FastQCFastQC Report
Thu 26 May 2016
SRR1512257_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512257_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1910564
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT86820.4544207888351293No Hit
GGTATCAACGCAGAGTACTTTTTTT55460.29028077572905175No Hit
TATCAACGCAGAGTACTTTTTTTTT54610.2858318276697352No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26730.1399063313241535No Hit
GTCCTACAGTGGACATTTCTAAATT25440.13315439838707313No Hit
CTGTAGGACGTGGAATATGGCAAGA24400.12771097958508587No Hit
GTCCTAAAGTGTGTATTTCTCATTT23840.12477990792247734No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22460.11755690989676347No Hit
CTTTAGGACGTGAAATATGGCGAGG21850.11436413540713633No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16050.014.6963041
TAGGACC5200.013.705384
GGACCGT751.4739793E-512.6708586
CCAACGT802.8813094E-511.86929119
CCGTATG700.001488536610.8598829
CTAGACC1502.1245796E-910.7693834
CCTATAC1158.795978E-710.7412773
CGACTAA1701.4006218E-1010.61226517
GTATCAA33800.010.5522751
GTCCTAC14250.010.4789141
ACCGTGT1002.3904175E-510.4534588
AAGGCGT1002.3904175E-510.4534586
TAAGACA2200.010.3662514
TCCAACG1201.5338574E-610.28644918
GGCGAGG6150.010.19022119
GTGTAGG2900.010.1670551
TGTAGGA15400.010.1189052
GTAGGAC13950.010.0808683
GGACGTG14350.010.0660486
CCGTGTA856.5791357E-410.0613629
Produced by FastQC (version 0.11.5)