##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512255_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1717427 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.692480087945516 33.0 31.0 34.0 30.0 34.0 2 31.879467366007404 34.0 31.0 34.0 30.0 34.0 3 31.965337100208625 34.0 31.0 34.0 30.0 34.0 4 35.37163035168307 37.0 35.0 37.0 33.0 37.0 5 35.20098437953986 37.0 35.0 37.0 33.0 37.0 6 35.249027760714135 37.0 35.0 37.0 33.0 37.0 7 35.16968930848298 37.0 35.0 37.0 33.0 37.0 8 35.13290171867567 37.0 35.0 37.0 33.0 37.0 9 36.75556923234583 39.0 37.0 39.0 33.0 39.0 10 36.643266933616395 39.0 37.0 39.0 33.0 39.0 11 36.71156852663898 39.0 37.0 39.0 33.0 39.0 12 36.61690831691828 39.0 37.0 39.0 33.0 39.0 13 36.60304571897379 39.0 37.0 39.0 33.0 39.0 14 37.87936430485837 40.0 38.0 41.0 33.0 41.0 15 37.857218967676644 40.0 38.0 41.0 33.0 41.0 16 37.808996248457724 40.0 38.0 41.0 33.0 41.0 17 37.78662499192105 40.0 38.0 41.0 33.0 41.0 18 37.743381232506536 40.0 38.0 41.0 33.0 41.0 19 37.77911492016837 40.0 38.0 41.0 33.0 41.0 20 37.736580943469505 40.0 38.0 41.0 33.0 41.0 21 37.671211643930135 40.0 38.0 41.0 33.0 41.0 22 37.60571773938572 40.0 38.0 41.0 32.0 41.0 23 37.53061702185886 40.0 38.0 41.0 32.0 41.0 24 37.4806771990891 40.0 38.0 41.0 32.0 41.0 25 37.4019501265556 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3704201599537349 1101 2 0.33609550690918155 1101 3 0.39328187749771715 1101 4 0.375097102408283 1101 5 0.20550335061252412 1101 6 0.33801632452229313 1101 7 0.4582108154271225 1101 8 0.39721531938106125 1101 9 0.4754607168999314 1101 10 0.5157880057767343 1101 11 0.42352509952392836 1101 12 0.41917492925792743 1101 13 0.47513247369828093 1101 14 0.7000762957984605 1101 15 0.5437752961363955 1101 16 0.515840040889195 1101 17 0.5976888534606886 1101 18 0.6217835019052558 1101 19 0.5696544893395199 1101 20 0.5417094882470366 1101 21 0.5456852817665805 1101 22 0.6287628313593032 1101 23 0.5339812944676652 1101 24 0.5824607505890143 1101 25 0.6817770127596035 1102 1 0.43133416819785353 1102 2 0.40589932183309685 1102 3 0.4426237220489817 1102 4 0.42330608921317747 1102 5 0.41516965202103506 1102 6 0.39724158943140253 1102 7 0.47198725271312014 1102 8 0.43842756442444397 1102 9 0.5096649497741836 1102 10 0.5121876558851142 1102 11 0.49454539134750775 1102 12 0.5415315642196035 1102 13 0.6151309321973741 1102 14 0.7243894735226064 1102 15 0.7557553031604058 1102 16 0.790975776588688 1102 17 0.7649289778569042 1102 18 0.742341234386501 1102 19 0.7155640948534412 1102 20 0.6647114449352216 1102 21 0.7449719013205183 1102 22 0.7665904639265619 1102 23 0.733376255359353 1102 24 0.7062690867641876 1102 25 0.7442190701397919 1103 1 0.3564548308572988 1103 2 0.3657425920844375 1103 3 0.38367796703544954 1103 4 0.3960582093707785 1103 5 0.43845187304285815 1103 6 0.3256823630492178 1103 7 0.32195918999592976 1103 8 0.5240441399019176 1103 9 0.43068157175357413 1103 10 0.46980030890419044 1103 11 0.46076125159446946 1103 12 0.45752394127574547 1103 13 0.5044974453327598 1103 14 0.5943354624506512 1103 15 0.7114446590666077 1103 16 0.7915886607124918 1103 17 0.7353942851032613 1103 18 0.8126652095002456 1103 19 0.5884550885827053 1103 20 0.6507570041770592 1103 21 0.6531035565572196 1103 22 0.613003458615367 1103 23 