##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512257_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1910564 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.924634296469524 31.0 30.0 34.0 16.0 34.0 2 29.800311845088675 31.0 30.0 34.0 22.0 34.0 3 30.450285884168235 33.0 31.0 34.0 26.0 34.0 4 34.10264769984151 37.0 35.0 37.0 30.0 37.0 5 34.26871646278272 37.0 35.0 37.0 31.0 37.0 6 34.41820949206622 37.0 35.0 37.0 31.0 37.0 7 34.35459372206322 37.0 35.0 37.0 31.0 37.0 8 34.35932007511918 37.0 35.0 37.0 31.0 37.0 9 35.917360528095365 39.0 37.0 39.0 31.0 39.0 10 35.8525875081913 39.0 37.0 39.0 31.0 39.0 11 35.87107210226928 39.0 37.0 39.0 31.0 39.0 12 35.805933221813035 39.0 37.0 39.0 31.0 39.0 13 35.77537522951338 39.0 37.0 39.0 31.0 39.0 14 36.9993064875084 40.0 38.0 41.0 31.0 41.0 15 36.918939642953596 40.0 38.0 41.0 31.0 41.0 16 36.84045705875333 40.0 38.0 41.0 31.0 41.0 17 36.79824962681177 40.0 38.0 41.0 30.0 41.0 18 36.58050659386443 40.0 37.0 41.0 30.0 41.0 19 36.683437456164775 40.0 38.0 41.0 30.0 41.0 20 36.605961381037226 40.0 38.0 41.0 30.0 41.0 21 36.554320608992946 40.0 37.0 41.0 30.0 41.0 22 36.47195592505668 40.0 37.0 41.0 30.0 41.0 23 36.343119099909764 40.0 37.0 41.0 30.0 41.0 24 36.20160486641641 40.0 37.0 41.0 29.0 41.0 25 36.0828163830157 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9522258562951649 1101 2 0.13358828321258187 1101 3 -0.33459477961907425 1101 4 -0.42162458334468056 1101 5 -0.6169528759402922 1101 6 -0.6488256801774384 1101 7 -0.8048979937864402 1101 8 -0.6933480210866421 1101 9 -0.8450047977132726 1101 10 -0.9495362246188819 1101 11 -0.917348596443361 1101 12 -1.311299301790946 1101 13 -1.2351628559159593 1101 14 -1.5309801326988293 1101 15 -1.8021083120154415 1101 16 -1.7022255415309147 1101 17 -1.8493323666776078 1101 18 -1.6313247941001734 1101 19 -1.95220599377798 1101 20 -1.8491893596850133 1101 21 -2.068186052038058 1101 22 -1.9837239374888185 1101 23 -1.9883353037166955 1101 24 -2.3320903676244527 1101 25 -2.2138915043948018 1102 1 1.0016346344743852 1102 2 0.27771679754097534 1102 3 -0.1368150911064987 1102 4 -0.26455935904747463 1102 5 -0.46001528496272925 1102 6 -0.5000859886152185 1102 7 -0.47861527186471164 1102 8 -0.4718641143210931 1102 9 -0.5354767727400613 1102 10 -0.6440817356392543 1102 11 -0.6666973700198753 1102 12 -0.9251847904190171 1102 13 -0.7395211666946224 1102 14 -1.1116832172177809 1102 15 -1.1954826743782974 1102 16 -1.202169207798022 1102 17 -1.0223811228880848 1102 18 -1.1036846974715857 1102 19 -0.9576411400974365 1102 20 -1.150474305306723 1102 21 -1.1710192575968819 1102 22 -1.1233674960300135 1102 23 -1.5105713152319282 1102 24 -1.436492728840868 1102 25 -1.445003255555008 1103 1 1.2961894256899562 1103 2 0.41984081091035463 1103 3 -0.10592905025621846 1103 4 -0.05003218737272874 1103 5 -0.37288514826502706 1103 6 -0.3326667166456545 1103 7 -0.37998931683617343 1103 8 -0.5593925052245865 1103 9 -0.632422176573904 1103 10 -0.7002382971151562 1103 11 -0.6736212844373597 1103 12 -0.729426129749271 1103 13 -0.7825902522458748 1103 14 -1.119537931908738 1103 15 -1.1090367737461193 1103 16 -1.0467448631844434 1103 17 -1.0547155585564383 1103 18 -0.9769767917857877 1103 19 -1.1712801913009656 