 0.7645532324695594 1110 2 0.2281208798474843 1110 3 -0.17984206807962266 1110 4 -0.05223620493004688 1110 5 0.04234054567267265 1110 6 -0.0344533243917553 1110 7 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0.7971311817021132 2314 3 0.10603439784049229 2314 4 0.012991729114567363 2314 5 -0.02561715940918674 2314 6 -0.2531748510061007 2314 7 -0.31450657231690116 2314 8 -0.30909318402100894 2314 9 -0.5086115439098862 2314 10 -0.5584132956932919 2314 11 -1.36707391050642 2314 12 -0.909233466440881 2314 13 -0.7770888533117812 2314 14 -0.8172940377092672 2314 15 -0.7567824513894053 2314 16 -0.9185463817747745 2314 17 -1.2369614055356095 2314 18 -1.746119524224632 2314 19 -1.7805211249225223 2314 20 -2.078561023374448 2314 21 -1.5975735085701217 2314 22 -1.265427147416247 2314 23 -0.9790079199572617 2314 24 -0.876226448794263 2314 25 -0.8235533786025471 2315 1 -2.4859591773981826 2315 2 -1.022310326402188 2315 3 -0.3613193724202546 2315 4 -0.13390749569163773 2315 5 -0.022146405357034382 2315 6 -0.07014983338805791 2315 7 9.268314885986229E-4 2315 8 -0.11045417767851973 2315 9 -0.05023856998458598 2315 10 -0.11678626961859351 2315 11 -0.21379695490388428 2315 12 -0.18381944248034898 2315 13 -0.15205361723003108 2315 14 -0.19506447463668053 2315 15 -0.7171506684436721 2315 16 -0.7500914839594159 2315 17 -0.44250227939043896 2315 18 -0.1665828786996144 2315 19 -0.28492563514098634 2315 20 -0.16085048778600708 2315 21 -0.22097026685059973 2315 22 -0.19480963508362237 2315 23 0.022361882720680626 2315 24 0.03805297333399693 2315 25 0.12307717108033245 2316 1 1.4927774989584073 2316 2 0.7526472801869595 2316 3 0.14729860238594483 2316 4 0.0645542291145702 2316 5 -0.07147416698494169 2316 6 -0.23496935858185708 2316 7 -0.24194501928659662 2316 8 -0.28546629008161517 2316 9 -0.5863577560310986 2316 10 -0.5223337502387437 2316 11 -0.45199341808217497 2316 12 -0.479119830077245 2316 13 -0.38719775482692853 2316 14 -0.48696733316381113 2316 15 -0.27761578472274095 2316 16 -0.13710698783538078 2316 17 -0.17188091311136589 2316 18 -0.10786194846705399 2316 19 -0.2598771855285875 2316 20 -0.34089530367747756 2316 21 -0.15166915250951263 2316 22 -0.3681260110526168 2316 23 -0.34702401844211295 2316 24 -0.41576243364274745 2316 25 -0.25366701496618305 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19153.0 3 3.0 4 144.0 5 771.0 6 925.0 7 886.0 8 778.0 9 816.0 10 793.0 11 812.0 12 739.0 13 860.0 14 794.0 15 882.0 16 920.0 17 938.0 18 1129.0 19 1317.0 20 1601.0 21 1875.0 22 2322.0 23 2896.0 24 3401.0 25 4005.0 26 4486.0 27 5106.0 28 6080.0 29 7263.0 30 9454.0 31 12566.0 32 16920.0 33 23363.0 34 33358.0 35 49822.0 36 87787.0 37 150269.0 38 472311.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.119565323200824 19.453052244932394 12.483383790366979 23.9439986414998 2 15.298540138883032 20.216854730683032 41.05525590004227 23.429349230391665 3 18.914982173733915 24.72027847255339 29.78516564856357 26.579573705149123 4 12.740053219613623 16.84754343150813 37.53885777863798 32.87354557024027 5 13.445034736411934 37.57813800723486 35.1718873991044 13.804939857248808 6 31.793036296889472 36.40495773990345 18.020059632070772 13.781946331136306 7 27.768823194701962 31.84360321845745 22.115969182772297 18.271604404068288 8 25.10781548736493 36.201239715894836 20.68283862930505 18.00810616743518 9 26.556373131811835 14.903785877643132 20.689711076621116 37.85012991392392 10 16.007987353357183 28.12127551290543 33.96202765534657 21.90870947839082 11 33.661973200883146 22.236523441932547 25.015318875772834 19.086184481411475 12 23.90751807385154 24.55968963765845 31.461901725796164 20.07089056269384 13 29.334749985408337 22.08993651006392 25.79935277879259 22.77596072573515 14 21.12540123423433 22.73855199022988 27.746279464372563 28.389767311163226 15 23.189457675591832 31.11934857901876 23.82309611012199 21.868097635267418 16 21.868824712189653 27.7622542600693 28.334736582377936 22.03418444536311 17 21.512673806168372 28.58311847719494 28.06161879217296 21.842588924463726 18 21.362433933815634 26.439101467513836 30.81261002036625 21.38585457830428 19 23.276595377195143 26.565799189087656 28.507368593770916 21.650236839946285 20 23.67020816950822 26.48101500998073 29.04921666393025 20.799560156580803 21 22.823468927066255 26.15088340062256 28.12452071276897 22.901126959542218 22 23.43604753478359 27.673129805112566 27.89219057531253 20.99863208479131 23 22.04339772312175 27.470923006758873 