0.6896331876231869 1103 24 0.7640231421604824 1103 25 0.6773163582065678 1104 1 -0.027069330501397815 1104 2 -0.03173683102621894 1104 3 0.08408053360655643 1104 4 0.04983656410336579 1104 5 -0.009771491484421801 1104 6 0.020316906514757704 1104 7 0.0011284286342885252 1104 8 -0.04839943182027184 1104 9 -0.025665429517125915 1104 10 -0.07196270906636215 1104 11 -0.06179064421088043 1104 12 -0.04504791883220349 1104 13 -0.06113637106260228 1104 14 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-0.375213238311197 2314 6 -0.5542583124859988 2314 7 -0.6823366661722545 2314 8 -0.6342211671993496 2314 9 -0.7367296419070186 2314 10 -0.704714863980584 2314 11 -0.6779466620576713 2314 12 -0.7735598874853551 2314 13 -0.8602462096694197 2314 14 -1.0180135691328474 2314 15 -1.0127878623871425 2314 16 -0.9436955641845373 2314 17 -0.9903847817029785 2314 18 -0.9897517085548202 2314 19 -0.9584436362136017 2314 20 -1.006286197908274 2314 21 -0.8492464311737749 2314 22 -0.8948049244773486 2314 23 -0.8097140637931659 2314 24 -0.881190751745514 2314 25 -1.0001608605738994 2315 1 -0.4551092011254525 2315 2 -0.562575639019105 2315 3 -0.545701830588122 2315 4 -0.40469698292714895 2315 5 -0.3455591488756866 2315 6 -0.7694212379324199 2315 7 -0.7197542498189904 2315 8 -0.8208261588703678 2315 9 -0.9605942398153076 2315 10 -1.0050677416947664 2315 11 -0.9505317236677087 2315 12 -0.9743639348435238 2315 13 -0.9847183402634698 2315 14 -1.028381499672605 2315 15 -1.1172835765868712 2315 16 -1.2236721235657058 2315 17 -1.2697234352234616 2315 18 -1.1637616341486137 2315 19 -1.1408316688598106 2315 20 -2.0380419634495013 2315 21 -1.5361757954380835 2315 22 -1.3060074369869668 2315 23 -1.1866389378090645 2315 24 -1.1965498689908145 2315 25 -1.1666231559787406 2316 1 -0.703309162818762 2316 2 -0.6133715444081389 2316 3 -0.5937697827213491 2316 4 -0.5121838962546903 2316 5 -0.6376198848463446 2316 6 -0.6329915564585278 2316 7 -0.7481744961969738 2316 8 -0.6112659972107366 2316 9 -0.7117604624959881 2316 10 -0.7632593650285173 2316 11 -0.7781531617579489 2316 12 -0.8585442555820038 2316 13 -0.8555520778487491 2316 14 -0.9912546788751939 2316 15 -1.1754698184671923 2316 16 -1.000172261573944 2316 17 -1.129586723160351 2316 18 -1.2181692856109692 2316 19 -1.2324361578792349 2316 20 -1.3970501017887287 2316 21 -1.3734649471030664 2316 22 -1.3885242135233682 2316 23 -1.337104568549158 2316 24 -1.3850621825856564 2316 25 -1.3076859023755318 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17869.0 3 0.0 4 476.0 5 2597.0 6 3112.0 7 2551.0 8 1975.0 9 1884.0 10 1721.0 11 1596.0 12 1505.0 13 1533.0 14 1432.0 15 1487.0 16 1514.0 17 1561.0 18 1789.0 19 1933.0 20 2450.0 21 3133.0 22 4035.0 23 5000.0 24 5959.0 25 7120.0 26 8238.0 27 9236.0 28 10869.0 29 13031.0 30 16364.0 31 21669.0 32 29083.0 33 38819.0 34 52755.0 35 74247.0 36 120500.0 37 282530.0 38 965854.