FastQCFastQC Report
Thu 26 May 2016
SRR1512259_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512259_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927545
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65420.7053027076853414No Hit
GGTATCAACGCAGAGTACTTTTTTT47700.5142607636287189No Hit
TATCAACGCAGAGTACTTTTTTTTT42780.46121751505317804No Hit
CTGTAGGACGTGGAATATGGCAAGA21480.2315790608541904No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20550.22155259313564302No Hit
GTCCTACAGTGGACATTTCTAAATT20020.21583858465087946No Hit
GTCCTAAAGTGTGTATTTCTCATTT19400.20915427283851457No Hit
CTTTAGGACGTGAAATATGGCGAGG17210.18554355853354826No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16480.1776733204318928No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13080.14101741694473044No Hit
CTGAAGGACCTGGAATATGGCGAGA10280.11083020230824381No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10000.015.2195791
AGTTCGA400.00528294514.24757210
TAGAACG655.4290107E-513.1587044
GCTCTAT604.045083E-412.6829821
TAGTTCC604.0765357E-412.6713464
CTAATTG550.003058367612.0953753
ATAGGAC1302.59206E-911.6966263
ATACTGT1251.814442E-811.4054416
TTACCCT855.29729E-511.1805984
GATATAG600.005816615711.09760951
AGTCGTC1408.270945E-910.8617398
ACGGTGG1053.4431778E-610.8617388
CTAAAGC700.001485833210.8611533
ATAGCAC1053.445406E-610.8611523
CGCGCCT700.001492537910.85529312
TATACTG1158.7583066E-710.7430965
GGCGAGG5050.010.71689819
GTATCAA23050.010.5645661
AAGTCGT1355.637594E-810.5600247
TAGAGTG1002.3857638E-510.4538595