FastQCFastQC Report
Thu 26 May 2016
SRR1512257_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512257_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1910564
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT76710.40150447721196464No Hit
GGTATCAACGCAGAGTACTTTTTTT47300.24757087435961317No Hit
TATCAACGCAGAGTACTTTTTTTTT43900.2297750821223471No Hit
GTCCTAAAGTGTGTATTTCTCATTT24500.1282343852391231No Hit
GTCCTACAGTGGACATTTCTAAATT24260.12697821166943374No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22460.11755690989676347No Hit
CTGTAGGACGTGGAATATGGCAAGA22190.11614371463086293No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22070.11551562784601825No Hit
CTTTAGGACGTGAAATATGGCGAGG20800.10886837603974533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15850.014.0849781
GGGCGCA751.50297565E-512.6468659
GTATTAG1104.970698E-711.2271171
CGTCGGT1201.2643432E-711.09047219
GCGATAA1201.2889359E-711.07502411
TAGGACC5500.011.0558464
CGCCCTA2200.010.80695916
GGGGCGC1159.0498907E-710.7175588
GCGTCGG1201.5210862E-610.29314818
GTCTTAG2400.010.2915231
TATACTG2350.010.1075735
GTATCAA32700.010.0227981
TGGCGAG12950.09.90490118
AATCCCG1356.3865446E-79.85819819
GGCGAGG6550.09.72381619
GTCTAGA2450.09.6937441
GTGGTAT4850.09.5978051
GTCCTAC15950.09.589211
TGTAGGA16250.09.5303532
GGACCTG9900.09.5011196
Produced by FastQC (version 0.11.5)