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.46362788528795 20.51241548146421 13.819130333411051 24.204826299836792 2 14.617432407410641 21.36329917748812 42.40943693512719 21.609831479974055 3 18.918940950619735 26.302777352399843 30.691202595510607 24.087079101469815 4 12.601583881674722 18.23286554475048 39.205438770162466 29.96011180341233 5 12.295043775248232 38.94395416994767 36.32635041631915 12.434651638484944 6 30.145833491086144 38.721625609192806 18.53361532739872 12.598925572322333 7 26.698189816977763 32.18332282820563 23.375676182642422 17.74281117217419 8 23.832612054560837 38.99397341872156 20.956177944949765 16.217236581767843 9 26.318125943058217 14.809873637481138 21.123338906722438 37.7486615127382 10 14.996584060931268 29.34801284369793 35.27380804693902 20.381595048431784 11 33.26957708875475 21.945990462190064 26.724039521105958 18.06039292794923 12 23.78924150852381 24.749517327843044 33.00760973290781 18.45363143072533 13 29.381903637296787 22.74666385486931 26.156706049306266 21.71472645852764 14 19.842307006908023 23.731694789107895 28.49905714987881 27.92694105410527 15 21.81661800347766 33.8261378975406 23.500069297002295 20.857174801979447 16 20.457336441169947 28.474960972505198 30.241955910722098 20.825746675602762 17 20.450178184613236 29.894020916311465 29.055155707730652 20.60064519134465 18 20.198235884631423 27.123681397130927 32.98096833023941 19.697114387998237 19 23.25600489125688 26.503799458467984 28.797158412670687 21.443037237604447 20 23.40409550272622 27.553749979620306 29.583741897617088 19.45841262003638 21 21.913310313906567 26.762878554084978 28.294854401155238 23.028956730853213 22 22.76530180872113 29.340563870064624 28.374363698404398 19.519770622809848 23 20.823873512170778 28.97503189505232 29.619279925885856 20.581814666891045 24 22.778250737487173 27.000532225248907 30.258344697953437 19.962872339310483 25 21.656898035521095 28.212497714571192 29.85899632118747 20.271607928720243 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 304.0 1 304.0 2 525.5 3 747.0 4 747.0 5 747.0 6 1891.0 7 3035.0 8 3035.0 9 3035.0 10 3941.5 11 4848.0 12 4848.0 13 4848.0 14 7047.0 15 9246.0 16 9246.0 17 9246.0 18 16621.5 19 23997.0 20 23997.0 21 23997.0 22 38464.0 23 52931.0 24 52931.0 25 52931.0 26 78515.5 27 104100.0 28 104100.0 29 104100.0 30 134415.5 31 164731.0 32 164731.0 33 164731.0 34 178853.0 35 192975.0 36 192975.0 37 192975.0 38 198488.0 39 204001.0 40 204001.0 41 204001.0 42 209462.0 43 214923.0 44 214923.0 45 214923.0 46 215935.0 47 216947.0 48 216947.0 49 216947.0 50 203171.5 51 189396.0 52 189396.0 53 189396.0 54 162063.5 55 134731.0 56 134731.0 57 134731.0 58 113056.5 59 91382.0 60 91382.0 61 91382.0 62 74430.5 63 57479.0 64 57479.0 65 57479.0 66 43788.0 67 30097.0 68 30097.0 69 30097.0 70 21859.0 71 13621.0 72 13621.0 73 13621.0 74 9484.0 75 5347.0 76 5347.0 77 5347.0 78 3582.5 79 1818.0 80 1818.0 81 1818.0 82 1184.5 83 551.0 84 551.0 85 551.0 86 361.0 87 171.0 88 171.0 89 171.0 90 103.0 91 35.0 92 35.0 93 35.0 94 23.5 95 12.0 96 12.0 97 12.0 98 7.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10638006739151068 2 2.9113318935826676E-4 3 0.0 4 0.0073947830096999755 5 0.027657652989035345 6 0.035285342550221935 7 0.046872443486680945 8 0.06003166364567461 9 0.054558359685739195 10 0.027715879626906994 11 0.027424746437548726 12 0.05479126623722581 13 0.04949264219090535 14 0.017060404896394432 15 0.010539021454769256 16 0.002794878617839361 17 0.006230250252266908 18 0.004599904391860615 19 0.004483451116117308 20 0.001805025774021254 21 0.002154385601251174 22 0.004716357667603921 23 0.004425224478245655 24 0.006346703528010216 25 0.0014556659467913338 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1717427.