##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512258_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1452428 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.482083104980074 31.0 30.0 34.0 16.0 34.0 2 30.38346961088605 33.0 31.0 34.0 25.0 34.0 3 31.050255847449925 33.0 31.0 34.0 28.0 34.0 4 34.73306215523248 37.0 35.0 37.0 32.0 37.0 5 34.90959551867631 37.0 35.0 37.0 32.0 37.0 6 35.071614565403586 37.0 35.0 37.0 33.0 37.0 7 35.02459261319666 37.0 35.0 37.0 33.0 37.0 8 35.028315345063575 37.0 35.0 37.0 33.0 37.0 9 36.66802072116484 39.0 37.0 39.0 33.0 39.0 10 36.598942598187314 39.0 37.0 39.0 33.0 39.0 11 36.63278110859884 39.0 37.0 39.0 33.0 39.0 12 36.576040258105735 39.0 37.0 39.0 33.0 39.0 13 36.55859842966398 39.0 37.0 39.0 33.0 39.0 14 37.82812986254741 40.0 38.0 41.0 33.0 41.0 15 37.76922642637019 40.0 38.0 41.0 33.0 41.0 16 37.705356823195366 40.0 38.0 41.0 33.0 41.0 17 37.6818568631285 40.0 38.0 41.0 33.0 41.0 18 37.472437187936336 40.0 38.0 41.0 32.0 41.0 19 37.58241441228068 40.0 38.0 41.0 32.0 41.0 20 37.529001093341634 40.0 38.0 41.0 32.0 41.0 21 37.4789552390893 40.0 38.0 41.0 32.0 41.0 22 37.409314609743134 40.0 38.0 41.0 32.0 41.0 23 37.2986268510384 40.0 38.0 41.0 32.0 41.0 24 37.19040461902414 40.0 38.0 41.0 32.0 41.0 25 37.081525555827895 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9968593508761288 1101 2 0.06837383541317976 1101 3 -0.4309763446509258 1101 4 -0.5337924548999311 1101 5 -0.6859750110012826 1101 6 -0.7661602559857457 1101 7 -0.8936345914906241 1101 8 -0.9520176594204202 1101 9 -0.8638239524680031 1101 10 -1.093543463419273 1101 11 -1.06866760715004 1101 12 -1.323803842264276 1101 13 -1.2863158780712993 1101 14 -1.5529851883486785 1101 15 -1.6841932888756546 1101 16 -1.974452722271046 1101 17 -2.1321621871591248 1101 18 -1.7249058236596326 1101 19 -2.1431473828199685 1101 20 -1.8112233350907232 1101 21 -2.1338173823792204 1101 22 -2.0276656944757363 1101 23 -2.1206828939217104 1101 24 -2.3328325427490526 1101 25 -2.2723917454357334 1102 1 0.8233726767102851 1102 2 0.05320343491460733 1102 3 -0.38780365195066224 1102 4 -0.5530822582683896 1102 5 -0.7392899243973545 1102 6 -0.7565968741007012 1102 7 -0.7550895221360889 1102 8 -0.8173102913811974 1102 9 -1.202133048534023 1102 10 -1.1615039634588271 1102 11 -0.8678510585343346 1102 12 -1.0585634987427355 1102 13 -1.1085795623031558 1102 14 -1.504285549737041 1102 15 -1.4958063389895955 1102 16 -1.5244065116393202 1102 17 -1.5424286652529702 1102 18 -1.4345650998409951 1102 19 -1.531974636094752 1102 20 -1.617590176116508 1102 21 -1.5234328211679795 1102 22 -1.6378409089837547 1102 23 -1.65050852837728 1102 24 -1.853192233186057 1102 25 -1.7007401832462534 1103 1 0.8395043242238636 1103 2 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-0.8474026977387012 2314 12 -0.4601991076971288 2314 13 -0.14874420615382888 2314 14 -0.06543526699908853 2314 15 -0.08195802723017209 2314 16 -0.37989090018979965 2314 17 -0.4127820292151654 2314 18 -1.3630545332301551 2314 19 -1.0375128691796434 