##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512258_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1452428 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.53091994921607 33.0 31.0 34.0 30.0 34.0 2 31.722433056922615 34.0 31.0 34.0 30.0 34.0 3 31.803691473863076 34.0 31.0 34.0 30.0 34.0 4 35.236269887388566 37.0 35.0 37.0 33.0 37.0 5 35.04210742288086 37.0 35.0 37.0 32.0 37.0 6 35.09581542079883 37.0 35.0 37.0 32.0 37.0 7 35.01365024634612 37.0 35.0 37.0 32.0 37.0 8 34.97351331701124 37.0 35.0 37.0 32.0 37.0 9 36.57771263016136 39.0 37.0 39.0 33.0 39.0 10 36.443409243005505 39.0 37.0 39.0 32.0 39.0 11 36.511927613623534 39.0 37.0 39.0 32.0 39.0 12 36.41105927453891 39.0 37.0 39.0 32.0 39.0 13 36.4037900673906 39.0 37.0 39.0 32.0 39.0 14 37.63884405973997 40.0 38.0 41.0 32.0 41.0 15 37.60554258111245 40.0 38.0 41.0 32.0 41.0 16 37.55119840708111 40.0 38.0 41.0 32.0 41.0 17 37.52410171106588 40.0 38.0 41.0 32.0 41.0 18 37.480277851983026 40.0 38.0 41.0 32.0 41.0 19 37.5126340169702 40.0 38.0 41.0 32.0 41.0 20 37.47013345928335 40.0 38.0 41.0 32.0 41.0 21 37.403666136978906 40.0 38.0 41.0 32.0 41.0 22 37.331310054612004 40.0 38.0 41.0 32.0 41.0 23 37.24671653259232 40.0 38.0 41.0 32.0 41.0 24 37.19437521171445 40.0 38.0 41.0 32.0 41.0 25 37.09871814644168 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4182062459248428 1101 2 0.4137118234411581 1101 3 0.4523721561674634 1101 4 0.4113803175872093 1101 5 0.3463678609682077 1101 6 0.48725891089725337 1101 7 0.5083121361322824 1101 8 0.500846900581017 1101 9 0.5131695195534931 1101 10 0.5219790450492496 1101 11 0.5910485004704285 1101 12 0.4132671289417331 1101 13 0.6001864646740458 1101 14 0.6723809947665629 1101 15 0.665727642313712 1101 16 0.6251942194125419 1101 17 0.6054638486070871 1101 18 0.6349276191428643 1101 19 0.687614458024747 1101 20 0.6191247853167496 1101 21 0.5646954326782136 1101 22 0.6584753636733396 1101 23 0.6813965277837752 1101 24 0.6955199667209797 1101 25 0.728066413960363 1102 1 0.3167155430408748 1102 2 0.2952659670587785 1102 3 0.36453915502329437 1102 4 0.2601712197846737 1102 5 0.2240669381061977 1102 6 0.33727598664217595 1102 7 0.3544278382927004 1102 8 0.39384661695000034 1102 9 0.5768948489610111 1102 10 0.37692975692451824 1102 11 0.4781758971042862 1102 12 0.41550241148260625 1102 13 0.4409315074019986 1102 14 0.59260060621137 1102 15 0.48073461732138156 1102 16 0.5967269266995459 1102 17 0.5213498868220228 1102 18 0.5049175280529283 1102 19 0.5767330601676051 1102 20 0.6088513611571784 1102 21 0.7185565769653621 1102 22 0.6866773552647416 1102 23 0.6517416507773746 1102 24 0.5090769505077049 1102 25 0.538830500485183 1103 1 0.1836730161004354 1103 2 0.19494801210923285 1103 3 0.19685146655204733 1103 4 0.21712429440530912 1103 5 0.20510892083300547 1103 6 0.14189490663903825 1103 7 0.16131959569269583 1103 8 0.1510555838014298 1103 9 -0.009738556064391446 1103 10 0.0941380901025326 1103 11 0.25260121123856294 1103 12 0.3677103905490213 1103 13 0.3480881030102694 1103 14 0.3946074871590426 