FastQCFastQC Report
Thu 26 May 2016
SRR1512256_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512256_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332150
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT124473.7474032816498566No Hit
GGTATCAACGCAGAGTACTTTTTTT84312.5383110040644286No Hit
TATCAACGCAGAGTACTTTTTTTTT79762.4013247026945654No Hit
GTACTGGTTCACTATCGGTCAGTCA55851.681469215715791No Hit
ACGCAGAGTACTTTTTTTTTTTTTT39021.1747704350444077No Hit
GTACTTTTTTTTTTTTTTTTTTTTT36331.0937829293993677No Hit
ATCATTAACTGAATCCATAGGTTAA31990.9631190727081138No Hit
GGATACCACGTGTCCCGCCCTACTC30080.9056149330121933No Hit
GTACATGGGGGTTAAGCGACTAAGC28820.867680264940539No Hit
GAGTACTTTTTTTTTTTTTTTTTTT27650.8324552160168598No Hit
ATACAGGGTGACAGCCCCGTACACA22980.6918560891163631No Hit
GTATTTAGCCTTGGAGGATGGTCCC21710.6536203522504893No Hit
GGTTAATGAGGCGAACCGGGGGAAC21030.6331476742435647No Hit
GTATCAACGCAGAGTACATGGGGGT19450.585578804756887No Hit
GTTCACTATCGGTCAGTCAGGAGTA19370.5831702544031312No Hit
GTGGATGCCCTGGCAGTCAGAGGCG18920.5696221586632546No Hit
TATTCAGACAGGATACCACGTGTCC18710.5632997139846454No Hit
GGTATCAACGCAGAGTACATGGGGG18590.5596868884540117No Hit
CTATCGGTCAGTCAGGAGTATTTAG18230.5488484118621105No Hit
AGCGTACACGGTGGATGCCCTGGCA17840.5371067288875508No Hit
CCCATATTCAGACAGGATACCACGT17280.52024687641126No Hit
GCAGAGTACTTTTTTTTTTTTTTTT16300.490742134577751No Hit
GCCTTGGAGGATGGTCCCCCCATAT15510.46695769983441215No Hit
GTCAGGAGTATTTAGCCTTGGAGGA15400.4636459430979979No Hit
TCTAAGTACCCCGAGGAAAAGAAAT15000.45160319132921867No Hit
GTGGTATCAACGCAGAGTACATGGG14900.4485925033870239No Hit
ACTTAGATGTTTCAGTTCCCCCGGT14380.43293692608761103No Hit
CGGTACTGGTTCACTATCGGTCAGT13970.42059310552461243No Hit
GGTTCACTATCGGTCAGTCAGGAGT13740.41366852325756437No Hit
ACCCTGTATCGCGCGCCTTTCCAGA13640.4106578353153696No Hit
GCCCAGAGCCTGAATCAGTGTGTGT13300.4004214963119073No Hit
GTTAATGATAGTGTGTCGAAACACA13150.39590546439861507No Hit
TCACGGTACTGGTTCACTATCGGTC12970.3904862261026645No Hit
ACGCAGAGTACATGGGGGTTAAGCG12500.3763359927743489No Hit
GAGTACATGGGGGTTAAGCGACTAA12350.37181996086105673No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG12100.3642932410055698No Hit
GTGGTATCAACGCAGAGTACTTTTT11810.3555622459732049No Hit
GTTATAACCGGCGATTTCCGAATGG11730.35315369561944904No Hit
TATCAACGCAGAGTACATGGGGGTT11340.34141201264488935No Hit
ATCAACGCAGAGTACTTTTTTTTTT10760.32395002258015954No Hit
GTACTTAGATGTTTCAGTTCCCCCG10300.3101008580460635No Hit
GTGCTAATCTGCGATAAGCGTCGGT10270.30919765166340507No Hit
GCCTGAATCAGTGTGTGTGTTAGTG10260.3088965828691856No Hit
CATCTAAGTACCCCGAGGAAAAGAA10200.30709017010386874No Hit
GAATCAGTGTGTGTGTTAGTGGAAG10020.30167093180791815No Hit
GGTGATATGAACCGTTATAACCGGC9620.289628180039139No Hit
GGAGTATTTAGCCTTGGAGGATGGT9560.2878217672738221No Hit