28.949683454699116 21.535995815420264 24 23.24503712421468 25.95839318783641 29.424293428408244 21.372276259540662 25 22.42671684768553 26.915522967230483 29.231453456852513 21.426306728231477 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 698.0 1 698.0 2 911.5 3 1125.0 4 1125.0 5 1125.0 6 1924.0 7 2723.0 8 2723.0 9 2723.0 10 3034.0 11 3345.0 12 3345.0 13 3345.0 14 3993.0 15 4641.0 16 4641.0 17 4641.0 18 7974.0 19 11307.0 20 11307.0 21 11307.0 22 17582.5 23 23858.0 24 23858.0 25 23858.0 26 35139.0 27 46420.0 28 46420.0 29 46420.0 30 58606.0 31 70792.0 32 70792.0 33 70792.0 34 79073.0 35 87354.0 36 87354.0 37 87354.0 38 91714.5 39 96075.0 40 96075.0 41 96075.0 42 101957.0 43 107839.0 44 107839.0 45 107839.0 46 113230.0 47 118621.0 48 118621.0 49 118621.0 50 115773.0 51 112925.0 52 112925.0 53 112925.0 54 101443.5 55 89962.0 56 89962.0 57 89962.0 58 77519.5 59 65077.0 60 65077.0 61 65077.0 62 54199.0 63 43321.0 64 43321.0 65 43321.0 66 33470.5 67 23620.0 68 23620.0 69 23620.0 70 17271.5 71 10923.0 72 10923.0 73 10923.0 74 7695.5 75 4468.0 76 4468.0 77 4468.0 78 3042.5 79 1617.0 80 1617.0 81 1617.0 82 1080.0 83 543.0 84 543.0 85 543.0 86 359.0 87 175.0 88 175.0 89 175.0 90 117.5 91 60.0 92 60.0 93 60.0 94 46.5 95 33.0 96 33.0 97 33.0 98 28.0 99 23.0 100 23.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32300319661040705 2 0.2796629813108798 3 0.3003627856330421 4 0.29087537531871765 5 0.27804580909821086 6 0.23761650378148771 7 0.337126500601049 8 0.3037049415392245 9 0.24979920111692697 10 0.2244635031184471 11 0.23891024155162283 12 0.23448997083699444 13 0.25432728331239995 14 0.24527111892145398 15 0.328070336210103 16 0.3772323714752384 17 0.3957759461805088 18 0.10910521861473027 19 0.12732535887746685 20 0.18985601776733205 21 0.18230921410821038 22 0.14209553175317638 23 0.2418211515344269 24 0.3321671724821976 25 0.3691465104118938 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 927545.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.53149386246366 #Duplication Level Percentage of deduplicated Percentage of total 1 85.19766896907949 60.94316534957921 2 10.44132535413439 14.937672009705008 3 2.3562707878907627 5.056427082069316 4 0.8148386373444313 2.331464999444058 5 0.36856056544502863 1.3181843912538604 6 0.19607927962501845 0.8415506272231985 7 0.1277936152828775 0.6398887745088437 8 0.08345263089572615 0.4775593083779265 9 0.055546030112968374 0.35759614608990176 >10 0.29907048753459 3.9919814099075674 >50 0.030155113512232008 1.5155964188885345 >100 0.02545115529601614 3.707980307388983 >500 0.0022724243079221234 1.1392581038924163 >1k 0.0013634545847532743 2.115857935746164 >5k 1.5149495386147492E-4 0.6258171359249218 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5775 0.622611301877537 No Hit GGTATCAACGCAGAGTACTTTTTTT 4038 0.4353427596504752 No Hit TATCAACGCAGAGTACTTTTTTTTT 3533 0.38089796182395463 No Hit CTGTAGGACGTGGAATATGGCAAGA 2006 0.21626983057425786 No Hit GTCCTACAGTGGACATTTCTAAATT 1990 0.21454484688074432 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1962 0.21152612541709567 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1693 0.18252483706989958 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1554 0.16753904123250085 No Hit CTTTAGGACGTGAAATATGGCGAGG 1552 0.16732341827081165 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1197 0.1290503425709804 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 1.0781148084459514E-4 8 0.0 0.0 0.0 0.0 1.0781148084459514E-4 9 0.0 0.0 0.0 0.0 1.0781148084459514E-4 10 0.0 0.0 0.0 0.0 1.0781148084459514E-4 11 0.0 0.0 0.0 0.0 1.0781148084459514E-4 12 0.0 0.0 0.0 0.0 1.0781148084459514E-4 13 0.0 0.0 0.0 0.0 1.0781148084459514E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGCCAC 45 6.757192E-4 14.778199 13 GTATAAC 40 0.005274063 14.251178 1 GGTATCA 940 0.0 13.442599 1 TAGGACC 295 0.0 13.204481 4 ACTGGCG 55 0.0030999074 12.073602 8 CGCGCCT 95 1.0433305E-6 11.995146 12 GATCAAC 65 8.0124545E-4 11.6932745 1 TAACAGG 65 8.0124545E-4 11.6932745 6 TCCTAGA 115 7.050585E-8 11.566174 2 GTCCTAA 570 0.0 11.16759 1 TGTAGGA 1125 0.0 11.147588 2 GTATAGT 120 1.2710552E-7 11.0842495 1 TAACACT 95 1.3571835E-5 11.00091 4 CTAACAC 105 3.457213E-6 10.858041 3 TAGGACT 105 3.457213E-6 10.858041 4 TAGGACA 325 0.0 10.816279 4 AGGACTG 115 8.7905573E-7 10.740019 5 CCATTCG 80 3.8063288E-4 10.675665 9 TTATACT 125 2.2325185E-7 10.640881 4 TCGCGCG 90 9.6119475E-5 10.543866 9 >>END_MODULE