FastQCFastQC Report
Thu 26 May 2016
SRR1512255_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512255_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1717427
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69380.403976413553531No Hit
GTCCTACAGTGGACATTTCTAAATT60150.3502332267979949No Hit
GTCCTAAAGTGTGTATTTCTCATTT59290.34522573594103273No Hit
CTGTAGGACGTGGAATATGGCAAGA54860.3194313353638903No Hit
CTTTAGGACGTGAAATATGGCGAGG49930.2907256028931652No Hit
GGTATCAACGCAGAGTACTTTTTTT43450.2529947415523338No Hit
TATCAACGCAGAGTACTTTTTTTTT41730.24297975983840944No Hit
GTCCTACAGTGTGCATTTCTCATTT36140.21043106926815522No Hit
CTGAAGGACCTGGAATATGGCGAGA26550.15459172354923964No Hit
ATTTAGAAATGTCCACTGTAGGACG24830.14457674183531527No Hit
CTGTAGGACCTGGAATATGGCGAGA23180.13496934658649246No Hit
GTCCTTCAGTGTGCATTTCTCATTT21680.12623535090574445No Hit
TTTCTAAATTTTCCACCTTTTTCAG20950.12198480634111376No Hit
GAATATGGCAAGAAAACTGAAAATC20350.11849120806881457No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20290.11814184824158465No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19480.11342549057398073No Hit
GGAATATGGCGAGAAAACTGAAAAT17180.10003336386350047No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATACG350.002166773416.2897011
GGTATCA12150.013.5300621
TAGGACC9100.013.0526454
GTGCGTC604.0839566E-412.6697677
CCCGTAC1001.4272337E-712.36025416
TCCAACG1900.012.00223518
AATACGG802.8459117E-511.8834714
GGACCTA802.8604369E-511.877566
TAGAAAT9200.011.7746214
GCGCAGA658.0076815E-411.695171
CGGTTTC1302.6157068E-911.69243313
GGACCTG17450.011.4895816
AGTACCG752.0693362E-411.4027915
AGGACCT18200.011.38190655
CTCGAAC1354.7148205E-911.26135718
CCCCGTA1104.923113E-711.23527915
GGCGAGG13650.011.21115119
CCGGTTT1451.2278178E-911.13186512
GCCGGTT1451.2332748E-911.12893811
TGCGTCT600.00595277411.0640368
Produced by FastQC (version 0.11.5)