GATATGAACCGTTATAACCGGCGAT9460.2848110793316273No Hit
CTATCATTAACTGAATCCATAGGTT8910.2682522956495559No Hit
GTCCCGCCCTACTCATCGAGCTCAC8660.26072557579406896No Hit
ATCCATAGGTTAATGAGGCGAACCG8650.2604245069998495No Hit
TCATTAACCTATGGATTCAGTTAAT8640.26012343820563No Hit
CACTATCATTAACTGAATCCATAGG8630.2598223694114105No Hit
CCGTTATAACCGGCGATTTCCGAAT7780.23423152190275476No Hit
TCGGTAAGGTGATATGAACCGTTAT7650.23031762757790156No Hit
GCGTACACGGTGGATGCCCTGGCAG7650.23031762757790156No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG7530.2267048020472678No Hit
GGTATCAACGCAGAGTACATGGGGA7490.2255005268703899No Hit
CCCCAGTAGCGGCGAGCGAACGGGG7350.22128556375131717No Hit
GGGTACTTAGATGTTTCAGTTCCCC7160.21556525666114706No Hit
CACACACACTGATTCAGGCTCTGGG6880.20713533042300164No Hit
GCCCTACTCATCGAGCTCACAGCAT6840.20593105524612373No Hit
AGCTCACAGCATGTGCATTTTTGTG6490.19539364744844195No Hit
GTCCCCCCATATTCAGACAGGATAC6440.1938883034773446No Hit
CCTATGGATTCAGTTAATGATAGTG6410.19298509709468614No Hit
AGTCAGGAGTATTTAGCCTTGGAGG6330.1905765467409303No Hit
GTGTTTCGACACACTATCATTAACT6230.1875658587987355No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA6210.18696372121029656No Hit
CCCTGGCAGTCAGAGGCGATGAAGG6170.18575944603341862No Hit
GGGTGACAGCCCCGTACACAAAAAT6140.1848562396507602No Hit
TTTGTGTACGGGGCTGTCACCCTGT6100.1836519644738823No Hit
GGGAAACCCAGTGTGTTTCGACACA5970.17973807014902904No Hit
CATTAACTGAATCCATAGGTTAATG5870.17672738220683426No Hit
ATTTAGCCTTGGAGGATGGTCCCCC5760.17341562547042No Hit
TAAGTACCCCGAGGAAAAGAAATCA5710.1719102814993226No Hit
GGAGGATGGTCCCCCCATATTCAGA5710.1719102814993226No Hit
GATTCAGGCTCTGGGCTGCTCCCCG5680.17100707511666416No Hit
GTACATGGGCATCTAAGTACCCCGA5600.16859852476290835No Hit
CAGTAGCGGCGAGCGAACGGGGAGC5510.16588890561493302No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA5340.16077073611320186No Hit
TTCCAGACGCTTCCACTAACACACA5230.1574589793767876No Hit
GGGGTTAAGCGACTAAGCGTACACG5120.15414722264037334No Hit
TCCCCCAGTAGCGGCGAGCGAACGG4990.15023332831552008No Hit
GGGGAAACCCAGTGTGTTTCGACAC4960.14933012193286166No Hit
GTGCATTTTTGTGTACGGGGCTGTC4940.1487279843444227No Hit
GTGGTATCCTGTCTGAATATGGGGG4840.1457172964022279No Hit
AGGTTAATGAGGCGAACCGGGGGAA4740.1427066084600331No Hit
CACTGATTCAGGCTCTGGGCTGCTC4640.13969592051783833No Hit
GTATCAACGCAGAGTACATGGGGAG4610.1387927141351799No Hit
GGTATCAACGCAGAGTACATGGGCA4550.136986301369863No Hit
GAATATGGGGGGACCATCCTCCAAG4530.13638416378142407No Hit
CCACGTGTCCCGCCCTACTCATCGA4510.1357820261929851No Hit
GGGTTTCCCCATTCGGAAATCGCCG4500.13548095739876562No Hit
TAATGATAGTGTGTCGAAACACACT4440.13367454463344874No Hit
ATGATAGTGTGTCGAAACACACTGG4430.13337347583922926No Hit
AAGTACCCCGAGGAAAAGAAATCAA4380.13186813186813187No Hit
CATTAACCTATGGATTCAGTTAATG4360.1312659942796929No Hit
