FastQCFastQC Report
Thu 26 May 2016
SRR1512254_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512254_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2245604
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63570.28308642129244516No Hit
GTCCTACAGTGGACATTTCTAAATT56810.2529831617685041No Hit
CTGTAGGACGTGGAATATGGCAAGA51970.2314299404525464No Hit
GTCCTAAAGTGTGTATTTCTCATTT50760.22604163512355696No Hit
CTTTAGGACGTGAAATATGGCGAGG43530.19384539749662005No Hit
TATCAACGCAGAGTACTTTTTTTTT37390.16650308781067366No Hit
GGTATCAACGCAGAGTACTTTTTTT37050.16498901854467662No Hit
GTCCTACAGTGTGCATTTCTCATTT32890.14646393576071293No Hit
CTGAAGGACCTGGAATATGGCGAGA24150.10754344933478921No Hit
CTGTAGGACCTGGAATATGGCGAGA23030.10255592704679899No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12500.013.2271311
TATTGCG1207.3123374E-1012.67760916
TCCAACG1950.012.66909818
TAGGACC11700.012.5890144
ATTGCGA1155.2586984E-912.402287517
TTGCGAG1052.7149508E-711.76416218
GATATAC4550.011.4862351
TATGTCG1104.916128E-711.23697216
AGGACCT21400.011.145665
TCGTATG600.00586374611.0867012
GGACGTA951.3565839E-511.0030976
TGTAGGA35350.010.9948552
GGCGAGG12550.010.90615219
TAGTACC1502.1245796E-910.7696984
AGGGACG803.762269E-410.6905095
GTCTTAG2500.010.642521
ATAGGAC2800.010.5210533
GTAGGAC34350.010.4573253
CCAACGA1553.6325218E-910.42485519
GTGTAGG5200.010.4159271