FastQCFastQC Report
Thu 26 May 2016
SRR1512256_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512256_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332150
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT146094.398314014752371No Hit
GGTATCAACGCAGAGTACTTTTTTT99532.996537708866476No Hit
TATCAACGCAGAGTACTTTTTTTTT98932.9784735812133074No Hit
GTACTGGTTCACTATCGGTCAGTCA54511.6411259972903807No Hit
ACGCAGAGTACTTTTTTTTTTTTTT47221.4216468463043805No Hit
GTACTTTTTTTTTTTTTTTTTTTTT38501.1591148577449948No Hit
ATCATTAACTGAATCCATAGGTTAA34791.047418335089568No Hit
GGATACCACGTGTCCCGCCCTACTC33170.9986451904260124No Hit
GAGTACTTTTTTTTTTTTTTTTTTT30560.9200662351347283No Hit
GTACATGGGGGTTAAGCGACTAAGC28440.8562396507601986No Hit
ATACAGGGTGACAGCCCCGTACACA23780.7159415926539214No Hit
GGTTAATGAGGCGAACCGGGGGAAC23660.7123287671232876No Hit
GTATTTAGCCTTGGAGGATGGTCCC21700.6533192834562698No Hit
TATTCAGACAGGATACCACGTGTCC20620.620803853680566No Hit
GCAGAGTACTTTTTTTTTTTTTTTT20430.6150835465903959No Hit
CTATCGGTCAGTCAGGAGTATTTAG19930.600030106879422No Hit
GTATCAACGCAGAGTACATGGGGGT19930.600030106879422No Hit
GTGGATGCCCTGGCAGTCAGAGGCG19830.5970194189372271No Hit
AGCGTACACGGTGGATGCCCTGGCA19240.579256360078278No Hit
GTTCACTATCGGTCAGTCAGGAGTA19060.5738371217823273No Hit
CCCATATTCAGACAGGATACCACGT19050.5735360529881077No Hit
GGTATCAACGCAGAGTACATGGGGG18720.5636007827788649No Hit
TCTAAGTACCCCGAGGAAAAGAAAT16840.5069998494656028No Hit
GTCAGGAGTATTTAGCCTTGGAGGA16680.5021827487580912No Hit
GTGGTATCAACGCAGAGTACATGGG15450.46515128706909525No Hit
CGGTACTGGTTCACTATCGGTCAGT15440.4648502182748758No Hit
ACTTAGATGTTTCAGTTCCCCCGGT15370.46274273671533944No Hit
GCCTTGGAGGATGGTCCCCCCATAT14730.4434743338852928No Hit
GTTAATGATAGTGTGTCGAAACACA14360.43233478849917206No Hit
GGTTCACTATCGGTCAGTCAGGAGT13780.41487279843444225No Hit
GTGGTATCAACGCAGAGTACTTTTT13690.41216317928646695No Hit
TCACGGTACTGGTTCACTATCGGTC13400.4034321842541021No Hit
ACGCAGAGTACATGGGGGTTAAGCG13390.4031311154598826No Hit
ACCCTGTATCGCGCGCCTTTCCAGA13180.3968086707812736No Hit
ATCAACGCAGAGTACTTTTTTTTTT13050.3928947764564203No Hit
TATCAACGCAGAGTACATGGGGGTT12680.38175523107029957No Hit
GCCCAGAGCCTGAATCAGTGTGTGT12630.38024988709920216No Hit
GAGTACATGGGGGTTAAGCGACTAA12060.3630889658286918No Hit
GTTATAACCGGCGATTTCCGAATGG11910.3585729339153997No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG11360.3420141502333283No Hit
CATCTAAGTACCCCGAGGAAAAGAA11190.33689598073159716No Hit
GGAGTATTTAGCCTTGGAGGATGGT11080.3335842239951829No Hit
GATATGAACCGTTATAACCGGCGAT10880.3275628481107933No Hit
GTGCTAATCTGCGATAAGCGTCGGT10870.3272617793165738No Hit
GGTGATATGAACCGTTATAACCGGC10710.3224446786090622No Hit
GTACTTAGATGTTTCAGTTCCCCCG10240.3082944452807467No Hit
CACTATCATTAACTGAATCCATAGG10150.3055848261327713No Hit
GCCTGAATCAGTGTGTGTGTTAGTG9780.2944452807466506No Hit
GAATCAGTGTGTGTGTTAGTGGAAG9660.29083245521601686No Hit
