FastQCFastQC Report
Thu 26 May 2016
SRR1512258_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512258_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1452428
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT79930.5503198781626353No Hit
GTCCTACAGTGGACATTTCTAAATT55880.3847350780899294No Hit
GGTATCAACGCAGAGTACTTTTTTT54840.3775746543030016No Hit
CTGTAGGACGTGGAATATGGCAAGA54670.37640420041475375No Hit
TATCAACGCAGAGTACTTTTTTTTT54010.37186008531920345No Hit
GTCCTAAAGTGTGTATTTCTCATTT50130.34514619657566503No Hit
CTTTAGGACGTGAAATATGGCGAGG45510.3133373909068126No Hit
CTGAAGGACCTGGAATATGGCGAGA29430.20262622312431322No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25870.17811554169982952No Hit
GTCCTACAGTGTGCATTTCTCATTT22870.15746047308369157No Hit
ATTTAGAAATGTCCACTGTAGGACG21560.14844109312131135No Hit
TTTCTAAATTTTCCACCTTTTTCAG21560.14844109312131135No Hit
GTCCTTCAGTGTGCATTTCTCATTT20190.13900861178660837No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19890.13694310492499456No Hit
GAATATGGCAAGAAAACTGAAAATC18440.1269598217605279No Hit
CTGTAGGACCTGGAATATGGCGAGA18420.12682212130308698No Hit
GAGTACTTTTTTTTTTTTTTTTTTT17720.12200260529265479No Hit
GGAATATGGCGAGAAAACTGAAAAT17450.12014364911720236No Hit
ATTCCAGGTCCTTCAGTGTGCATTT15570.10719980611775594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12350.014.5474281
CCAACGA1103.8347935E-812.0858719
GGCGAGG11250.012.07052219
TAGGACC7700.011.7223524
TGTCGAG1552.5465852E-1111.63990718
CGAATGG752.0816798E-411.39524819
CCGAATG752.0823457E-411.39485618
ATAATAC1950.011.2066323
TATGTCG1701.2732926E-1111.17104316
GTCGAGG1451.2241799E-911.13328919
AATACGT600.005869478511.0848215
TTAGTAC951.3578912E-511.0014763
ACCTTTT6600.010.79021315
TAATACT1158.797888E-710.7405714
AGGACCT16150.010.7073185
AAATGTC7550.010.6993797
GTATCAA26550.010.5620651
CACCTTT6850.010.53611614
GTAACAC1106.033497E-610.3650263
CCGGGGG1106.078297E-610.358603516
Produced by FastQC (version 0.11.5)