##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512255_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1717427 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.50458214526731 31.0 30.0 34.0 16.0 34.0 2 30.409328606106694 33.0 31.0 34.0 25.0 34.0 3 31.086733817507238 33.0 31.0 34.0 28.0 34.0 4 34.76647042348816 37.0 35.0 37.0 32.0 37.0 5 34.94824991105881 37.0 35.0 37.0 32.0 37.0 6 35.10901948088623 37.0 35.0 37.0 33.0 37.0 7 35.06424843676034 37.0 35.0 37.0 33.0 37.0 8 35.07163914390539 37.0 35.0 37.0 33.0 37.0 9 36.723361749873504 39.0 37.0 39.0 33.0 39.0 10 36.65749228351481 39.0 37.0 39.0 33.0 39.0 11 36.68912041094032 39.0 37.0 39.0 33.0 39.0 12 36.642090173265004 39.0 37.0 39.0 33.0 39.0 13 36.625237055199435 39.0 37.0 39.0 33.0 39.0 14 37.91152229468851 40.0 38.0 41.0 33.0 41.0 15 37.85031852882248 40.0 38.0 41.0 33.0 41.0 16 37.794592142781035 40.0 38.0 41.0 33.0 41.0 17 37.76994946510099 40.0 38.0 41.0 33.0 41.0 18 37.558739323418116 40.0 38.0 41.0 32.0 41.0 19 37.67924051502626 40.0 38.0 41.0 33.0 41.0 20 37.62470777506118 40.0 38.0 41.0 33.0 41.0 21 37.582135951047704 40.0 38.0 41.0 32.0 41.0 22 37.514274551407425 40.0 38.0 41.0 32.0 41.0 23 37.40675731777828 40.0 38.0 41.0 32.0 41.0 24 37.30378642003416 40.0 38.0 41.0 32.0 41.0 25 37.20400168391437 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6615587497432216 1101 2 -0.025748268295426868 1101 3 -0.5417367554241963 1101 4 -0.6788544922834632 1101 5 -0.934807411135445 1101 6 -0.8759883365184535 1101 7 -0.9463882370002636 1101 8 -1.0912910131020297 1101 9 -1.0949746485818395 1101 10 -1.1754832901279357 1101 11 -1.142556427256423 1101 12 -1.3329799700164244 1101 13 -1.3746085899336364 1101 14 -1.6645067922090178 1101 15 -1.696343925318324 1101 16 -1.9216591364652231 1101 17 -1.7468387006376815 1101 18 -1.5554951424450039 1101 19 -1.815411721237183 1101 20 -1.9703792127519009 1101 21 -1.8609082371998298 1101 22 -1.8522816076860877 1101 23 -2.0827105069383762 1101 24 -2.122216900648098 1101 25 -2.142839058664606 1102 1 1.2064745961544716 1102 2 0.37213165896318046 1102 3 -0.08634212028364985 1102 4 -0.19948551877037346 1102 5 -0.3646607576830263 1102 6 -0.47294183627086994 1102 7 -0.465447586966782 1102 8 -0.6102965559612059 1102 9 -0.6805159517172612 1102 10 -0.7542536232157389 1102 11 -0.5988610569217343 1102 12 -0.9534188178753737 1102 13 -0.664353682398243 1102 14 -0.9140139845468838 1102 15 -0.9871153519992291 1102 16 -1.2279673653897447 1102 17 -1.2591703443793705 1102 18 -1.015687699176965 1102 19 -1.0563374070778409 1102 20 -1.2171175204311169 1102 21 -1.2643033929604997 1102 22 -1.2983814285956754 1102 23 -1.3103574712359034 1102 24 -1.3058016610210572 1102 25 -1.4418451541373187 1103 1 1.1448093215016009 1103 2 0.3718074801400988 1103 3 -0.22810801329972108 1103 4 -0.1692986049176426 1103 5 -0.38617738071609153 1103 6 -0.4766289752575332 1103 7 -0.5734476038254854 1103 8 -0.5491988576659779 1103 9 -0.841402775727147 1103 10 -0.7728080315209027 1103 11 -0.8128823002932748 1103 12 -0.8033433400540488 1103 13 -0.8721115661251702 1103 14 -1.0659649931539548 1103 15 -1.1640116225027484 1103 16 -1.1248950071310517 1103 17 -1.0598709640069757 1103 18 -1.1450206494430901 1103 19 -1.2873901558543608 1103 20 -1.2125024502953963 1103 21 -1.3627325832439823 1103 22 -1.311214063112324 1103 23 -1.2139011005073925 1103 24 -1.228065475850677 1103 25 -1.4133427057618277 1104 1 1.1346895892455287 1104 2 0.38608209039433206 1104 3 -0.15914011820134988 1104 4 -0.19334782341763201 1104 5 -0.3011369499906138 1104 6 -0.26515231313896237 1104 7 -0.4016525037160861 1104 8 -0.3643395919399026 1104 9 -0.5160137664037805 1104 10 -0.6304201081163399 1104 11 -0.6256891794751027 1104 12 -0.6816893022704988 1104 13 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-0.1607014456672431 2314 7 -0.2647626593733179 2314 8 -0.2904511138182002 2314 9 -0.4160687661799045 2314 10 -0.35671121122185667 2314 11 -1.3272503502382165 2314 12 -0.8509164737330579 2314 13 -0.6762718288212781 2314 14 -0.6025626985856434 2314 15 -0.6772022804776583 2314 16 -0.7857269049538473 2314 17 -0.9465807924295575 2314 18 -1.7616785333742087 2314 19 -1.4310199083173671 2314 20 -1.9497849924966246 2314 21 -1.3182859590170608 2314 22 -0.9433640117928874 2314 23 -0.6787747482471858 2314 24 -0.872420693615588 2314 25 -0.8405156746987927 2315 1 -2.395346856368974 2315 2 -1.0886577156615083 2315 3 -0.3941469692184256 2315 4 -0.26825319156125005 2315 5 -0.12914099373733023 2315 6 -0.11089889436667733 2315 7 -0.11116064759443134 2315 8 -0.09189565002535005 2315 9 -0.15206383820722635 2315 10 -0.2004736288804594 2315 11 -0.3008252268025089 2315 12 -0.328125176650893 2315 13 -0.33615061748278663 2315 14 -0.3026491595065295 2315 15 -0.89852378802955 2315 16 -0.6527742702711876 2315 17 -0.4123883694873669 2315 18 -0.3810242393752503 2315 19 -0.44190881837241136 2315 20 -0.40981474759934855 2315 21 -0.37159768442326424 2315 22 -0.3697079094714937 2315 23 -0.2667145452473676 2315 24 -0.23511468583848227 2315 25 -0.09694714259951809 2316 1 1.6639523240877487 2316 2 0.9503524209521856 2316 3 0.33676396425542876 2316 4 0.21790248906434329 2316 5 0.05140040362359599 2316 6 -0.08916727381430434 2316 7 0.028423645736829428 2316 8 0.039194819536355396 2316 9 -0.07484210323050178 2316 10 -0.03074316640143593 2316 11 -0.09889264850573198 2316 12 0.011640890218394873 2316 13 -0.08392150952909816 2316 14 -0.042781280939124144 2316 15 -0.053394495235473016 2316 16 0.026186927906522328 2316 17 -0.0032785139629964988 2316 18 0.1542118395859049 2316 19 0.10768609706065035 2316 20 0.14947122032834415 2316 21 0.1597504390986444 2316 22 0.06343698297948208 2316 23 0.05503078933148231 2316 24 -0.018570979274670663 2316 25 0.049467160262125276 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32212.0 3 2.0 4 232.0 5 1269.0 6 1576.0 7 1424.0 8 1327.0 9 1306.0 10 1277.0 11 1396.0 12 1327.0 13 1350.0 14 1346.0 15 1424.0 16 1452.0 17 1612.0 18 1778.0 19 2215.0 20 2638.0 21 3157.0 22 4050.0 23 4755.0 24 5582.0 25 6740.0 26 7553.0 27 8710.0 28 10226.0 29 12746.0 30 16344.0 31 21863.0 32 29793.0 33 41746.0 34 60685.0 35 91162.0 36 163235.0 37 281485.0 38 890432.