2314 20 -1.2239736341052065 2314 21 -0.7266762287862676 2314 22 -0.46557608570905273 2314 23 -0.41166708255406803 2314 24 -0.37578701126253833 2314 25 -0.3846537062382609 2315 1 -2.778780974827189 2315 2 -1.1743881666697185 2315 3 -0.3274003252466855 2315 4 -0.23204962738368096 2315 5 0.02382922211771188 2315 6 0.05215087908865712 2315 7 0.030545935439086236 2315 8 0.0755837614087227 2315 9 0.2045690753182896 2315 10 0.15745148596538172 2315 11 -0.008904753382935837 2315 12 0.015388616527687304 2315 13 -3.141475935848348E-4 2315 14 -0.0879863216685095 2315 15 -0.7386649321592884 2315 16 -0.5293204627174646 2315 17 -0.32716247707815427 2315 18 -0.03973190613067601 2315 19 -0.15826383613886463 2315 20 -0.17213298803882537 2315 21 -0.11701262138169 2315 22 0.010985782944089806 2315 23 -0.07049979311347698 2315 24 0.08313273323503978 2315 25 0.1215355428725502 2316 1 1.9231036136694186 2316 2 1.0227909975511231 2316 3 0.6186274082030643 2316 4 0.4044413036425638 2316 5 0.33864841784176747 2316 6 0.23505536947693173 2316 7 0.18075934908399205 2316 8 0.12198198212591649 2316 9 0.09360577892552158 2316 10 0.11308091610523263 2316 11 0.011854332551820335 2316 12 0.16582827518384846 2316 13 0.17829227752875454 2316 14 0.0857636525212726 2316 15 0.1403335133280521 2316 16 0.22604054113784855 2316 17 0.18946815360562397 2316 18 0.29146070300230065 2316 19 0.31699256995765523 2316 20 0.3489968801373209 2316 21 0.31763708231699184 2316 22 0.16633410292001827 2316 23 0.19346589551793159 2316 24 0.14059513143215696 2316 25 0.2808955084195901 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27931.0 3 0.0 4 201.0 5 1105.0 6 1415.0 7 1303.0 8 1242.0 9 1217.0 10 1105.0 11 1143.0 12 1145.0 13 1157.0 14 1213.0 15 1165.0 16 1277.0 17 1410.0 18 1579.0 19 1866.0 20 2191.0 21 2767.0 22 3459.0 23 4137.0 24 4927.0 25 5849.0 26 6610.0 27 7451.0 28 8904.0 29 10989.0 30 13994.0 31 19001.0 32 25771.0 33 36382.0 34 52569.0 35 78607.0 36 140758.0 37 243824.0 38 736764.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.51686947589897 19.655652289162614 12.290805521270808 24.536672713667603 2 14.132539348481608 19.805413909455368 43.53222055850348 22.529826183559546 3 18.919965300422565 24.989699856936706 29.668897846735256 26.421436995905477 4 11.96907893057044 16.787350019908786 38.84317880849542 32.40039224102535 5 12.501466271612843 38.28052225489804 36.12175643468985 13.096255038799272 6 31.105634293431887 37.33037557587634 18.463980266901743 13.100009863790026 7 27.503938002954282 31.517144555956932 22.66224882930302 18.316668611785765 8 24.173457894388125 38.434000861326446 20.64098423110051 16.75155701318492 9 26.6104960503742 14.631930470581825 20.61179707111672 38.14577640792726 10 15.518476906195017 28.45613911666463 34.85579260785811 21.16959136928224 11 33.78659045932032 21.645010895139713 26.186618326597593 18.38178031894237 12 24.3243597445945 23.934574585124704 32.50329534347791 19.237770326802885 13 29.646881566729355 22.312426959687954 25.869205583556738 22.171485890025952 14 20.26966992820207 