FastQCFastQC Report
Thu 26 May 2016
SRR1512255_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512255_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1717427
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT81270.47320788598292673No Hit
GTCCTACAGTGGACATTTCTAAATT58090.33823853939643433No Hit
CTGTAGGACGTGGAATATGGCAAGA56790.3306690764731194No Hit
GTCCTAAAGTGTGTATTTCTCATTT55640.32397301311787924No Hit
GGTATCAACGCAGAGTACTTTTTTT54090.314947884247773No Hit
TATCAACGCAGAGTACTTTTTTTTT52900.3080189143410462No Hit
CTTTAGGACGTGAAATATGGCGAGG51420.29940137193604155No Hit
GTCCTACAGTGTGCATTTCTCATTT33630.1958161831623702No Hit
CTGAAGGACCTGGAATATGGCGAGA28780.16757626379461835No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25690.14958423269227744No Hit
CTGTAGGACCTGGAATATGGCGAGA25620.14917664622717589No Hit
TTTCTAAATTTTCCACCTTTTTCAG24480.1425388095098074No Hit
ATTTAGAAATGTCCACTGTAGGACG23460.13659969244689876No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21150.12314933909854683No Hit
GAATATGGCAAGAAAACTGAAAATC20890.12163544651388385No Hit
GTCCTTCAGTGTGCATTTCTCATTT20050.11674440893266497No Hit
GGAATATGGCGAGAAAACTGAAAAT17990.10474972153110439No Hit
GAGTACTTTTTTTTTTTTTTTTTTT17530.10207129618900831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14350.014.8465251
CACTGCG400.005263199614.2566027
AGACGTG400.005263199614.2566026
GCGCCAC655.4577664E-513.15265613
TAGGACC10300.012.6391964
CTGCGTC550.00306271812.0940439
TTCGATT550.003070079312.09016912
GGCGAGG14750.011.90827919
CACCTTT7900.011.53754514
GTCTAGG1001.9029612E-611.4132661
CCACCTT8200.011.23678913
CGGGGGA855.356816E-511.16960217
TAACGTG600.00586094311.0871755
TAGAAAT7950.010.8770334
CTGCGGC700.00148831810.8599579
TTCCACC8100.010.67218611
AGGACCT19800.010.6552085
GGACCTG18700.010.5717416
AAATGTC7850.010.533547
ACCTTTT8850.010.5133115
Produced by FastQC (version 0.11.5)