1103 15 0.21720475825780738 1103 16 0.17156282392517141 1103 17 0.40583029326759856 1103 18 0.2745464936370112 1103 19 0.2934568195655416 1103 20 0.2677347167933277 1103 21 0.31047276806634727 1103 22 0.22795092698352448 1103 23 0.21536711997526226 1103 24 0.37041802708154137 1103 25 0.2962305012306814 1104 1 0.16719433921050353 1104 2 0.3159226018057062 1104 3 0.21081627052065244 1104 4 0.31739224773073715 1104 5 0.20073295334950814 1104 6 0.21421559969669346 1104 7 0.24160782656707624 1104 8 0.17170473694218202 1104 9 0.1998575224024819 1104 10 0.33385764435855947 1104 11 0.2349669856327239 1104 12 0.18827942926646557 1104 13 0.19844567586007855 1104 14 0.31163519679178364 1104 15 0.3789562315484716 1104 16 0.3055797701625096 1104 17 0.3458173482251894 1104 18 0.26350654505507976 1104 19 0.18597675764659982 1104 20 0.3002525728046237 1104 21 0.2955352754214857 1104 22 0.38372640744375275 1104 23 0.4808993603728453 1104 24 0.4382346601031699 1104 25 0.2872377703698845 1105 1 0.27352683449649007 1105 2 0.36702253895215264 1105 3 0.2763657901399412 1105 4 0.3212667202098629 1105 5 0.3237351083284068 1105 6 0.37345282695717685 1105 7 0.45209763531270397 1105 8 0.35381167777005373 1105 9 0.406264095088126 1105 10 0.28137941862451754 1105 11 0.4501820345687051 1105 12 0.3334239924342839 1105 13 0.3815515391984974 1105 14 0.5081670276259445 1105 15 0.4783119826484068 1105 16 0.5849548262817521 1105 17 0.5350334746506178 1105 18 0.37548912564231074 1105 19 0.30001105772741 1105 20 0.40055561671232454 1105 21 0.4062585402145231 1105 22 0.448007632190091 1105 23 0.43836770912026424 1105 24 0.4930945864747258 1105 25 0.39328293513597856 1106 1 0.22866935057966487 1106 2 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0.7265335007451199 1107 15 0.715766554685672 1107 16 0.7241962315200681 1107 17 0.8940835602849404 1107 18 0.6410620401333063 1107 19 0.6244815869892832 1107 20 0.6020959901615015 1107 21 0.6458184633524979 1107 22 0.7001944403274436 1107 23 0.7682321562567367 1107 24 0.7126599400818066 1107 25 0.6561646344550809 1108 1 -0.03859601230159626 1108 2 -0.08613549052522629 1108 3 -0.06043247632679538 1108 4 -0.0791483768899468 1108 5 -0.0011570029980774166 1108 6 0.06696579188421481 1108 7 0.07389937616044762 1108 8 0.005858819634518397 1108 9 -0.006421052847365161 1108 10 0.09567009631167878 1108 11 0.1768341941707945 1108 12 0.03745336009694 1108 13 0.06872741602254706 1108 14 0.15659898461676391 1108 15 0.16659508984220395 1108 16 0.13323409337021985 1108 17 0.1792970887383163 1108 18 0.3970065158335814 1108 19 0.2930622957042246 1108 20 0.30572238700087695 1108 21 0.4114893122585528 1108 22 0.2941912989420956 1108 23 0.333116822238793 1108 24 0.3980236359572231 1108 25 0.262083907962662 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-0.44347834499495065 2314 9 -0.48071620376678226 2314 10 -0.44523253214829595 2314 11 -0.4254345890213216 2314 12 -0.7273692739163025 2314 13 -0.5275518960923975 2314 14 -0.8312442487549347 2314 15 -0.781966931477875 2314 16 -0.8074830834455824 