1103 20 -1.3111064013335607 1103 21 -1.2588126982277785 1103 22 -1.4794741737879917 1103 23 -1.6013077592976046 1103 24 -1.69680146420027 1103 25 -1.8986604660629354 1104 1 1.3196571157067822 1104 2 0.6626724014947349 1104 3 0.09799172825384872 1104 4 0.10320982937844292 1104 5 -0.019478064881191415 1104 6 -0.06251728044841798 1104 7 -0.19602187263536308 1104 8 -0.24865850438403214 1104 9 -0.2651531163885963 1104 10 -0.3916704678987841 1104 11 -0.34631188629045084 1104 12 -0.36962232083409674 1104 13 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21 -1.098197775175798 1108 22 -1.1060909321962384 1108 23 -1.1900318613870624 1108 24 -1.3256143014102548 1108 25 -1.2892054430475284 1109 1 0.577395313079073 1109 2 -0.08057499612594654 1109 3 -0.3598370621504401 1109 4 -0.5302637342062226 1109 5 -0.736290579179979 1109 6 -0.7395663988260353 1109 7 -0.696661743374321 1109 8 -0.7914277096982829 1109 9 -0.8298689177743555 1109 10 -1.0103121035166538 1109 11 -0.922440162755457 1109 12 -1.0934969041265816 1109 13 -1.1072736855329879 1109 14 -1.2903477037134223 1109 15 -1.331586200752021 1109 16 -1.318778459766527 1109 17 -1.4040224424207182 1109 18 -1.408911816259156 1109 19 -1.4906246935645413 1109 20 -1.704449677840799 1109 21 -1.6791186987957616 1109 22 -1.8246647507078535 1109 23 -1.731623459419552 1109 24 -1.7327829985047387 1109 25 -2.0094021739361807 1110 1 0.3150288959194931 1110 2 -0.2874978648984303 1110 3 -0.5546164694313731 1110 4 -0.5976049211688448 1110 5 -0.6044570832351042 1110 6 -0.5845255045641906 1110 7 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-0.8889197506679309 2314 16 -1.0987695735106016 2314 17 -1.2820557133273098 2314 18 -2.4145130240255526 2314 19 -2.025324089681341 2314 20 -2.5153124090745536 2314 21 -1.7296862725612456 2314 22 -1.4270606955175182 2314 23 -1.2792456693262935 2314 24 -1.0623861746245993 2314 25 -1.0193648134408377 2315 1 -2.6726179864336608 2315 2 -1.302200196582369 2315 3 -0.7310337870008681 2315 4 -0.6968744401212277 2315 5 -0.5981784161009713 2315 6 -0.4940336858796357 2315 7 -0.4684789194059036 2315 8 -0.5422906360536714 2315 9 -0.4854564478428145 2315 10 -0.5756776666450065 2315 11 -0.6958598336985276 2315 12 -0.7050776957752518 2315 13 -0.582147243788711 2315 14 -0.8188591605690334 2315 15 -1.3405893926350743 2315 16 -1.2298743010667152 2315 17 -1.079637111205031 2315 18 -0.7398399295016134 2315 19 -0.7347447669624358 2315 20 -0.8285771141573335 2315 21 -0.8372302450857774 2315 22 -0.7764902533908895 2315 23 -0.7576748919119964 2315 24 -0.6715549330634261 2315 25 -0.5739423962886079 2316 1 1.387528440970577 2316 2 0.6017993225887999 2316 3 0.0011054880745433593 2316 4 -0.10525363375625574 2316 5 -0.3024679351594486 2316 6 -0.39947494024184493 2316 7 -0.4009682979722058 2316 8 -0.4214951648602039 2316 9 -0.5297189954091834 2316 10 -0.4449057485511929 2316 11 -0.5462326638645294 2316 12 -0.4804457364353425 2316 13 -0.5622882618686305 2316 14 -0.5892889388994291 2316 15 -0.516786863024663 2316 16 -0.45506898039521104 2316 17 -0.3993221683222359 2316 18 -0.18120172689987868 2316 19 -0.44217620189840545 2316 20 -0.5080618760155886 2316 21 -0.4922017522212059 2316 22 -0.6536512596754704 2316 23 -0.5038874257672319 2316 24 -0.6493376359976111 2316 25 -0.6072781087229231 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 56830.0 3 2.0 4 494.0 5 2279.0 6 2705.0 7 2503.0 8 2406.0 9 2324.0 10 2291.0 11 2176.0 12 2321.0 13 2317.0 14 2338.0 15 2286.0 16 2464.0 17 2680.0 18 2970.0 19 3499.0 20 4305.0 21 5171.0 22 6381.0 23 7474.0 24 8822.0 25 10557.0 26 11592.0 27 13054.0 28 15235.0 29 18052.0 30 23022.0 31 30066.0 32 39020.0 33 52788.0 34 73846.0 35 105750.0 36 180041.0 37 305109.0 38 905394.