23.32287544908197 27.77340882877215 28.63404579394381 15 22.15561090646004 33.19199522828566 23.28419866679185 21.36819519846245 16 20.846947380198113 27.783774749594777 30.039372824946664 21.329905045260446 17 20.7658271214725 29.290949931204445 28.81469959275243 21.12852335457062 18 20.715251104290967 26.473691719316772 32.56391930422605 20.247137872166217 19 23.44701742969252 26.109933077260333 28.311048731997452 22.132000761049696 20 23.60352343900891 27.096404823000302 29.31007366939823 19.989998068592556 21 22.08790731443961 26.46603549692034 27.93301905851317 23.513038130126876 22 23.048701800052942 28.983458153180976 27.940389790876203 20.027450255889878 23 21.21438944213144 28.456918942971367 29.271456018654256 21.057235596242933 24 23.301320064118585 26.281094544535765 29.9501023771095 20.467483014236148 25 22.066971398106837 27.511392899835712 29.670363820058824 20.75127188199863 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 607.0 1 607.0 2 940.0 3 1273.0 4 1273.0 5 1273.0 6 2280.0 7 3287.0 8 3287.0 9 3287.0 10 3807.5 11 4328.0 12 4328.0 13 4328.0 14 5729.5 15 7131.0 16 7131.0 17 7131.0 18 12342.0 19 17553.0 20 17553.0 21 17553.0 22 28588.0 23 39623.0 24 39623.0 25 39623.0 26 59228.5 27 78834.0 28 78834.0 29 78834.0 30 103744.0 31 128654.0 32 128654.0 33 128654.0 34 140082.0 35 151510.0 36 151510.0 37 151510.0 38 156628.5 39 161747.0 40 161747.0 41 161747.0 42 168592.5 43 175438.0 44 175438.0 45 175438.0 46 180959.5 47 186481.0 48 186481.0 49 186481.0 50 177008.0 51 167535.0 52 167535.0 53 167535.0 54 146165.0 55 124795.0 56 124795.0 57 124795.0 58 107198.5 59 89602.0 60 89602.0 61 89602.0 62 74055.5 63 58509.0 64 58509.0 65 58509.0 66 44976.0 67 31443.0 68 31443.0 69 31443.0 70 23117.5 71 14792.0 72 14792.0 73 14792.0 74 10363.0 75 5934.0 76 5934.0 77 5934.0 78 4055.0 79 2176.0 80 2176.0 81 2176.0 82 1468.0 83 760.0 84 760.0 85 760.0 86 504.0 87 248.0 88 248.0 89 248.0 90 176.0 91 104.0 92 104.0 93 104.0 94 69.5 95 35.0 96 35.0 97 35.0 98 32.0 99 29.0 100 29.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2599784636484562 2 0.22018303144803048 3 0.2353989319952521 4 0.22851390912320613 5 0.21853062595873943 6 0.18458746319955274 7 0.30011814699248435 8 0.24097580052160933 9 0.19257408973112608 10 0.17467303026380654 11 0.18582676731652104 12 0.18279735725282079 13 0.198701760087247 14 0.1930560413321693 15 0.26796509018002956 16 0.30810477352405763 17 0.3208420658373427 18 0.10306879239452833 19 0.12393041169682766 20 0.18644641937500517 21 0.1449297314565679 22 0.12420581261170949 23 0.19918371168829024 24 0.2658995833184158 25 0.30135745110945256 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1452428.