2314 17 -0.7157393374268182 2314 18 -0.8146525993632494 2314 19 -0.6883496906080069 2314 20 -0.5746857265942538 2314 21 -0.6378760471911278 2314 22 -0.46151943762829717 2314 23 -0.3261920533020728 2314 24 -0.5357301058553503 2314 25 -0.42106030297230035 2315 1 -0.615206733561692 2315 2 -0.6269299292662005 2315 3 -0.6492561345076098 2315 4 -0.5293176869415461 2315 5 -0.6533384548236398 2315 6 -0.662259861171421 2315 7 -0.7385132505331029 2315 8 -0.9203982903400743 2315 9 -1.1132716980461765 2315 10 -1.0784703670928621 2315 11 -0.931241754409811 2315 12 -0.7934511608824124 2315 13 -1.0057567247515138 2315 14 -1.1619600268177166 2315 15 -1.1802560755325686 2315 16 -1.2442511014826323 2315 17 -1.182824853512038 2315 18 -1.1590709817154448 2315 19 -1.011196572754109 2315 20 -1.7141638678549995 2315 21 -1.4277817333746086 2315 22 -1.1532116864344601 2315 23 -1.0505808630504134 2315 24 -1.0679460189999475 2315 25 -0.9926007461961817 2316 1 -0.8417225308909977 2316 2 -0.6906301413524609 2316 3 -0.6722567274052267 2316 4 -0.49960083242564934 2316 5 -0.572154842395797 2316 6 -0.736495657438816 2316 7 -0.6892231053174314 2316 8 -0.7051587567528657 2316 9 -0.7696878236814726 2316 10 -0.750702110418338 2316 11 -0.8877528616534889 2316 12 -0.8923450086109597 2316 13 -0.9856203288020993 2316 14 -1.058739796325078 2316 15 -1.1977108634020652 2316 16 -1.1912136073452402 2316 17 -1.0973242974861606 2316 18 -1.3032102920073854 2316 19 -1.2587764590711572 2316 20 -1.3436544773596495 2316 21 -1.55265216607188 2316 22 -1.5402856334512407 2316 23 -1.533160587131789 2316 24 -1.5080365170044487 2316 25 -1.4727354312967549 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16843.0 3 3.0 4 486.0 5 2444.0 6 2880.0 7 2249.0 8 1880.0 9 1776.0 10 1661.0 11 1518.0 12 1382.0 13 1405.0 14 1358.0 15 1440.0 16 1378.0 17 1473.0 18 1637.0 19 1883.0 20 2239.0 21 3010.0 22 3693.0 23 4656.0 24 5614.0 25 6463.0 26 7233.0 27 8611.0 28 9748.0 29 11716.0 30 15164.0 31 19698.0 32 26046.0 33 34801.0 34 46989.0 35 67214.0 36 112679.0 37 263848.0 38 759310.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.046794547964446 20.101779416822136 13.818513130082339 24.03291290513108 2 15.228854875322478 21.038716575772828 41.7306343107089 22.001794238195792 3 19.575428179572413 25.57090609655005 30.45300696488914 24.400658758988396 4 13.080384346586705 17.907714129315895 38.19841840897589 30.81348311512151 5 12.618609808337295 38.69713435672817 35.84251014097505 12.841745693959492 6 31.13783342631046 37.946514046433464 17.93273965715545 12.982912870100622 7 27.39753580331613 31.92813368132689 22.584864759865845 18.089465755491126 8 24.400865005776577 38.58651883399206 20.390051744651068 16.6225644155803 9 26.51271696966816 14.627356726137211 20.53424981210945 38.32567649208517 10 15.515265624763272 28.982374322182018 34.72094628004127 20.781413773013444 11 33.98969489620521 21.57219632861739 26.02279739080612 18.41531138437128 12 24.25175892619743 24.400201689285534 32.36610187237642 18.98193751214062 13 29.746616595214743 22.315937820293815 