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.68123661609782 19.03525077565573 12.061886480873461 24.221626127372993 2 15.216261477161172 19.862510302540333 41.029256589688536 23.891971630609962 3 18.975605965296598 24.746098939239122 29.268851057662925 27.009444037801355 4 12.528180675538175 16.52582016660069 37.81812530648289 33.12787385137824 5 13.508946541227163 37.594790921908974 35.189413863228104 13.70684867363576 6 32.02210932720188 36.55631592368987 18.05035360441431 13.37122114469394 7 28.116348073550334 31.505710304285184 22.090470885405754 18.287470736758728 8 25.542339613764508 36.04609033445107 20.48508900807274 17.926481043711675 9 26.872136653459634 14.975781270499331 19.99307286610165 38.159009209939384 10 15.68718596326285 28.285958173729963 33.88037000525176 22.146485857755422 11 34.561602626968295 22.202667581781864 24.33198952025191 18.903740270997933 12 23.68118652285335 24.35281907268533 31.331238679318545 20.634755725142774 13 29.33287639816127 21.672792082076192 25.711000399380936 23.2833311203816 14 21.624285143044858 21.686469433141657 27.470500507444797 29.21874491636869 15 23.48015719197401 30.00587211397153 23.538773284927785 22.97519740912667 16 22.772820958569074 27.5589396692799 27.01408077659209 22.654158595558936 17 22.11725105425241 27.860909639937915 27.48141556545931 22.540423740350366 18 22.09300253932921 26.760212075385486 29.22036409977478 21.92642128551052 19 23.689251291973754 26.41469881197635 27.65675450323799 22.239295392811904 20 24.040227862797774 26.413352931953177 27.94077622910458 21.605642976144466 21 23.63468458594531 26.017634701366916 27.252976262269772 23.094704450418003 22 23.781103394050586 27.01501769099725 27.27396147293933 21.92991744201284 23 23.039611031207023 26.710698163511925 27.773740303721826 22.475950501559222 24 23.493439230146972 26.148791326546693 28.10945822433736 22.24831121896898 25 23.20924102676207 26.527699538411092 27.98177816827396 22.28128126655288 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 831.0 1 831.0 2 1290.0 3 1749.0 4 1749.0 5 1749.0 6 3038.0 7 4327.0 8 4327.0 9 4327.0 10 4864.5 11 5402.0 12 5402.0 13 5402.0 14 7096.0 15 8790.0 16 8790.0 17 8790.0 18 15200.0 19 21610.0 20 21610.0 21 21610.0 22 33223.5 23 44837.0 24 44837.0 25 44837.0 26 64377.5 27 83918.0 28 83918.0 29 83918.0 30 107801.0 31 131684.0 32 131684.0 33 131684.0 34 148944.0 35 166204.0 36 166204.0 37 166204.0 38 181066.5 39 195929.0 40 195929.0 41 195929.0 42 210083.0 43 224237.0 44 224237.0 45 224237.0 46 236078.0 47 247919.0 48 247919.0 49 247919.0 50 244571.5 51 241224.0 52 241224.0 53 241224.0 54 218753.5 55 196283.0 56 196283.0 57 196283.0 58 170756.0 59 145229.0 60 145229.0 61 145229.0 62 120892.5 63 96556.0 64 96556.0 65 96556.0 66 74772.0 67 52988.0 68 52988.0 69 52988.0 70 38954.5 71 24921.0 72 24921.0 73 24921.0 74 17509.0 75 10097.0 76 10097.0 77 10097.0 78 6934.0 79 3771.0 80 3771.0 81 3771.0 82 2555.0 83 1339.0 84 1339.0 85 1339.0 86 884.5 87 430.0 88 430.0 89 430.0 90 304.0 91 178.0 92 178.0 93 178.0 94 123.5 95 69.0 96 69.0 97 69.0 98 55.5 99 42.0 100 42.