FastQCFastQC Report
Thu 26 May 2016
SRR1512258_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512258_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1452428
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69990.4818827508144982No Hit
GTCCTACAGTGGACATTTCTAAATT59030.40642290013687427No Hit
GTCCTAAAGTGTGTATTTCTCATTT53910.3711715830319989No Hit
CTGTAGGACGTGGAATATGGCAAGA52710.36290955558554366No Hit
CTTTAGGACGTGAAATATGGCGAGG43900.30225250408281856No Hit
GGTATCAACGCAGAGTACTTTTTTT42380.2917872693173087No Hit
TATCAACGCAGAGTACTTTTTTTTT40750.2805646820358737No Hit
CTGAAGGACCTGGAATATGGCGAGA26550.18279735725282079No Hit
GTCCTACAGTGTGCATTTCTCATTT25020.17226327225859045No Hit
GTCCTTCAGTGTGCATTTCTCATTT22790.1569096712539279No Hit
ATTTAGAAATGTCCACTGTAGGACG20740.14279537436623363No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19700.13563495057930583No Hit
TTTCTAAATTTTCCACCTTTTTCAG19400.13356944371769203No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18560.1277860245051734No Hit
GAATATGGCAAGAAAACTGAAAATC17830.12275995780857984No Hit
CTGTAGGACCTGGAATATGGCGAGA16860.11608148562269524No Hit
GGAATATGGCGAGAAAACTGAAAAT15330.10554740062846488No Hit
ATTCCAGGTCCTTCAGTGTGCATTT15020.10341304353813063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGTA704.3938599E-714.94096615
CCCGTAC759.570067E-713.9463516
GGTATCA11050.013.7556551
GTATAGT957.385279E-813.01
TAGAAAT7100.012.57794
TAGGACC6150.012.5126284
GTCCTAT1850.012.3243251
GATATAC4050.011.9629631
TGTCGAG1750.011.94781218
GGCGAGG12500.011.93942819
TATGTCG1603.6379788E-1211.88609316
GGCCTTA658.022833E-411.6923076
GTCGAGG1552.5465852E-1111.65241119
AATGTCC7500.011.6352348
AAATGTC6950.011.6187057
ATGTCGA1850.011.30785117
CCGTACA1104.9070695E-711.23776117
GAAATGT7450.011.2214776
TTTAGAA8250.011.1708552
GTATTAG1451.2169039E-911.1379311
Produced by FastQC (version 0.11.5)