GTACGGGGCTGTCACCCTGTATCGC4280.1288574439259371No Hit
GGGCTGTCACCCTGTATCGCGCGCC4260.1282553063374981No Hit
ATTCAGACAGGATACCACGTGTCCC4230.12735209995483968No Hit
GTACATGGGGAGCGAACGGGGAGCA4210.1267499623664007No Hit
GTCAGAGGCGATGAAGGACGTGCTA4190.12614782477796177No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT4090.12313713683576696No Hit
CGGTGGATGCCCTGGCAGTCAGAGG4080.1228360680415475No Hit
CCCTCACGGTACTGGTTCACTATCG3920.11801896733403582No Hit
GTACATGGGATAGGTTAATGAGGCG3880.11681469215715791No Hit
GTATCGCGCGCCTTTCCAGACGCTT3870.11651362336293843No Hit
CAACGCAGAGTACTTTTTTTTTTTT3810.11470721059762155No Hit
ATGAAGGACGTGCTAATCTGCGATA3770.11350293542074365No Hit
GAGTAGGGCGGGACACGTGGTATCC3760.11320186662652416No Hit
ACCCAGTGTGTTTCGACACACTATC3750.11290079783230467No Hit
GTCTGAATATGGGGGGACCATCCTC3740.11259972903808521No Hit
GTATCAACGCAGAGTACATGGGGAA3680.11079331627276832No Hit
GGTATCAACGCAGAGTACATGGGAT3650.10989010989010989No Hit
AATCTGCGATAAGCGTCGGTAAGGT3650.10989010989010989No Hit
GACTAAGCGTACACGGTGGATGCCC3640.1095890410958904No Hit
GCATGTGCATTTTTGTGTACGGGGC3620.10898690350745145No Hit
GAGCAGCCCAGAGCCTGAATCAGTG3590.10808369712479302No Hit
TATTTAGCCTTGGAGGATGGTCCCC3580.10778262833057353No Hit
GGATGGTCCCCCCATATTCAGACAG3570.10748155953635406No Hit
GCGATAAGCGTCGGTAAGGTGATAT3560.10718049074213458No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT3550.10687942194791511No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC3550.10687942194791511No Hit
CGTCTGGAAAGGCGCGCGATACAGG3540.10657835315369563No Hit
AACGCAGAGTACTTTTTTTTTTTTT3530.10627728435947613No Hit
ACATGGGGGTTAAGCGACTAAGCGT3520.10597621556525667No Hit
TCAACGCAGAGTACTTTTTTTTTTT3500.10537407797681769No Hit
GATCAACGCAGAGTACTTTTTTTTT3480.10477194038837874No Hit
AACACACACACTGATTCAGGCTCTG3470.10447087159415926No Hit
CACGTGTCCCGCCCTACTCATCGAG3430.10326659641728135No Hit
GCAGAGTACATGGGGGTTAAGCGAC3380.10176125244618396No Hit
GTATCAACGCAGAGTACATGGGCAT3380.10176125244618396No Hit
CAGGGTGACAGCCCCGTACACAAAA3370.10146018365196448No Hit
CCATAGGTTAATGAGGCGAACCGGG3370.10146018365196448No Hit
GTATCAACGCAGAGTACATGGGCGA3360.10115911485774498No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCAA401.283211E-518.9948621
GATCAAC702.4905603E-816.2813111
ATATCAA551.1258449E-515.541251
GGTATCA19350.014.9701111
AAATCGC1058.54925E-1114.48979417
ATCGCCG1058.731149E-1114.4766519
AATCGCC1058.731149E-1114.47227518
CGAATGG1850.014.37883719
CCGAATG1900.013.99621418
CTTTCCA3150.012.6785717
GAAATCG1207.185008E-1012.6785716
GTGGTAT5300.012.1853831
ACAGGGT4250.011.8438553
GGAGATT658.0632884E-411.680329
TGGTATC5600.011.5325952
GCATCTA1201.2797136E-711.0719699
GGCATCT1201.2823875E-711.0702988
AGGGTGA4650.011.0292755
CGGAAAT1303.1888703E-810.9701814
TACAGGG4850.010.9661052
Produced by FastQC (version 0.11.5)