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3502107231163154 2 0.29802717940880286 3 0.3183353187854477 4 0.3082859302279327 5 0.2921650360835858 6 0.24924577245253235 7 0.351990302340042 8 0.3208476659248264 9 0.2624355949342707 10 0.23746914523669452 11 0.25086832998004777 12 0.2491410913217249 13 0.2677219920400468 14 0.2584577119635877 15 0.3480647599347627 16 0.4022895856930205 17 0.4203470807573052 18 0.11441647597254005 19 0.12755395788887472 20 0.18983923071930592 21 0.19062433920036176 22 0.14728635104607854 23 0.2516010978956999 24 0.35717201831501066 25 0.396322761236996 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1910564.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.3016331110805 #Duplication Level Percentage of deduplicated Percentage of total 1 81.81352763742973 55.8799754821497 2 11.847177591437816 16.183631545044 3 3.2634294310419163 6.6869267904878145 4 1.2464263690305875 3.4053182623001366 5 0.5813957422535564 1.9855139339873367 6 0.3302546008184027 1.353415714700693 7 0.20363427782107626 0.9735987612802495 8 0.13211285372611822 0.7218818931567338 9 0.09947706613865698 0.6115001466932314 >10 0.43245463054787847 5.1444158249759795 >50 0.028335019030973845 1.3480068586693628 >100 0.017928882280003403 2.4289725819262027 >500 0.00261521094143473 1.1927300045160991 >1k 0.0011537695329859013 1.6811067882883513 >5k 7.691796886572676E-5 0.40300541182411764 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7671 0.40150447721196464 No Hit GGTATCAACGCAGAGTACTTTTTTT 4730 0.24757087435961317 No Hit TATCAACGCAGAGTACTTTTTTTTT 4390 0.2297750821223471 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2450 0.1282343852391231 No Hit GTCCTACAGTGGACATTTCTAAATT 2426 0.12697821166943374 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2246 0.11755690989676347 No Hit CTGTAGGACGTGGAATATGGCAAGA 2219 0.11614371463086293 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2207 0.11551562784601825 No Hit CTTTAGGACGTGAAATATGGCGAGG 2080 0.10886837603974533 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 5.2340565403723715E-5 0.0 11 0.0 0.0 0.0 1.0468113080744743E-4 0.0 12 0.0 0.0 0.0 1.0468113080744743E-4 0.0 13 0.0 0.0 0.0 1.0468113080744743E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1585 0.0 14.084978 1 GGGCGCA 75 1.50297565E-5 12.646865 9 GTATTAG 110 4.970698E-7 11.227117 1 CGTCGGT 120 1.2643432E-7 11.090472 19 GCGATAA 120 1.2889359E-7 11.075024 11 TAGGACC 550 0.0 11.055846 4 CGCCCTA 220 0.0 10.806959 16 GGGGCGC 115 9.0498907E-7 10.717558 8 GCGTCGG 120 1.5210862E-6 10.293148 18 GTCTTAG 240 0.0 10.291523 1 TATACTG 235 0.0 10.107573 5 GTATCAA 3270 0.0 10.022798 1 TGGCGAG 1295 0.0 9.904901 18 AATCCCG 135 6.3865446E-7 9.858198 19 GGCGAGG 655 0.0 9.723816 19 GTCTAGA 245 0.0 9.693744 1 GTGGTAT 485 0.0 9.597805 1 GTCCTAC 1595 0.0 9.58921 1 TGTAGGA 1625 0.0 9.530353 2 GGACCTG 990 0.0 9.501119 6 >>END_MODULE