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.46786582370503 #Duplication Level Percentage of deduplicated Percentage of total 1 83.84852624138503 55.73232591727789 2 11.059900413683815 14.702559534405509 3 2.569831709151359 5.1243368770012525 4 0.9790800316624733 2.6030944070084057 5 0.44304674869034744 1.4724185922789388 6 0.2535966202163128 1.0113615675529775 7 0.15325577552436057 0.7130609026982738 8 0.10947317349657479 0.5821158565812412 9 0.08300889354854742 0.4965681598703158 >10 0.4310968789905388 5.255286499777972 >50 0.03349825771037921 1.5249320791815084 >100 0.02912599824005488 4.026635121785546 >500 0.003410814004100046 1.5899313560718442 >1k 0.00253624631074106 2.780853943613209 >5k 6.121973853512902E-4 2.3845191848951424 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8127 0.47320788598292673 No Hit GTCCTACAGTGGACATTTCTAAATT 5809 0.33823853939643433 No Hit CTGTAGGACGTGGAATATGGCAAGA 5679 0.3306690764731194 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5564 0.32397301311787924 No Hit GGTATCAACGCAGAGTACTTTTTTT 5409 0.314947884247773 No Hit TATCAACGCAGAGTACTTTTTTTTT 5290 0.3080189143410462 No Hit CTTTAGGACGTGAAATATGGCGAGG 5142 0.29940137193604155 No Hit GTCCTACAGTGTGCATTTCTCATTT 3363 0.1958161831623702 No Hit CTGAAGGACCTGGAATATGGCGAGA 2878 0.16757626379461835 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2569 0.14958423269227744 No Hit CTGTAGGACCTGGAATATGGCGAGA 2562 0.14917664622717589 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2448 0.1425388095098074 No Hit ATTTAGAAATGTCCACTGTAGGACG 2346 0.13659969244689876 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2115 0.12314933909854683 No Hit GAATATGGCAAGAAAACTGAAAATC 2089 0.12163544651388385 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2005 0.11674440893266497 No Hit GGAATATGGCGAGAAAACTGAAAAT 1799 0.10474972153110439 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1753 0.10207129618900831 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.822663787165335E-5 2 0.0 0.0 0.0 0.0 5.822663787165335E-5 3 0.0 0.0 0.0 0.0 5.822663787165335E-5 4 0.0 0.0 0.0 0.0 5.822663787165335E-5 5 0.0 0.0 0.0 0.0 5.822663787165335E-5 6 0.0 0.0 0.0 0.0 5.822663787165335E-5 7 0.0 0.0 0.0 0.0 5.822663787165335E-5 8 0.0 0.0 0.0 0.0 5.822663787165335E-5 9 0.0 0.0 0.0 5.822663787165335E-5 5.822663787165335E-5 10 0.0 0.0 0.0 5.822663787165335E-5 5.822663787165335E-5 11 0.0 0.0 0.0 5.822663787165335E-5 5.822663787165335E-5 12 0.0 0.0 0.0 5.822663787165335E-5 5.822663787165335E-5 13 0.0 0.0 0.0 5.822663787165335E-5 5.822663787165335E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1435 0.0 14.846525 1 CACTGCG 40 0.0052631996 14.256602 7 AGACGTG 40 0.0052631996 14.256602 6 GCGCCAC 65 5.4577664E-5 13.152656 13 TAGGACC 1030 0.0 12.639196 4 CTGCGTC 55 0.003062718 12.094043 9 TTCGATT 55 0.0030700793 12.090169 12 GGCGAGG 1475 0.0 11.908279 19 CACCTTT 790 0.0 11.537545 14 GTCTAGG 100 1.9029612E-6 11.413266 1 CCACCTT 820 0.0 11.236789 13 CGGGGGA 85 5.356816E-5 11.169602 17 TAACGTG 60 0.005860943 11.087175 5 TAGAAAT 795 0.0 10.877033 4 CTGCGGC 70 0.001488318 10.859957 9 TTCCACC 810 0.0 10.672186 11 AGGACCT 1980 0.0 10.655208 5 GGACCTG 1870 0.0 10.571741 6 AAATGTC 785 0.0 10.53354 7 ACCTTTT 885 0.0 10.51331 15 >>END_MODULE