25.946829848903974 21.99061573558747 14 20.313065944644674 23.45846147066074 27.771037130788905 28.45743545390568 15 22.25939699745025 33.284743706357986 23.327634323235092 21.128224972956673 16 20.933162764373854 28.06917406709199 29.78062426586725 21.217038902666907 17 20.841481081617026 29.503271952099503 28.675024649807483 20.980222316475984 18 20.58921746363006 26.698417135613795 32.54432247496673 20.16804292578942 19 23.5682849714676 26.229082666842928 28.475940247207348 21.72669211448212 20 23.743364476972754 27.244028889914006 29.224048305919126 19.78855832719411 21 22.248462206848497 26.514831252418404 28.061161961600266 23.175544579132833 22 23.150283226530963 28.872581454738494 28.038841315338846 19.938294003391697 23 21.316631471391993 28.474710009067916 29.305557181247515 20.903101338292572 24 23.333787764241247 26.48168574587948 29.906993140847415 20.277533349031856 25 22.03231472256667 27.713038323385454 29.705942496676197 20.54870445737168 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 243.0 1 243.0 2 407.0 3 571.0 4 571.0 5 571.0 6 1606.0 7 2641.0 8 2641.0 9 2641.0 10 3296.0 11 3951.0 12 3951.0 13 3951.0 14 5472.0 15 6993.0 16 6993.0 17 6993.0 18 12513.5 19 18034.0 20 18034.0 21 18034.0 22 29354.0 23 40674.0 24 40674.0 25 40674.0 26 61043.0 27 81412.0 28 81412.0 29 81412.0 30 105990.5 31 130569.0 32 130569.0 33 130569.0 34 142347.0 35 154125.0 36 154125.0 37 154125.0 38 158723.0 39 163321.0 40 163321.0 41 163321.0 42 169707.5 43 176094.0 44 176094.0 45 176094.0 46 180996.5 47 185899.0 48 185899.0 49 185899.0 50 176451.5 51 167004.0 52 167004.0 53 167004.0 54 145623.0 55 124242.0 56 124242.0 57 124242.0 58 105482.0 59 86722.0 60 86722.0 61 86722.0 62 71834.5 63 56947.0 64 56947.0 65 56947.0 66 43718.0 67 30489.0 68 30489.0 69 30489.0 70 22320.0 71 14151.0 72 14151.0 73 14151.0 74 9820.5 75 5490.0 76 5490.0 77 5490.0 78 3745.0 79 2000.0 80 2000.0 81 2000.0 82 1315.0 83 630.0 84 630.0 85 630.0 86 405.0 87 180.0 88 180.0 89 180.0 90 106.0 91 32.0 92 32.0 93 32.0 94 22.5 95 13.0 96 13.0 97 13.0 98 7.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09962628095850534 2 6.88502287204598E-5 3 0.0 4 0.0064030712710027625 5 0.027402391030743004 6 0.03153340475397059 7 0.04661160484375129 8 0.060381650587843255 9 0.05404742954556095 10 0.023546778222397254 11 0.022858275935192657 12 0.04750665781711727 13 0.04378874546621244 14 0.014458548031296559 15 0.008881679504939316 16 0.0023409077764956336 17 0.005783419212518624 18 0.004199863951948048 19 0.003029410063700232 20 0.0012393041169682767 21 0.001514705031850116 22 0.003924463037066209 23 0.0037867625796252895 24 0.004268714180668508 25 0.0017212557180114954 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1452428.