ATCCATAGGTTAATGAGGCGAACCG9310.2802950474183351No Hit
TCATTAACCTATGGATTCAGTTAAT9140.27517687791660395No Hit
CTATCATTAACTGAATCCATAGGTT8780.2643384013247027No Hit
TCGGTAAGGTGATATGAACCGTTAT8220.2474785488484119No Hit
GCGTACACGGTGGATGCCCTGGCAG8180.24627427367153393No Hit
CCGTTATAACCGGCGATTTCCGAAT8080.24326358572933915No Hit
CCCCAGTAGCGGCGAGCGAACGGGG8010.2411561041698028No Hit
GTCCCGCCCTACTCATCGAGCTCAC7610.22911335240102362No Hit
GGGTACTTAGATGTTTCAGTTCCCC7570.22790907722414572No Hit
CACACACACTGATTCAGGCTCTGGG7500.22580159566460933No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG7350.22128556375131717No Hit
GGTATCAACGCAGAGTACATGGGGA7130.21466205027848864No Hit
CCTATGGATTCAGTTAATGATAGTG7050.21225349992473277No Hit
TTTGTGTACGGGGCTGTCACCCTGT7010.21104922474785487No Hit
AGCTCACAGCATGTGCATTTTTGTG6770.20382357368658738No Hit
AGTCAGGAGTATTTAGCCTTGGAGG6740.20292036730392896No Hit
GCCCTACTCATCGAGCTCACAGCAT6640.19990967936173418No Hit
CAGTAGCGGCGAGCGAACGGGGAGC6400.19268402830046666No Hit
CATTAACTGAATCCATAGGTTAATG6390.19238295950624718No Hit
GGGAAACCCAGTGTGTTTCGACACA6370.1917808219178082No Hit
TAAGTACCCCGAGGAAAAGAAATCA6310.18997440915249134No Hit
CCCTGGCAGTCAGAGGCGATGAAGG6160.18545837723919917No Hit
GTGTTTCGACACACTATCATTAACT6080.1830498268854433No Hit
GGAGGATGGTCCCCCCATATTCAGA6050.1821466205027849No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA6000.1806412765316875No Hit
GTACATGGGCATCTAAGTACCCCGA6000.1806412765316875No Hit
GTCCCCCCATATTCAGACAGGATAC5960.17943700135480958No Hit
GGGTGACAGCCCCGTACACAAAAAT5870.17672738220683426No Hit
ATGATAGTGTGTCGAAACACACTGG5750.17311455667620052No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA5660.1704049375282252No Hit
ATTTAGCCTTGGAGGATGGTCCCCC5500.16558783682071354No Hit
GGGGAAACCCAGTGTGTTTCGACAC5380.16197501129007977No Hit
TCCCCCAGTAGCGGCGAGCGAACGG5310.15986752973054344No Hit
GGGGTTAAGCGACTAAGCGTACACG5290.15926539214210447No Hit
CAACGCAGAGTACTTTTTTTTTTTT5200.15655577299412915No Hit
GTGGTATCCTGTCTGAATATGGGGG5180.1559536354056902No Hit
GATTCAGGCTCTGGGCTGCTCCCCG5100.15354508505193437No Hit
GTGCATTTTTGTGTACGGGGCTGTC5080.1529429474634954No Hit
TTCCAGACGCTTCCACTAACACACA5080.1529429474634954No Hit
CGGTGGATGCCCTGGCAGTCAGAGG4840.1457172964022279No Hit
GGGTTTCCCCATTCGGAAATCGCCG4830.14541622760800843No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT4800.14451302122534998No Hit
AAGTACCCCGAGGAAAAGAAATCAA4800.14451302122534998No Hit
TAATGATAGTGTGTCGAAACACACT4760.14330874604847207No Hit
AGGTTAATGAGGCGAACCGGGGGAA4690.14120126448893572No Hit
ATTCAGACAGGATACCACGTGTCCC4640.13969592051783833No Hit
GGTATCAACGCAGAGTACATGGGCA4610.1387927141351799No Hit
GTATCAACGCAGAGTACATGGGGAG4590.13819057654674094No Hit
AACACACACACTGATTCAGGCTCTG4500.13548095739876562No Hit
ACATGGGGGTTAAGCGACTAAGCGT4460.1342766822218877No Hit
CCACGTGTCCCGCCCTACTCATCGA4450.13397561342766823No Hit
CACTGATTCAGGCTCTGGGCTGCTC4430.13337347583922926No Hit