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.877480899091616 20.285269818579327 12.430189128774343 24.40706015355472 2 13.76772932692476 20.403508915302005 43.83879256702091 21.989969190752323 3 18.43384452991633 25.81390949788772 30.037177933308577 25.715068038887374 4 11.7409051053302 17.34676245217438 39.65081021883323 31.261522223662197 5 12.181161239106451 38.71396300425527 36.47250418814245 12.632371568495829 6 30.115629837481055 38.238367727039595 18.913883574284746 12.7321188611946 7 26.75144316896131 31.862565572702835 23.461783110235736 17.92420814810012 8 23.665492166267217 38.82197707242882 21.172418020942498 16.34011274036146 9 26.484559183959288 14.835613080927148 21.191148665997524 37.48867906911603 10 15.051210170272721 28.96643261581725 35.49461352438573 20.487743689524304 11 33.25401442561577 21.85986694729766 26.75647714349234 18.129641483594234 12 23.875239306130382 24.44823197917621 33.13618962206407 18.540339092629342 13 29.234777990785236 22.770322516639386 26.139458610899563 21.855440881675815 14 19.87976625495576 23.656006843823345 28.398540669046756 28.06568623217414 15 21.76044345711543 33.67409032191754 23.545811521382138 21.0196546995849 16 20.425522716025867 28.14758683305396 30.51988532293676 20.90700512798341 17 20.393557419695636 29.616084012518666 29.278750750863054 20.71160781692264 18 20.240232503374937 27.032422702886706 32.932265788755814 19.79507900498255 19 23.212709569021502 26.40712502608864 28.63121798676155 21.74894741812831 20 23.304733748521215 27.598638233530348 29.602611996891927 19.494016021056513 21 21.84948200716319 26.78597153036949 28.16693582581117 23.19761063665615 22 22.7827598812691 29.456096561269963 28.119878330560965 19.641265226899975 23 20.77414836968415 28.99555036837114 29.59165511707637 20.638646144868332 24 22.828729630241764 26.775044516641838 30.30407976260906 20.09214609050734 25 21.63627589816931 28.111519281371077 29.89132466828377 20.360880152175845 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 803.0 1 803.0 2 1231.5 3 1660.0 4 1660.0 5 1660.0 6 2770.5 7 3881.0 8 3881.0 9 3881.0 10 4680.0 11 5479.0 12 5479.0 13 5479.0 14 7478.5 15 9478.0 16 9478.0 17 9478.0 18 16566.5 19 23655.0 20 23655.0 21 23655.0 22 37569.5 23 51484.0 24 51484.0 25 51484.0 26 76605.5 27 101727.0 28 101727.0 29 101727.0 30 131738.5 31 161750.0 32 161750.0 33 161750.0 34 176451.0 35 191152.0 36 191152.0 37 191152.0 38 197343.5 39 203535.0 40 203535.0 41 203535.0 42 208257.5 43 212980.0 44 212980.0 45 212980.0 46 215341.5 47 217703.0 48 217703.0 49 217703.0 50 204161.0 51 190619.0 52 190619.0 53 190619.0 54 163219.5 55 135820.0 56 135820.0 57 135820.0 58 114504.5 59 93189.0 60 93189.0 61 93189.0 62 75938.0 63 58687.0 64 58687.0 65 58687.0 66 44842.5 67 30998.0 68 30998.0 69 30998.0 70 22545.0 71 14092.0 72 14092.0 73 14092.0 74 9850.0 75 5608.0 76 5608.0 77 5608.0 78 3827.0 79 2046.0 80 2046.0 81 2046.0 82 1369.5 83 693.0 84 693.0 85 693.0 86 453.0 87 213.0 88 213.0 89 213.0 90 149.0 91 85.0 92 85.0 93 85.0 94 66.5 95 48.0 96 48.0 97 48.0 98 45.0 99 42.0 100 42.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2878142709995825 2 0.2506656760374677 3 0.26685268136578727 4 0.2581186856850393 5 0.24787079741962834 6 0.20938298978646544 7 0.3141909379554415 8 0.27028805300021486 9 0.22050427761995126 10 0.19866928841808124 11 0.2136917609889678 12 0.21066397581964183 13 0.22545354183904176 14 0.21922329158677484 15 0.29480146754418096 16 0.33992711189471225 17 0.3530281054158343 18 0.10707878704597051 19 0.12349869892577675 20 0.1853353883454726 21 0.16466493190103568 22 0.13322254745034287 23 0.22137767718802603 24 0.29963427848752816 25 0.3332310485394721 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1717427.