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.63713991846808 #Duplication Level Percentage of deduplicated Percentage of total 1 84.14705403407753 55.23171959361641 2 10.815001549381414 14.197315398303939 3 2.5386855353743747 4.998961730830766 4 0.9085893540044098 2.3854882622887192 5 0.43675191791908596 1.4333573373057167 6 0.2540391510793344 1.0004641982497873 7 0.17247390525815495 0.7924485696199887 8 0.11752108694575321 0.6170998421783085 9 0.07986626703092638 0.47179740094871836 >10 0.45467741246248233 5.461276393617269 >50 0.03617460045163844 1.6513405258435758 >100 0.03214905544800456 4.518276349867061 >500 0.0036651428334653936 1.7625495738629902 >1k 0.0027226775334314355 3.0759215372593953 >5k 6.28310200022639E-4 2.4019832862073884 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7993 0.5503198781626353 No Hit GTCCTACAGTGGACATTTCTAAATT 5588 0.3847350780899294 No Hit GGTATCAACGCAGAGTACTTTTTTT 5484 0.3775746543030016 No Hit CTGTAGGACGTGGAATATGGCAAGA 5467 0.37640420041475375 No Hit TATCAACGCAGAGTACTTTTTTTTT 5401 0.37186008531920345 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5013 0.34514619657566503 No Hit CTTTAGGACGTGAAATATGGCGAGG 4551 0.3133373909068126 No Hit CTGAAGGACCTGGAATATGGCGAGA 2943 0.20262622312431322 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2587 0.17811554169982952 No Hit GTCCTACAGTGTGCATTTCTCATTT 2287 0.15746047308369157 No Hit ATTTAGAAATGTCCACTGTAGGACG 2156 0.14844109312131135 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2156 0.14844109312131135 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2019 0.13900861178660837 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1989 0.13694310492499456 No Hit GAATATGGCAAGAAAACTGAAAATC 1844 0.1269598217605279 No Hit CTGTAGGACCTGGAATATGGCGAGA 1842 0.12682212130308698 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1772 0.12200260529265479 No Hit GGAATATGGCGAGAAAACTGAAAAT 1745 0.12014364911720236 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 1557 0.10719980611775594 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.885022872045981E-5 2 0.0 0.0 0.0 0.0 6.885022872045981E-5 3 0.0 0.0 0.0 0.0 6.885022872045981E-5 4 0.0 0.0 0.0 0.0 6.885022872045981E-5 5 0.0 0.0 0.0 0.0 6.885022872045981E-5 6 0.0 0.0 0.0 0.0 6.885022872045981E-5 7 0.0 0.0 0.0 0.0 6.885022872045981E-5 8 0.0 0.0 0.0 0.0 6.885022872045981E-5 9 0.0 0.0 0.0 0.0 6.885022872045981E-5 10 0.0 0.0 0.0 6.885022872045981E-5 6.885022872045981E-5 11 0.0 0.0 0.0 6.885022872045981E-5 6.885022872045981E-5 12 0.0 0.0 0.0 6.885022872045981E-5 6.885022872045981E-5 13 0.0 0.0 0.0 6.885022872045981E-5 6.885022872045981E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1235 0.0 14.547428 1 CCAACGA 110 3.8347935E-8 12.08587 19 GGCGAGG 1125 0.0 12.070522 19 TAGGACC 770 0.0 11.722352 4 TGTCGAG 155 2.5465852E-11 11.639907 18 CGAATGG 75 2.0816798E-4 11.395248 19 CCGAATG 75 2.0823457E-4 11.394856 18 ATAATAC 195 0.0 11.206632 3 TATGTCG 170 1.2732926E-11 11.171043 16 GTCGAGG 145 1.2241799E-9 11.133289 19 AATACGT 60 0.0058694785 11.084821 5 TTAGTAC 95 1.3578912E-5 11.001476 3 ACCTTTT 660 0.0 10.790213 15 TAATACT 115 8.797888E-7 10.740571 4 AGGACCT 1615 0.0 10.707318 5 AAATGTC 755 0.0 10.699379 7 GTATCAA 2655 0.0 10.562065 1 CACCTTT 685 0.0 10.536116 14 GTAACAC 110 6.033497E-6 10.365026 3 CCGGGGG 110 6.078297E-6 10.3586035 16 >>END_MODULE