AACGCAGAGTACTTTTTTTTTTTTT4350.13096492548547342No Hit
CATTAACCTATGGATTCAGTTAATG4280.1288574439259371No Hit
GTCAGAGGCGATGAAGGACGTGCTA4250.12795423754327864No Hit
GAATATGGGGGGACCATCCTCCAAG4220.12705103116062022No Hit
GTACGGGGCTGTCACCCTGTATCGC4190.12614782477796177No Hit
GGTATCAACGCAGAGTACATGGGAT4030.12133072407045009No Hit
GGGCTGTCACCCTGTATCGCGCGCC3990.12012644889357219No Hit
GTATCGCGCGCCTTTCCAGACGCTT3990.12012644889357219No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT3860.11621255456871896No Hit
GTACATGGGGAGCGAACGGGGAGCA3840.11561041698027999No Hit
TCAACGCAGAGTACTTTTTTTTTTT3820.11500827939184104No Hit
ATGAAGGACGTGCTAATCTGCGATA3820.11500827939184104No Hit
CCCTCACGGTACTGGTTCACTATCG3810.11470721059762155No Hit
TATTTAGCCTTGGAGGATGGTCCCC3770.11350293542074365No Hit
ACCCAGTGTGTTTCGACACACTATC3770.11350293542074365No Hit
CAGGGTGACAGCCCCGTACACAAAA3760.11320186662652416No Hit
GAGTAGGGCGGGACACGTGGTATCC3730.11229866024386571No Hit
GTACATGGGATAGGTTAATGAGGCG3700.11139545386120728No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC3700.11139545386120728No Hit
AATCTGCGATAAGCGTCGGTAAGGT3700.11139545386120728No Hit
GACTAAGCGTACACGGTGGATGCCC3660.11019117868432937No Hit
GCATGTGCATTTTTGTGTACGGGGC3640.1095890410958904No Hit
GGATGGTCCCCCCATATTCAGACAG3630.10928797230167094No Hit
CACGTGTCCCGCCCTACTCATCGAG3590.10808369712479302No Hit
GTCTGAATATGGGGGGACCATCCTC3570.10748155953635406No Hit
GCGATAAGCGTCGGTAAGGTGATAT3560.10718049074213458No Hit
TTTCAGTTCCCCCGGTTCGCCTCAT3560.10718049074213458No Hit
GAGCAGCCCAGAGCCTGAATCAGTG3530.10627728435947613No Hit
GTATCAACGCAGAGTACATGGGCAT3510.10567514677103718No Hit
AAAATGCACATGCTGTGAGCTCGAT3470.10447087159415926No Hit
CGTGGTATCCTGTCTGAATATGGGG3440.10356766521150082No Hit
CCCAGTGTGTTTCGACACACTATCA3430.10326659641728135No Hit
AGATTCCCCCAGTAGCGGCGAGCGA3430.10326659641728135No Hit
GTATCAACGCAGAGTACATGGGGAA3420.10296552762306187No Hit
CTAACACACACACTGATTCAGGCTC3420.10296552762306187No Hit
CCATAGGTTAATGAGGCGAACCGGG3400.10236339003462291No Hit
CATGGGGGTTAAGCGACTAAGCGTA3370.10146018365196448No Hit
GTATCAACGCAGAGTACATGGGCGA3370.10146018365196448No Hit
CGTCTGGAAAGGCGCGCGATACAGG3360.10115911485774498No Hit
GAGGAAAAGAAATCAACCGAGATTC3360.10115911485774498No Hit
ATCAACGCAGAGTACATGGGGGTTA3330.10025590847508654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18650.015.3380361
AAGCGTG400.005239034514.2616525
CTGAGGC400.00524428114.25950059
TCAAGGC400.00524953314.2573513
AGGGTGA4650.013.6993645
ACAGGGT4700.013.1450743
CAGGGTG4850.012.9345044
GGGTGAC4900.012.8063816
GGTGACA5050.012.4259937
GGGCATA550.003041026912.1007967
GGGGATG550.003041026912.1007967
TACAGGG5350.012.0809952
ACAGCCC5250.011.762650511
GTGACAG5200.011.7018678
TGACAGC5200.011.7001039
CCGTACA5550.011.45538517
GACAGCC5400.011.43591110
CAGCCCC5500.011.40072312
CCCGTAC5600.011.35310416
CCCCGTA5550.011.28610715
Produced by FastQC (version 0.11.5)