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.02501271028393 #Duplication Level Percentage of deduplicated Percentage of total 1 84.95714105823697 56.942534592577175 2 10.27270161938936 13.770559132170524 3 2.4202573023880154 4.866533293641425 4 0.8580683023707812 2.300481554907735 5 0.41743901722054655 1.3989427717487781 6 0.23052387040392971 0.927051920630834 7 0.15345544673397293 0.7199747287464783 8 0.1056039689022011 0.5662485886341165 9 0.08496781030817646 0.5125471709283468 >10 0.42688252839731655 5.2840312067465165 >50 0.03485483906587731 1.6231498541347045 >100 0.03151334810696076 4.431258813257472 >500 0.003604392135228596 1.7496234565980113 >1k 0.0025745658108775687 3.28057849318251 >5k 4.1193052974041096E-4 1.6264844220954526 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6999 0.4818827508144982 No Hit GTCCTACAGTGGACATTTCTAAATT 5903 0.40642290013687427 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5391 0.3711715830319989 No Hit CTGTAGGACGTGGAATATGGCAAGA 5271 0.36290955558554366 No Hit CTTTAGGACGTGAAATATGGCGAGG 4390 0.30225250408281856 No Hit GGTATCAACGCAGAGTACTTTTTTT 4238 0.2917872693173087 No Hit TATCAACGCAGAGTACTTTTTTTTT 4075 0.2805646820358737 No Hit CTGAAGGACCTGGAATATGGCGAGA 2655 0.18279735725282079 No Hit GTCCTACAGTGTGCATTTCTCATTT 2502 0.17226327225859045 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2279 0.1569096712539279 No Hit ATTTAGAAATGTCCACTGTAGGACG 2074 0.14279537436623363 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1970 0.13563495057930583 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1940 0.13356944371769203 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1856 0.1277860245051734 No Hit GAATATGGCAAGAAAACTGAAAATC 1783 0.12275995780857984 No Hit CTGTAGGACCTGGAATATGGCGAGA 1686 0.11608148562269524 No Hit GGAATATGGCGAGAAAACTGAAAAT 1533 0.10554740062846488 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 1502 0.10341304353813063 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 6.885022872045981E-5 6.885022872045981E-5 0.0 0.0 0.0 6 6.885022872045981E-5 6.885022872045981E-5 0.0 0.0 6.885022872045981E-5 7 6.885022872045981E-5 6.885022872045981E-5 0.0 0.0 6.885022872045981E-5 8 1.3770045744091963E-4 6.885022872045981E-5 0.0 0.0 6.885022872045981E-5 9 1.3770045744091963E-4 6.885022872045981E-5 0.0 0.0 6.885022872045981E-5 10 1.3770045744091963E-4 6.885022872045981E-5 0.0 6.885022872045981E-5 6.885022872045981E-5 11 1.3770045744091963E-4 6.885022872045981E-5 0.0 6.885022872045981E-5 6.885022872045981E-5 12 1.3770045744091963E-4 6.885022872045981E-5 0.0 6.885022872045981E-5 1.3770045744091963E-4 13 1.3770045744091963E-4 6.885022872045981E-5 0.0 6.885022872045981E-5 2.0655068616137943E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCCGTA 70 4.3938599E-7 14.940966 15 CCCGTAC 75 9.570067E-7 13.94635 16 GGTATCA 1105 0.0 13.755655 1 GTATAGT 95 7.385279E-8 13.0 1 TAGAAAT 710 0.0 12.5779 4 TAGGACC 615 0.0 12.512628 4 GTCCTAT 185 0.0 12.324325 1 GATATAC 405 0.0 11.962963 1 TGTCGAG 175 0.0 11.947812 18 GGCGAGG 1250 0.0 11.939428 19 TATGTCG 160 3.6379788E-12 11.886093 16 GGCCTTA 65 8.022833E-4 11.692307 6 GTCGAGG 155 2.5465852E-11 11.652411 19 AATGTCC 750 0.0 11.635234 8 AAATGTC 695 0.0 11.618705 7 ATGTCGA 185 0.0 11.307851 17 CCGTACA 110 4.9070695E-7 11.237761 17 GAAATGT 745 0.0 11.221477 6 TTTAGAA 825 0.0 11.170855 2 GTATTAG 145 1.2169039E-9 11.137931 1 >>END_MODULE