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.86755011064675 #Duplication Level Percentage of deduplicated Percentage of total 1 84.80503842645484 57.55510195042747 2 10.397445105943136 14.11298253500589 3 2.4564938555409475 5.001486595122633 4 0.8876663957471113 2.4097497437961706 5 0.4082809561995169 1.3854514127046742 6 0.2432426538914274 0.9904969801213892 7 0.14933844336142013 0.7094664001794013 8 0.09480659564933074 0.5147433104840622 9 0.07757202988135349 0.4738161262641611 >10 0.4123985894140676 5.156773474810995 >50 0.03373157706413299 1.5871626994631511 >100 0.027791106481657973 3.867866293618241 >500 0.0034412579839978403 1.621185580411107 >1k 0.0024088805887984882 3.190931828677066 >5k 3.4412579839978403E-4 1.4227850689137023 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6938 0.403976413553531 No Hit GTCCTACAGTGGACATTTCTAAATT 6015 0.3502332267979949 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5929 0.34522573594103273 No Hit CTGTAGGACGTGGAATATGGCAAGA 5486 0.3194313353638903 No Hit CTTTAGGACGTGAAATATGGCGAGG 4993 0.2907256028931652 No Hit GGTATCAACGCAGAGTACTTTTTTT 4345 0.2529947415523338 No Hit TATCAACGCAGAGTACTTTTTTTTT 4173 0.24297975983840944 No Hit GTCCTACAGTGTGCATTTCTCATTT 3614 0.21043106926815522 No Hit CTGAAGGACCTGGAATATGGCGAGA 2655 0.15459172354923964 No Hit ATTTAGAAATGTCCACTGTAGGACG 2483 0.14457674183531527 No Hit CTGTAGGACCTGGAATATGGCGAGA 2318 0.13496934658649246 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2168 0.12623535090574445 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2095 0.12198480634111376 No Hit GAATATGGCAAGAAAACTGAAAATC 2035 0.11849120806881457 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2029 0.11814184824158465 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1948 0.11342549057398073 No Hit GGAATATGGCGAGAAAACTGAAAAT 1718 0.10003336386350047 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.822663787165335E-5 7 0.0 0.0 0.0 0.0 5.822663787165335E-5 8 0.0 0.0 0.0 0.0 5.822663787165335E-5 9 0.0 0.0 0.0 0.0 5.822663787165335E-5 10 0.0 0.0 0.0 0.0 5.822663787165335E-5 11 0.0 0.0 0.0 0.0 5.822663787165335E-5 12 0.0 0.0 0.0 0.0 1.7467991361496007E-4 13 0.0 0.0 0.0 5.822663787165335E-5 1.7467991361496007E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCATACG 35 0.0021667734 16.289701 1 GGTATCA 1215 0.0 13.530062 1 TAGGACC 910 0.0 13.052645 4 GTGCGTC 60 4.0839566E-4 12.669767 7 CCCGTAC 100 1.4272337E-7 12.360254 16 TCCAACG 190 0.0 12.002235 18 AATACGG 80 2.8459117E-5 11.88347 14 GGACCTA 80 2.8604369E-5 11.87756 6 TAGAAAT 920 0.0 11.774621 4 GCGCAGA 65 8.0076815E-4 11.69517 1 CGGTTTC 130 2.6157068E-9 11.692433 13 GGACCTG 1745 0.0 11.489581 6 AGTACCG 75 2.0693362E-4 11.402791 5 AGGACCT 1820 0.0 11.3819065 5 CTCGAAC 135 4.7148205E-9 11.261357 18 CCCCGTA 110 4.923113E-7 11.235279 15 GGCGAGG 1365 0.0 11.211151 19 CCGGTTT 145 1.2278178E-9 11.131865 12 GCCGGTT 145 1.2332748E-9 11.128938 11 TGCGTCT 60 0.005952774 11.064036 8 >>END_MODULE